Protein-based fluorescent metal nanoclusters for small molecular drug screening

2014 ◽  
Vol 50 (89) ◽  
pp. 13805-13808 ◽  
Author(s):  
Yong Yu ◽  
Siu Yee New ◽  
Jianping Xie ◽  
Xiaodi Su ◽  
Yen Nee Tan
Keyword(s):  
Drug Screening ◽  
Screening Method ◽  
Gold Nanoclusters ◽  
Metal Nanoclusters

This communication reports a facile, fast (<10 min), and hitherto unreported drug screening method by comparing the fluorescence intensities of the in situ formed gold nanoclusters templated by drug-loaded proteins.

In situ studies on controlling an atomically-accurate formation process of gold nanoclusters

Nanoscale ◽  
2015 ◽  
Vol 7 (34) ◽  
pp. 14452-14459 ◽  
Author(s):  
Lina Yang ◽  
Hao Cheng ◽  
Yong Jiang ◽  
Ting Huang ◽  
Jie Bao ◽  
...  
Keyword(s):  
Formation Mechanism ◽  
Formation Process ◽  
Gold Nanoclusters ◽  
Metal Nanoclusters ◽  
In Situ Studies ◽  
Molecular Formation

Knowledge of the molecular formation mechanism of metal nanoclusters is essential for developing chemistry for accurate control over their synthesis.

A New Strategy to Construct Gold Nanoclusters-Based Optical Probe Using Luminescence Resonance Energy Transfer

2021 ◽  
Author(s):  
Shuyang Zhai ◽  
Wei Hu ◽  
Chen Fan ◽  
Wenqi Feng ◽  
Zhi-hong Liu
Keyword(s):  
Energy Transfer ◽  
Gold Nanoclusters ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Optical Probe ◽  
Luminescent Materials ◽  
Metal Nanoclusters ◽  
New Strategy ◽  
Photoluminescence Efficiency

Monolayer-protected metal nanoclusters (MPCs) are emerging as intriguing luminescent materials, but the construction of MPCs-based optical probe is still scarce because of both the limited photoluminescence efficiency of MPCs and...

scholarly journals Synthesis of Exosome-Based Fluorescent Gold Nanoclusters for Cellular Imaging Applications

2021 ◽  
Vol 22 (9) ◽  
pp. 4433
Author(s):  
Eun Sung Lee ◽  
Byung Seok Cha ◽  
Seokjoon Kim ◽  
Ki Soo Park
Keyword(s):  
Photoelectron Spectroscopy ◽  
Low Cost ◽  
Stokes Shift ◽  
Gold Nanoclusters ◽  
Human Colon ◽  
Cellular Imaging ◽  
Metal Nanoclusters ◽  
Human Colon Cancer ◽  
X Ray ◽  
High Concentrations

In recent years, fluorescent metal nanoclusters have been used to develop bioimaging and sensing technology. Notably, protein-templated fluorescent gold nanoclusters (AuNCs) are attracting interest due to their excellent fluorescence properties and biocompatibility. Herein, we used an exosome template to synthesize AuNCs in an eco-friendly manner that required neither harsh conditions nor toxic chemicals. Specifically, we used a neutral (pH 7) and alkaline (pH 11.5) pH to synthesize two different exosome-based AuNCs (exo-AuNCs) with independent blue and red emission. Using field-emission scanning electron microscopy, energy dispersive X-ray microanalysis, nanoparticle tracking analysis, and X-ray photoelectron spectroscopy, we demonstrated that AuNCs were successfully formed in the exosomes. Red-emitting exo-AuNCs were found to have a larger Stokes shift and a stronger fluorescence intensity than the blue-emitting exo-AuNCs. Both exo-AuNCs were compatible with MCF-7 (human breast cancer), HeLa (human cervical cancer), and HT29 (human colon cancer) cells, although blue-emitting exo-AuNCs were cytotoxic at high concentrations (≥5 mg/mL). Red-emitting exo-AuNCs successfully stained the nucleus and were compatible with membrane-staining dyes. This is the first study to use exosomes to synthesize fluorescent nanomaterials for cellular imaging applications. As exosomes are naturally produced via secretion from almost all types of cell, the proposed method could serve as a strategy for low-cost production of versatile nanomaterials.

Drug screening method development for paper spray coupled to a triple quadrupole mass spectrometer

2017 ◽  
Vol 9 (34) ◽  
pp. 5037-5043 ◽  
Author(s):  
Rachel Jett ◽  
Christine Skaggs ◽  
Nicholas E. Manicke
Keyword(s):  
Mass Spectrometry ◽  
Drug Screening ◽  
Method Development ◽  
Screening Method ◽  
Direct Analysis ◽  
Triple Quadrupole ◽  
Analysis Method ◽  
Quadrupole Mass ◽  
Triple Quadrupole Mass Spectrometer ◽  
Paper Spray

Paper spray mass spectrometry is a direct analysis method in which compounds are extracted and ionized from biofluids dried on paper.

In-silico drug screening method based on the protein–compound affinity matrix using the factor selection technique

2007 ◽  
Vol 42 (7) ◽  
pp. 966-976
Author(s):  
Sukumaran Murali ◽  
Shinichi Hojo ◽  
Hideki Tsujishita ◽  
Haruki Nakamura ◽  
Yoshifumi Fukunishi
Keyword(s):  
Drug Screening ◽  
In Silico ◽  
Screening Method ◽  
Affinity Matrix ◽  
Selection Technique

scholarly journals Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection

2018 ◽  
Vol 4 (11) ◽  
pp. eaar6280 ◽  
Author(s):  
Aditya Dileep Kurdekar ◽  
L. A. Avinash Chunduri ◽  
C. Sai Manohar ◽  
Mohan Kumar Haleyurgirisetty ◽  
Indira K. Hewlett ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Limit Of Detection ◽  
Gold Nanoclusters ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dependent Manner ◽  
Metal Nanoclusters ◽  
Detection Range ◽  
In Silico Studies ◽  
P24 Antigen ◽  
Hiv 1

We have engineered streptavidin-labeled fluorescent gold nanoclusters to develop a gold nanocluster immunoassay (GNCIA) for the early and sensitive detection of HIV infection. We performed computational simulations on the mechanism of interaction between the nanoclusters and the streptavidin protein via in silico studies and showed that gold nanoclusters enhance the binding to the protein, by enhancing interaction between the Au atoms and the specific active site residues, compared to other metal nanoclusters. We also evaluated the role of glutathione conjugation in binding to gold nanoclusters with streptavidin. As proof of concept, GNCIA achieved a sensitivity limit of detection of HIV-1 p24 antigen in clinical specimens of 5 pg/ml, with a detection range up to1000 pg/ml in a linear dose-dependent manner. GNCIA demonstrated a threefold higher sensitivity and specificity compared to enzyme-linked immunosorbent assay for the detection of HIV p24 antigen. The specificity of the immunoassay was 100% when tested with plasma samples negative for HIV-1 p24 antigen and positive for viruses such as hepatitis B virus, hepatitis C virus, and dengue. GNCIA could be developed into a universal labeling technology using the relevant capture and detector antibodies for the specific detection of antigens of various pathogens in the future.

scholarly journals Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-based Drug Screening

2020 ◽  
Author(s):  
Wei Liao ◽  
Wanren Yang ◽  
Yue Zhang ◽  
Fanhong Zeng ◽  
Jiecheng Xu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Drug Screening ◽  
Screening Method ◽  
Screening Methods ◽  
Spheroid Culture ◽  
3D Print ◽  
Culture Plate ◽  
Hcc Cells

Abstract Background: Cancer is the second leading cause of death globally. However, most of the new anti-cancer agents screened by traditional drug screening methods fail in the clinic because of lack of efficacy. One of the reasons for this dilemma is that the two-dimensional (2D) culture cancer cell lines could not represent the in vivo cancer cells well. Fortunately, the development of a three-dimensional (3D) culture technique helps in this problem. Methods: The high-throughput spheroid culture plate was fabricated by using 3D print technique and agarose. 4 hepatocarcinoma (HCC) cell lines were 3D cultured to screen 19 small molecular agents based on the spheroid culture plate. 3D cultured primary HCC cells and tumor-bearing mice model were established to verify the candidate anti-hepatocarcinoma agent. Cell function experiments and western blotting were conducted to explore the anti-hepatocarcinoma mechanism of the candidate agent. Results: Based on the previous study, we established an in vitro 3D drug screening method by using our invented spheroid culture device and found that CUDC-907 can serve as a potent anti-hepatocarcinoma agent. The study data show that CUDC-907 (fimepinostat), a novel dual acting inhibitor of phosphoinositide 3-kinase (PI3K) and histone deacetylase (HDAC), has potent inhibitory effects on HCC cell lines and primary HCC cells in vitro, Animal studies have shown that CUDC-907 can also suppress HCC cells in vivo. Furthermore, we investigated the antitumor mechanism of CUDC-907 in HCC cells. We found that it inhibits the PI3K/AKT/mTOR pathway and downregulates the expression of c-Myc, leading to the suppression of HCC cells. Conclusion: Our results suggest that CUDC-907 can be a candidate anti-HCC drug, and the 3D in vitro drug screening method based on our novel spheroid culture device is promising for drug screening.

scholarly journals Block Selection Mode of the Fire Flooding Based on Influence Factors Analysis

2017 ◽  
Vol 10 (1) ◽  
pp. 29-36
Author(s):  
Yuan Shi-bao ◽  
Jiang Hai-yan ◽  
Wang Boyi ◽  
Li Qing-qing
Keyword(s):  
Evaluation Model ◽  
Screening Method ◽  
Combustion Process ◽  
Influence Factors ◽  
Grey System ◽  
Factor Screening ◽  
Screening Model ◽  
Complex Process ◽  
Selection Mode

Background: In-situ combustion is a complex process with multi-factors, like geology, development and engineering. All of the factors can affect the ultimate effect of in-situ combustion. Objective: Strengthen the research of candidate reservoir screening method and mode for fire flooding. Method: On the basis of summarizing formers' single factor screening conditions, and according to the strength degree of the influence factors on geology and development. The candidate reservoirs are divided into three types for screening, first, second and not suitable. Then the in-situ combustion process is taken as a grey system on the analysis of the influence factors. The fire flooding evaluation model is established using the method of correlation analysis that selects the main factors. Results: Block selection mode of fire flooding is established and using an example to calculate and evaluate the applicability. Conclusion: This fire flooding reservoir screening model breaks through the traditional single index screening model, and selects out suitable reservoirs for fire flooding, which improved the test success rate. The J function is convenient to calculate the fire flooding effect, and accurately guide the in-situ combustion experimental blocks screening and in-situ combustion project evaluation.

Gold nanoclusters and fluorescence-based sensing

2017 ◽  
Author(s):  
◽  
Xi Chen
Keyword(s):  
Fluorescence Intensity ◽  
Cholesterol Oxidase ◽  
Gold Nanoclusters ◽  
Green Emission ◽  
Mapping Method ◽  
Metal Nanoclusters ◽  
Molecular Rotor ◽  
Great Resistance ◽  
University Of Missouri ◽  
Thermal Reversibility

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] The main topic of this dissertation focuses on development of analytical methods, mainly modification and application of metal nanoclusters in fluorescence-based sensing. Based on bovine serum albumin passivated gold nanoclusters (AuNCs@BSA) we firstly developed a fluorescent biosensor platform, combining cholesterol oxidase with assistance of surfactant, for selective detection of water insoluble analyte, cholesterol in aqueous condition. Moreover, we designed a series of modification strategy to enhence thermal reversibility of AuNCs@BSA, aiming at reducing hysteresis remained during thermal cycles. Taking advantage of optimized thermal reversibility in terms of fully recoverable fluorescence intensity, analytical performance of modified AuNCs@BSA was investigated and an aqueous-phase temperature sensor was achieved. In addition to AuNCs, we developed novel glutathione stabilized copper NCs with green-emission in assistance of ascorbic acid reduction, which showed great resistance to nitro compounds. Further, analytical application of as-prepared Cu NCs to ratiometric sensing on explosive molecules, cooperating with AuNCs@BSA, was investigated. In the last, a novel and facile temperature mapping method using ionic liquid and organic fluorescent molecular rotor were described. Making use of viscosity-sensitive fluorescent dye, temperature was converted to ratio value of fluorescence intensity and recorded, enabling visualized thermal map.

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