Sensitive quantification of messenger RNA with a real-time ligase chain reaction by using a ribonucleotide-modified DNA probe

2014 ◽  
Vol 50 (86) ◽  
pp. 13093-13095 ◽  
Author(s):  
Yanlei Hu ◽  
Hongxia Jia ◽  
Yucong Wang ◽  
Yongqiang Cheng ◽  
Zhengping Li
Keyword(s):  
Real Time ◽  
Messenger Rna ◽  
Dna Probe ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Modified Dna

scholarly journals Expression of DROSHA and DICER genes in peripheral blood leukocytes in lung sarcoidosis

2018 ◽  
Vol 90 (3) ◽  
pp. 21-24
Author(s):  
I E Malysheva ◽  
O V Balan ◽  
E L Tikhonovich ◽  
T O Volkova
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Peripheral Blood ◽  
Messenger Rna ◽  
Peripheral Blood Leukocytes ◽  
Chain Reaction ◽  
Blood Leukocytes ◽  
Healthy Donors ◽  
Polymerase Chain

Aim. To study the expression level of the genes DROSHA and DICER in peripheral blood leukocytes (PBL) of patients with sarcoidosis of the lungs Materials and methods. The study included 32 patients diagnosed with persistent lung sarcoidosis (mean age 41.56±1.27 years) and 36 healthy donors (control; mean age 42.79±1.95 years). The level of expression of messenger RNA (mRNA) of the genes DROSHA and DICER were determined in PBL of healthy donors and patients with sarcoidosis of the lung by polymerase chain reaction in real time. Results. As a result of the conducted researches it is established that the level of drosha gene expression in PBL patients with sarcoidosis of lungs is significantly reduced in comparison with the control (p

Real-time quantification of fusion transcripts with ligase chain reaction by direct ligation of adjacent DNA probes at fusion junction

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3977-3982
Author(s):  
Fengxia Su ◽  
Jianing Ji ◽  
Pengbo Zhang ◽  
Fangfang Wang ◽  
Zhengping Li
Keyword(s):  
Real Time ◽  
Fusion Transcript ◽  
Dna Probes ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Fusion Transcripts ◽  
Fusion Junction

A fusion transcript assay is developed based on direct ligation and ligase chain reaction.

Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe

2011 ◽  
Vol 47 (33) ◽  
pp. 9465 ◽  
Author(s):  
Jiangyan Zhang ◽  
Zhengping Li ◽  
Hui Wang ◽  
Yucong Wang ◽  
Hongxia Jia ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Probe ◽  
Modified Dna ◽  
Mature Micrornas

scholarly journals An Inexpensive Gel Electrophoresis-Based Polymerase Chain Reaction Method for Quantifying mRNA Levels

2005 ◽  
Vol 4 (2) ◽  
pp. 157-168 ◽  
Author(s):  
William D. Bradford ◽  
Laty Cahoon ◽  
Sara R. Freel ◽  
Laura L. Mays Hoopes ◽  
Todd T. Eckdahl
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Gel Electrophoresis ◽  
Messenger Rna ◽  
Polymerase Chain Reaction Method ◽  
Mrna Levels ◽  
Chain Reaction ◽  
Teaching And Research ◽  
Undergraduate Institutions ◽  
Polymerase Chain

In order to engage their students in a core methodology of the new genomics era, an everincreasing number of faculty at primarily undergraduate institutions are gaining access to microarray technology. Their students are conducting successful microarray experiments designed to address a variety of interesting questions. A next step in these teaching and research laboratory projects is often validation of the microarray data for individual selected genes. In the research community, this usually involves the use of real-time polymerase chain reaction (PCR), a technology that requires instrumentation and reagents that are prohibitively expensive for most undergraduate institutions. The results of a survey of faculty teaching undergraduates in classroom and research settings indicate a clear need for an alternative approach. We sought to develop an inexpensive and student-friendly gel electrophoresis-based PCR method for quantifying messenger RNA (mRNA) levels using undergraduate researchers as models for students in teaching and research laboratories. We compared the results for three selected genes measured by microarray analysis, real-time PCR, and the gel electrophoresis-based method. The data support the use of the gel electrophoresis-based method as an inexpensive, convenient, yet reliable alternative for quantifying mRNA levels in undergraduate laboratories.

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