Modulating glyoxalase I metal selectivity by deletional mutagenesis: underlying structural factors contributing to nickel activation profiles

Metallomics ◽  
2015 ◽  
Vol 7 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Uthaiwan Suttisansanee ◽  
Yanhong Ran ◽  
Kadia Y. Mullings ◽  
Nicole Sukdeo ◽  
John F. Honek
Keyword(s):  
Protein Engineering ◽  
Glyoxalase I ◽  
Structural Factors ◽  
Metal Selectivity

Switching between the two metal activation classes of glyoxalase I by protein engineering using deletional mutagenesis.

Insights into the biochemical and functional characterization of sortase E transpeptidase of Corynebacterium glutamicum

2019 ◽  
Vol 476 (24) ◽  
pp. 3835-3847 ◽  
Author(s):  
Aliyath Susmitha ◽  
Kesavan Madhavan Nampoothiri ◽  
Harsha Bajaj
Keyword(s):  
Protein Engineering ◽  
Cell Attachment ◽  
Functional Characterization ◽  
Protein Structures ◽  
Structural Similarity ◽  
Surface Proteins ◽  
Sortase A ◽  
Peptide Cleavage ◽  
New Findings

Most Gram-positive bacteria contain a membrane-bound transpeptidase known as sortase which covalently incorporates the surface proteins on to the cell wall. The sortase-displayed protein structures are involved in cell attachment, nutrient uptake and aerial hyphae formation. Among the six classes of sortase (A–F), sortase A of S. aureus is the well-characterized housekeeping enzyme considered as an ideal drug target and a valuable biochemical reagent for protein engineering. Similar to SrtA, class E sortase in GC rich bacteria plays a housekeeping role which is not studied extensively. However, C. glutamicum ATCC 13032, an industrially important organism known for amino acid production, carries a single putative sortase (NCgl2838) gene but neither in vitro peptide cleavage activity nor biochemical characterizations have been investigated. Here, we identified that the gene is having a sortase activity and analyzed its structural similarity with Cd-SrtF. The purified enzyme showed a greater affinity toward LAXTG substrate with a calculated KM of 12 ± 1 µM, one of the highest affinities reported for this class of enzyme. Moreover, site-directed mutation studies were carried to ascertain the structure functional relationship of Cg-SrtE and all these are new findings which will enable us to perceive exciting protein engineering applications with this class of enzyme from a non-pathogenic microbe.

Protektion vor diabetischer Nephropathie durch Glyoxalase I

2013 ◽  
Vol 8 (S 01) ◽  
Author(s):  
S Müller-Krebs ◽  
S Schmidt ◽  
A Erhardt ◽  
PP Nawroth ◽  
V Schwenger
Keyword(s):  
Glyoxalase I

Die Reduktion der Glyoxalase-I ist ein möglicher Mechanismus bei der Entstehung einer CCl-4-induzierten Zirrhose

2014 ◽  
Vol 52 (08) ◽  
Author(s):  
M Hollenbach ◽  
A Thonig ◽  
S Pohl ◽  
C Ripoll ◽  
A Zipprich
Keyword(s):  
Glyoxalase I

Die Hemmung der Glyoxalase-I durch Ethylpyruvat vermindert die LPS-induzierte Aktivierung von hepatischen Sternzellen

2015 ◽  
Vol 53 (01) ◽  
Author(s):  
M Hollenbach ◽  
A Thonig ◽  
S Pohl ◽  
C Ripoll ◽  
A Zipprich
Keyword(s):  
Glyoxalase I

Glyoxalase I: Implications for an enzyme involved in trait anxiety

2005 ◽  
Vol 38 (05) ◽  
Author(s):  
L Czibere ◽  
MS Keßler ◽  
I Birg ◽  
M Panhuysen ◽  
B Pütz ◽  
...  
Keyword(s):  
Trait Anxiety ◽  
Glyoxalase I

Protein Engineering of Penicillin Acylase

Acta Naturae ◽  
2010 ◽  
Vol 2 (3) ◽  
pp. 47-61 ◽  
Author(s):  
V I Tishkov ◽  
S S Savin ◽  
A S Yasnaya
Keyword(s):  
Protein Engineering ◽  
Penicillin Acylase

Sociostructural Aspects of Socio-Economic Development:The role of Economic approach

2014 ◽  
pp. 86-105
Author(s):  
M. Shabanova
Keyword(s):  
Economic Development ◽  
Structural Factors ◽  
Economic Approach ◽  
Economic Agents ◽  
Socio Economic Development

The author discusses the importance of studying socio-structural factors of socio-economic development through a broader application of the economic approach. The resources of status positions of economic agents are in the spotlight. A possible platform for interdisciplinary interactions is proposed which allows to increase the contribution of both economics and sociology in improving governance at all levels.

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