scholarly journals Inhibition of clot formation in deterministic lateral displacement arrays for processing large volumes of blood for rare cell capture

Lab on a Chip ◽  
2015 ◽  
Vol 15 (10) ◽  
pp. 2240-2247 ◽  
Author(s):  
Joseph D'Silva ◽  
Robert H. Austin ◽  
James C. Sturm
Keyword(s):  
Lateral Displacement ◽  
Clot Formation ◽  
Cell Capture ◽  
Deterministic Lateral Displacement ◽  
Rare Cell Capture

Disabling of mechanisms driving clot formation in deterministic lateral displacement arrays allows rare cell capture from large volumes of blood.

scholarly journals Multidirectional sorting modes in deterministic lateral displacement devices

2008 ◽  
Vol 78 (4) ◽  
Author(s):  
Brian R. Long ◽  
Martin Heller ◽  
Jason P. Beech ◽  
Heiner Linke ◽  
Henrik Bruus ◽  
...  
Keyword(s):  
Lateral Displacement ◽  
Deterministic Lateral Displacement

Separation of viable and nonviable mammalian cells using a deterministic lateral displacement microfluidic device

2016 ◽  
Vol 10 (1) ◽  
pp. 014125 ◽  
Author(s):  
Naotomo Tottori ◽  
Takasi Nisisako ◽  
Jongho Park ◽  
Yasuko Yanagida ◽  
Takeshi Hatsuzawa
Keyword(s):  
Microfluidic Device ◽  
Mammalian Cells ◽  
Lateral Displacement ◽  
Deterministic Lateral Displacement

Characterizing the High Reynolds Number Regime for Deterministic Lateral Displacement (DLD) Devices

2017 ◽  
Author(s):  
Brian Dincau ◽  
Arian Aghilinejad ◽  
Jong-Hoon Kim ◽  
Xiaolin Chen
Keyword(s):  
Reynolds Number ◽  
Laminar Flow ◽  
Soft Lithography ◽  
Lateral Displacement ◽  
Flow Characteristics ◽  
Critical Diameter ◽  
Flow Rates ◽  
Deterministic Lateral Displacement ◽  
Array Configuration ◽  
High Reynolds

Deterministic lateral displacement (DLD) is a common name given to a class of continuous microfluidic separation devices that use a repeating array of pillars to selectively displace particles having a mean diameter greater than the critical diameter (Dc). This Dc is an emergent property influenced by pillar shape, size, and spacing, in addition to the suspending fluid and target particle properties. The majority of previous research in DLD applications has focused on the utilization of laminar flow in low Reynolds number (Re) regimes. While laminar flow exhibits uniform streamlines and predictable separation characteristics, this low-Re regime is dependent on relatively low fluid velocities, and may not hold true at higher processing speeds. Through numerical modeling and experimentation, we investigated high-Re flow characteristics and potential separation enhancements resulting from vortex generation within a DLD array. We used an analytical model and computational software to simulate DLD performance spanning a Re range of 1–100 at flow rates of 2–170 μL/s (0.15–10 mL/min). Each simulated DLD array configuration was composed of 60 μm cylindrical pillars with a 45 μm gap size. The experimental DLD device was fabricated using conventional soft lithography, and injected with 20 μm particles at varying flow rates to observe particle trajectories. The simulated results predict a shift in Dc at Re > 50, while the experimental results indicate a breakdown of typical DLD operation at Re > 70.

Scaling of deterministic lateral displacement devices to a single column of bumping obstacles

Lab on a Chip ◽  
2020 ◽  
Vol 20 (18) ◽  
pp. 3461-3467
Author(s):  
Weibin Liang ◽  
Robert H. Austin ◽  
James C. Sturm
Keyword(s):  
Lateral Displacement ◽  
Deterministic Lateral Displacement ◽  
Single Column

Scaling DLD array devices to a single column of bumping obstacles to increase throughput per area and minimize device area.

scholarly journals Deterministic Lateral Displacement: Challenges and Perspectives

ACS Nano ◽  
2020 ◽  
Vol 14 (9) ◽  
pp. 10784-10795 ◽  
Author(s):  
Axel Hochstetter ◽  
Rohan Vernekar ◽  
Robert H. Austin ◽  
Holger Becker ◽  
Jason P. Beech ◽  
...  
Keyword(s):  
Lateral Displacement ◽  
Deterministic Lateral Displacement

scholarly journals Accelerated Particle Separation in a DLD Device at Re > 1 Investigated by Means of µPIV

Micromachines ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 768 ◽  
Author(s):  
Jonathan Kottmeier ◽  
Maike Wullenweber ◽  
Sebastian Blahout ◽  
Jeanette Hussong ◽  
Ingo Kampen ◽  
...  
Keyword(s):  
Lateral Displacement ◽  
Fluid Dynamic ◽  
Particle Diameter ◽  
Reynolds Numbers ◽  
Field Pattern ◽  
Cfd Simulations ◽  
Deterministic Lateral Displacement ◽  
Tracer Particles ◽  
Image Velocimetry ◽  
Microparticle Image Velocimetry

A pressure resistant and optically accessible deterministic lateral displacement (DLD) device was designed and microfabricated from silicon and glass for high-throughput fractionation of particles between 3.0 and 7.0 µm comprising array segments of varying tilt angles with a post size of 5 µm. The design was supported by computational fluid dynamic (CFD) simulations using OpenFOAM software. Simulations indicated a change in the critical particle diameter for fractionation at higher Reynolds numbers. This was experimentally confirmed by microparticle image velocimetry (µPIV) in the DLD device with tracer particles of 0.86 µm. At Reynolds numbers above 8 an asymmetric flow field pattern between posts could be observed. Furthermore, the new DLD device allowed successful fractionation of 2 µm and 5 µm fluorescent polystyrene particles at Re = 0.5–25.

scholarly journals Active Posts in Deterministic Lateral Displacement Devices

2019 ◽  
Vol 4 (9) ◽  
pp. 1900339 ◽  
Author(s):  
Jason P. Beech ◽  
Kevin Keim ◽  
Bao Dang Ho ◽  
Carlotta Guiducci ◽  
Jonas O. Tegenfeldt
Keyword(s):  
Lateral Displacement ◽  
Deterministic Lateral Displacement
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