scholarly journals Functionalized graphene oxide for anti-VEGF siRNA delivery: preparation, characterization and evaluation in vitro and in vivo

RSC Advances ◽  
2017 ◽  
Vol 7 (33) ◽  
pp. 20553-20566 ◽  
Author(s):  
Lulu Ren ◽  
Yifan Zhang ◽  
Chunying Cui ◽  
Yanzhao Bi ◽  
Xu Ge
Keyword(s):  
Graphene Oxide ◽  
Tumor Growth ◽  
Tumor Targeting ◽  
Sirna Delivery ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
System P ◽  
Targeting Delivery

GO–PLL–SDGR/VEGF-siRNA inhibits tumor growth as a tumor targeting delivery system.

scholarly journals Preparation and Characterization of Functionalized Graphene Oxide Carrier for siRNA Delivery

2018 ◽  
Vol 19 (10) ◽  
pp. 3202 ◽  
Author(s):  
Jing Li ◽  
Xu Ge ◽  
Chunying Cui ◽  
Yifan Zhang ◽  
Yifan Wang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Gene Silencing ◽  
Targeted Delivery ◽  
Mrna Level ◽  
Sirna Delivery ◽  
Tissue Growth ◽  
Vegf Mrna ◽  
Functionalized Graphene Oxide

A successful siRNA delivery system is dependent on the development of a good siRNA carrier. Graphene oxide (GO) has gained great attention as a promising nanocarrier in recent years. It has been reported that GO could be used to deliver a series of drugs including synthetic compounds, proteins, antibodies, and genes. Our previous research indicated that functionalized GO could deliver siRNA into tumor cells and induce a gene silencing effect, to follow up the research, in this research, GO-R8/cRGDfV(GRcR) was designed and prepared for VEGF-siRNA delivery as a novel carrier. The Zeta potential and particle size of the new designed GRcR carrier was measured at (29.46 ± 5.32) mV and (135.7 ± 3.3) nm respectively, and after transfection, the VEGF mRNA level and protein expression level were down-regulated by 48.22% (p < 0.01) and 38.3% (p < 0.01) in HeLa cells, respectively. The fluorescent images of the treated BALB/c nude mice revealed that GRcR/VEGF-siRNA could conduct targeted delivery of VEGF-siRNA into tumor tissues and showed a gene silencing effect as well as a tumor growth inhibitory effect (p < 0.01) in vivo. Further studies showed that GRcR/VEGF-siRNA could effectively inhibit angiogenesis by suppressing VEGF expression. Histology and immunohistochemistry studies demonstrated that GRcR/VEGF-siRNA could inhibit tumor tissue growth effectively and have anti-angiogenesis activity, which was the result of VEGF protein downregulation. Both in vitro and in vivo results demonstrated that GRcR/VEGF-siRNA could be used as an ideal nonviral tumor-targeting vector for VEGF-siRNA delivery in gene therapy.

scholarly journals Functionalized Graphene Oxide Mediated Adriamycin Delivery and miR-21 Gene Silencing to Overcome Tumor Multidrug Resistance In Vitro

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e60034 ◽  
Author(s):  
Feng Zhi ◽  
Haifeng Dong ◽  
Xuefeng Jia ◽  
Wenjie Guo ◽  
Huiting Lu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Multidrug Resistance ◽  
Gene Silencing ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide

A tumor-targeting drug delivery system based on cyclic NGR-modified, combretastatin A4-loaded, functionalized graphene oxide nanosheets

RSC Advances ◽  
2016 ◽  
Vol 6 (72) ◽  
pp. 68134-68140 ◽  
Author(s):  
Fang Ding ◽  
Fanhong Wu ◽  
Qingqing Tian ◽  
Lingling Guo ◽  
Jing Wang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Graphene Oxide ◽  
Drug Delivery System ◽  
Delivery System ◽  
Tumor Targeting ◽  
Functionalized Graphene ◽  
Graphene Oxide Nanosheets ◽  
Functionalized Graphene Oxide ◽  
Combretastatin A4

Graphene oxide has shown great potential in drug delivery.

Dual-functionalized graphene oxide for enhanced siRNA delivery to breast cancer cells

2016 ◽  
Vol 147 ◽  
pp. 315-325 ◽  
Author(s):  
Rana Imani ◽  
Wei Shao ◽  
Samira Taherkhani ◽  
Shahriar Hojjati Emami ◽  
Satya Prakash ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Graphene Oxide ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Sirna Delivery ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide

scholarly journals Microencapsulated Multi-functionalized Graphene Oxide Equipped With Chloroquine for Efficient and Sustained Sirna Delivery

2021 ◽  
Author(s):  
Rana Imani ◽  
Satya Prakash ◽  
Hojatollah Vali ◽  
John F. Presley ◽  
Shahab Faghihi
Keyword(s):  
Graphene Oxide ◽  
Cellular Uptake ◽  
Sirna Delivery ◽  
Layer By Layer ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
Lysosomal Ph ◽  
Intracellular Targeting ◽  
Lysosomotropic Agent ◽  
Layer Coating

Abstract A multi-functionalized graphene oxide (GO)-based carrier with conjugation of aminated-polyethylenglycole (PEG-diamine), octaarginine (R8) and folic acid (FA), which also contains chloroquine (CQ), a lysosomotropic agent, is introduced. The cellular uptake mechanisms and intracellular targeting of FA functionalized nanocarriers are examined. The localized releases of CQ and siRNA intracellular delivery are evaluated. Microencapsulation of the nanocarrier complexed with genes in layer-by-layer coating of alginate micro-beads is also investigated. The covalently co-conjugated FA with PEG and R8 provides a stable formulation with increased cellular uptake compared to FA-free carrier. The CQ-equipped nanocarrier shows a 95% release of CQ at lysosomal pH. The localized release of the drug inside the lysosomes is verified which accelerates the cargo discharge into cytoplasm.

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