Rapid determination of cell mass and density using digitally controlled electric field in a microfluidic chip

Lab on a Chip ◽  
2014 ◽  
Vol 14 (22) ◽  
pp. 4426-4434 ◽  
Author(s):  
Yuliang Zhao ◽  
Hok Sum Sam Lai ◽  
Guanglie Zhang ◽  
Gwo-Bin Lee ◽  
Wen Jung Li
Keyword(s):  
Computer Vision ◽  
Electric Field ◽  
Single Cell ◽  
Cell Density ◽  
Microfluidic Chip ◽  
Rapid Determination ◽  
Cell Mass ◽  
Digitally Controlled ◽  
Optically Induced

Single-cell density and mass were measured rapidly using a combination of optically induced electrokinetics, microfluidics, computer vision, and classical sedimentation theory.

Study of Microfluidic Chip with In Situ Prepared Chitosan Membrane Immobilized with Glucose Oxidase

2009 ◽  
Vol 60-61 ◽  
pp. 194-197
Author(s):  
Qiang Cao ◽  
Yi Xu ◽  
De Fu Song ◽  
Wei Wei Han ◽  
Xi Zhe Dong
Keyword(s):  
Glucose Oxidase ◽  
Microfluidic Chip ◽  
Rapid Determination ◽  
Reaction System ◽  
Chitosan Membrane ◽  
Concentration Time ◽  
Orthogonal Experiments ◽  
Optimized Conditions

A microfluidic chip with in-situ prepared chitosan membrane immobilized with Glucose Oxidase was developed as glucose oxidase reactor to detect human serum. The chitosan was prepared in-situ in the micro-channel as the immobilization material.Then,GOD was immobilized in the chitosan membrane, in which factors, such as the glutaraldehyde concentration, time of crosslinking and immobilization, buffer pH, were optimized with orthogonal experiments. The concentration of glutaraldehyde was finally chosen as 0.15%, time of crosslinking as 60 min, immobilization time as 15 h, and buffer PH as 8.0. The immobilization efficiency could reach up to 5U•cm2. The homemade microchip could be applied for rapid determination of glucose in serum, combining with the Luminol-K3Fe(SCN)3 Chemiluminescence reaction system. Under optimized conditions of sample velocity of 500uL•min-1, the concentration of K3Fe(SCN)3 of 1×10-2mol•L-1 and the concentration of luminol of 5×10-4 mol•L-1, the detection limit could achieved 1ug•mL-1.

scholarly journals Rapid determination of single base mismatch mutations in DNA hybrids by direct electric field control

1997 ◽  
Vol 94 (4) ◽  
pp. 1119-1123 ◽  
Author(s):  
R. G. Sosnowski ◽  
E. Tu ◽  
W. F. Butler ◽  
J. P. O'Connell ◽  
M. J. Heller
Keyword(s):  
Electric Field ◽  
Rapid Determination ◽  
Single Base ◽  
Single Base Mismatch ◽  
Base Mismatch ◽  
Field Control
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