Integrated fluorescence detection of labeled biomolecules using a prism-like PDMS microfluidic chip and lateral light excitation

Lab on a Chip ◽  
2014 ◽  
Vol 14 (12) ◽  
pp. 1991 ◽  
Author(s):  
Pedro Novo ◽  
Virginia Chu ◽  
João Pedro Conde
Keyword(s):  
Fluorescence Detection ◽  
Microfluidic Chip ◽  
Light Excitation

Multiplex enumeration of Escherichia coli and Salmonella enteritidis in a passive capillary microfluidic chip

2020 ◽  
Vol 12 (30) ◽  
pp. 3788-3796
Author(s):  
Üzeyir Dogan ◽  
Esin Nagihan Kasap ◽  
Ferah Sucularlı ◽  
Ender Yildirim ◽  
Ugur Tamer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fluorescence Detection ◽  
Microfluidic Chip ◽  
Salmonella Enteritidis ◽  
E Coli

The concept of a capillary driven microfluidic chip to carry out the immunoassay for fluorescence detection of two target bacteria, namely Escherichia coli (E. coli) and Salmonella enteritidis (S. enteritidis) in less than 60 min.

The Detection of a Fluorescent Dye by Surface-Enhanced Fluorescence with the Addition of Silver Nanoparticles and Its Application for the Space Station

2020 ◽  
Vol 20 (5) ◽  
pp. 3195-3200 ◽  
Author(s):  
Jian Wu ◽  
Yongjun Du ◽  
Chunyan Wang ◽  
Tao Chen
Keyword(s):  
Silver Nanoparticles ◽  
Fluorescence Detection ◽  
Microfluidic Chip ◽  
Fluorescent Dye ◽  
Fluorescence Signal ◽  
Fast Method ◽  
Dye Molecules ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Surface Enhanced

Surface-enhanced fluorescence detection has large potential for detecting many chemical and biological trace analytes. This paper presents a novel method for preparing silver nanomaterials in microfluidic chip channels for the surface-enhanced fluorescence detection of fluorescent dye (SYBR Green I) molecules. Microfluidic chip channels were fabricated by a 248-nm excimer laser. Silver nanoparticles (Ag-NPs) were prepared inside the microfluidic chip channels by directly heating the silver precursor solution. The influence of different temperatures on the sizes of the silver nanoparticles was studied. Then, the surface-enhanced fluorescence technology based on the microfluidic system was used to detect the fluorescent dye molecules. As a result, the fluorescence signal of the fluorescent dye molecules was significantly enhanced by the silver nanoparticles. In addition, the effect of particle size on the fluorescence signal was studied. This simple and fast method is suitable for a fluorescent PCR (polymerase chain reaction) system and has good application prospects for detecting harmful microorganisms in a spacecraft.

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