A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction

2015 ◽  
Vol 51 (10) ◽  
pp. 1815-1818 ◽  
Author(s):  
Chunxia Song ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Qing Wang ◽  
Jianbo Liu ◽  
...  
Keyword(s):  
Kinase Activity ◽  
Sensitive Detection ◽  
Activity Assay ◽  
Enhanced Fluorescence ◽  
Cyclodextrin Polymer ◽  
Polynucleotide Kinase ◽  
Kinase Activity Assay ◽  
T4 Polynucleotide Kinase

A strategy for performing a polynucleotide kinase activity assay has been developed based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction.

An amplified fluorescence detection of T4 polynucleotide kinase activity based on coupled exonuclease III reaction and a graphene oxide platform

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1827-1831 ◽  
Author(s):  
Ni-Na Sun ◽  
Rong-Mei Kong ◽  
Fengli Qu ◽  
Xiaobing Zhang ◽  
Shufang Zhang ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Fluorescence Detection ◽  
Kinase Activity ◽  
Sensitive Detection ◽  
Exonuclease Iii ◽  
Sensing System ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A novel amplified fluorescence graphene oxide (GO) sensing system for sensitive detection of T4 polynucleotide kinase (PNK) activity and inhibition was developed based on the exonuclease III (ExoIII) reaction.

Label-free fluorescence assay coupled exonuclease reaction and SYBR Green I for the detection of T4 polynucleotide kinase activity

2020 ◽  
Vol 12 (6) ◽  
pp. 807-812
Author(s):  
Xu Wu ◽  
Shuyi He ◽  
Julia Xiaojun Zhao
Keyword(s):  
Kinase Activity ◽  
Sybr Green ◽  
Fluorescence Assay ◽  
Sybr Green I ◽  
Label Free ◽  
Cleavage Reaction ◽  
Polynucleotide Kinase ◽  
T4 Polynucleotide Kinase

A sensitive label-free fluorescence assay for monitoring T4 polynucleotide kinase (T4 PNK) activity and inhibition was developed based on a coupled λ exonuclease cleavage reaction and SYBR Green I.

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