Gelatin functionalized graphene oxide for mineralization of hydroxyapatite: biomimetic and in vitro evaluation

Nanoscale ◽  
2014 ◽  
Vol 6 (10) ◽  
pp. 5315 ◽  
Author(s):  
Hongyan Liu ◽  
Ju Cheng ◽  
Fengjuan Chen ◽  
Decheng Bai ◽  
Changwei Shao ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
In Vitro Evaluation

scholarly journals Functionalized Graphene Oxide Mediated Adriamycin Delivery and miR-21 Gene Silencing to Overcome Tumor Multidrug Resistance In Vitro

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e60034 ◽  
Author(s):  
Feng Zhi ◽  
Haifeng Dong ◽  
Xuefeng Jia ◽  
Wenjie Guo ◽  
Huiting Lu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Multidrug Resistance ◽  
Gene Silencing ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide

scholarly journals Fabrication and Characterization of a Biomaterial Based on Extracellular-Vesicle Functionalized Graphene Oxide

2021 ◽  
Vol 9 ◽  
Author(s):  
Julia Driscoll ◽  
Anuradha Moirangthem ◽  
Irene K. Yan ◽  
Tushar Patel
Keyword(s):  
Graphene Oxide ◽  
Stem Cell ◽  
Biological Effects ◽  
Organ Injury ◽  
Hepatocyte Proliferation ◽  
Therapeutic Effects ◽  
Solid Organ ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide

Mesenchymal stem cell (MSC) derived extracellular vesicles (EV) are emerging as acellular therapeutics for solid organ injury and as carriers for drug delivery. Graphene-based materials are novel two-dimensional crystal structure-based materials with unique characteristics of stiffness, strength and elasticity that are being explored for various structural and biological applications. We fabricated a biomaterial that would capture desirable properties of both graphene and stem cell derived EV. Metabolically engineered EV that express azide groups were cross-linked with alkyne-functionalized graphene oxide (GO) via a copper catalyzed alkyne-azide cycloaddition (CuAAC) reaction. The crosslinking between EV and GO was accomplished without the need for ligand expression on the metal. Scanning electron and fluorescence microscopy demonstrated excellent cross-linking between EV and GO. Biological effects were assessed by phagocytosis studies and cell viability studies. The uptake of GO or sonicated GO (sGO) resulted in a durable pro-inflammatory immune response. Cell studies further showed that crosslinked GO-EV scaffolds exhibited cell-type dependent cytotoxicity on liver cancer cells whereas there was minimal impact on healthy hepatocyte proliferation. In vitro, neither GO-EV nor sGO-EV induced DNA strand breaks. In vivo studies in zebrafish revealed gross developmental malformations but treatment-induced mortality was only seen with the highest doses of GO-EV and sGO-EV. With these advantages, this engineered biomaterial combining the versatility of graphene with the therapeutic effects of MSC-EV has potential for applications in tissue engineering and regenerative medicine.

scholarly journals XYLOGLUCAN CONJUGATED FUNCTIONALIZED GRAPHENE OXIDE AS A NANO CARRIER SYSTEM FOR pH RESPONSIVE TARGETED DRUG DELIVERY OF FUCOIDAN

2021 ◽  
pp. 144-153
Author(s):  
K. SONIA ◽  
D. RAJESH ◽  
S. ARUNA SHARMILI ◽  
K. S. MEENA
Keyword(s):  
Drug Delivery ◽  
Graphene Oxide ◽  
Hemolytic Activity ◽  
Targeted Delivery ◽  
Drug Loading ◽  
Potential Candidate ◽  
Functionalized Graphene ◽  
Ph Responsive ◽  
Functionalized Graphene Oxide

Objective: Marine polysaccharides are materializing in the field of biomedicine owing to its promising properties, including high biocompatibility, excellent biodegradability, nontoxic nature, abundance and low cost. Fucoidan (FU), a sulphated marine polysaccharide extracted from brown seaweed, shows a promising application prospect as an anticancer model drug. In order to enhance the stability, biocompatibility and drug loading capacity, xyloglucan was chosen as a targeting ligand, conjugated onto the surface of chitosan functionalized graphene oxide for targeted delivery of fucoidan. Methods: Firstly, Graphene oxide (GO) was prepared by modified Hummer’s method and functionalized with chitosan (CS) via amidation process, further conjugated with xyloglucan (XG). The resulting conjugate, GO-CS-XG, was used to deliver fucoidan through a nanocarrier drug delivery method. The developed GO-CS-XG-FU nanosystem was analyzed for its physiochemical characterization, morphology, hemolytic activity, anti-inflammatory and anticancer activity. Results: The FU loading efficiency and capacity were 75.7% and 83.4%, respectively. XG ligands on the nanoparticle may lead the nanoparticles to actively target cancer cells. Hemolytic activity of the FU-loaded GO-CS-XG nanosystem shows negligible activity, thus making it a potential candidate for biomedical applications. In vitro drug release analysis of FU from GO-CS-XG was lesser at physiological pH but under acidic conditions, it was significantly increased. Results of in vitro cell viability studies indicate that the efficiency of fucoidan was improved upon conjugation with the nanosystem (GO-CS-XG) against human histiocytic lymphoma (U 937) cell line. Conclusion: As a result, we propose a new multifunctional graphene-based targeted platform by using xyloglucan polysaccharide as targeting nanomaterial for pH-responsive anticancer drug delivery with high efficacy.

scholarly journals Functionalized graphene oxide for anti-VEGF siRNA delivery: preparation, characterization and evaluation in vitro and in vivo

RSC Advances ◽  
2017 ◽  
Vol 7 (33) ◽  
pp. 20553-20566 ◽  
Author(s):  
Lulu Ren ◽  
Yifan Zhang ◽  
Chunying Cui ◽  
Yanzhao Bi ◽  
Xu Ge
Keyword(s):  
Graphene Oxide ◽  
Tumor Growth ◽  
Tumor Targeting ◽  
Sirna Delivery ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
System P ◽  
Targeting Delivery

GO–PLL–SDGR/VEGF-siRNA inhibits tumor growth as a tumor targeting delivery system.

Water-Soluble Pillararene-Functionalized Graphene Oxide for In Vitro Raman and Fluorescence Dual-Mode Imaging

ChemPlusChem ◽  
2014 ◽  
Vol 79 (3) ◽  
pp. 462-469 ◽  
Author(s):  
Huacheng Zhang ◽  
Xing Ma ◽  
Kim Truc Nguyen ◽  
Yongfei Zeng ◽  
Shuhui Tai ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Water Soluble ◽  
Functionalized Graphene ◽  
Dual Mode ◽  
Functionalized Graphene Oxide
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