Zinc complexes as fluorescent chemosensors for nucleic acids: new perspectives for a “boring” element

2015 ◽  
Vol 44 (8) ◽  
pp. 3527-3535 ◽  
Author(s):  
Alessio Terenzi ◽  
Antonino Lauria ◽  
Anna Maria Almerico ◽  
Giampaolo Barone
Keyword(s):  
Nucleic Acids ◽  
Fluorescent Probes ◽  
Zinc Complexes ◽  
Diagnostic Tools ◽  
Fluorescent Chemosensors

Recent applications of zinc(ii) complexes as fluorescent probes for nucleic acids are described highlighting their potential as diagnostic tools.

2′-BIS-PYRENE MODIFIED OLIGONUCLEOTIDES: SENSITIVE FLUORESCENT PROBES OF NUCLEIC ACIDS STRUCTURE

2005 ◽  
Vol 24 (5-7) ◽  
pp. 729-734 ◽  
Author(s):  
D. S. Novopashina ◽  
D. A. Stetsenko ◽  
O. S. Totskaya ◽  
M. N. Repkova ◽  
A. G. Venyaminova
Keyword(s):  
Nucleic Acids ◽  
Fluorescent Probes ◽  
Modified Oligonucleotides

Exciton-Controlled Hybridization-Sensitive Fluorescent Probes: Multicolor Detection of Nucleic Acids

2009 ◽  
Vol 48 (35) ◽  
pp. 6480-6484 ◽  
Author(s):  
Shuji Ikeda ◽  
Takeshi Kubota ◽  
Mizue Yuki ◽  
Akimitsu Okamoto
Keyword(s):  
Nucleic Acids ◽  
Fluorescent Probes

Exciton-Controlled Hybridization-Sensitive Fluorescent Probes: Multicolor Detection of Nucleic Acids

2009 ◽  
Vol 121 (35) ◽  
pp. 6602-6606 ◽  
Author(s):  
Shuji Ikeda ◽  
Takeshi Kubota ◽  
Mizue Yuki ◽  
Akimitsu Okamoto
Keyword(s):  
Nucleic Acids ◽  
Fluorescent Probes

scholarly journals Studies on acedan-based mononuclear zinc complexes toward selective fluorescent probes for pyrophosphate

2012 ◽  
Vol 10 (42) ◽  
pp. 8410 ◽  
Author(s):  
Alla Sreenivasa Rao ◽  
Subhankar Singha ◽  
Wonyoung Choi ◽  
Kyo Han Ahn
Keyword(s):  
Fluorescent Probes ◽  
Zinc Complexes

BODIPY-modified uridines as potential fluorescent probes for nucleic acids that are recognized by DNA-polymerases

2008 ◽  
Vol 52 (1) ◽  
pp. 349-350 ◽  
Author(s):  
T. Ehrenschwender ◽  
C. Wanninger-Weiss ◽  
H.-A. Wagenknecht
Keyword(s):  
Nucleic Acids ◽  
Fluorescent Probes ◽  
Dna Polymerases

Studies of monomeric and homodimeric oxazolo[4,5-b]pyridinium cyanine dyes as fluorescent probes for nucleic acids visualization

2006 ◽  
Vol 68 (3) ◽  
pp. 155-165 ◽  
Author(s):  
V.B. Kovalska ◽  
V.P. Tokar ◽  
M.Yu. Losytskyy ◽  
T. Deligeorgiev ◽  
A. Vassilev ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fluorescent Probes ◽  
Cyanine Dyes

scholarly journals A Warm-start Digital CRISPR-based Method for the Quantitative Detection of Nucleic Acids

2021 ◽  
Author(s):  
Xiaolin Wu ◽  
Yie Hou Lee ◽  
Timothy K Lu ◽  
Hanry Yu
Keyword(s):  
Nucleic Acids ◽  
Pathogen Detection ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Great Promise ◽  
Quantitative Detection ◽  
Inhibitor Tolerance ◽  
Digital Devices ◽  
Warm Start ◽  
Dna And Rna

Nucleic acids-based molecular diagnostic tools incorporating the CRISPR/Cas system are being developed as rapid and sensitive methods for pathogen detection. However, most CRISPR/Cas-based diagnostics lack quantitative detection ability. Here, we report Warm-Start RApid DIgital Crispr Approach (WS-RADICA), which uses commercially available digital chips for the rapid, sensitive, and quantitative detection of nucleic acids. WS-RADICA detected as little as 1 copy/μL SARS-CoV-2 RNA in 40 min (qualitative detection) or 60 min (quantitative detection). WS-RADICA can be easily adapted to various digital devices: two digital devices were evaluated for both DNA and RNA quantification, with linear dynamic ranges of 0.8-12777 copies/μL for DNA and 1.2-18391 copies/μL for RNA (both R2 values > 0.99). Moreover, WS-RADICA had greater sensitivity and inhibitor tolerance than a bulk RT-LAMP-Cas12b reaction and similar performance to RT-qPCR and RT-dPCR. Given its speed, sensitivity, quantification capability, and inhibitor tolerance, WS-RADICA shows great promise for a variety of applications requiring nucleic acid quantification.

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