Ultrasensitive quantification of mature microRNAs by real-time PCR based on ligation of a ribonucleotide-modified DNA probe

2011 ◽  
Vol 47 (33) ◽  
pp. 9465 ◽  
Author(s):  
Jiangyan Zhang ◽  
Zhengping Li ◽  
Hui Wang ◽  
Yucong Wang ◽  
Hongxia Jia ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Probe ◽  
Modified Dna ◽  
Mature Micrornas

scholarly journals Evaluation of dual-labeled fluorescent DNA probe purity versus performance in real-time PCR

BioTechniques ◽  
2004 ◽  
Vol 36 (2) ◽  
pp. 266-275 ◽  
Author(s):  
Anthony T. Yeung ◽  
Brian P. Holloway ◽  
Pamela Scott Adams ◽  
Gregory L. Shipley
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Probe

scholarly journals Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR

2007 ◽  
Vol 35 (16) ◽  
pp. e101-e101 ◽  
Author(s):  
S. Huang ◽  
J. Salituro ◽  
N. Tang ◽  
K.-C. Luk ◽  
J. Hackett ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Probe ◽  
Probe Design ◽  
Linear Dna

The importance for standardization and validation of real-time PCR for the detection of mature miR-21 in the blood of patients

2020 ◽  
pp. 66-67
Author(s):  
Л.В. Шуленина ◽  
Д.В. Салеева
Keyword(s):  
Real Time ◽  
Correlation Coefficient ◽  
Real Time Pcr ◽  
Coefficient Of Variation ◽  
Healthy Donors ◽  
Mature Micrornas ◽  
Pcr Method ◽  
Detection And Quantification

Процессы обнаружения и количественного определения методом ПЦР в реальном времени в крови пациентов зрелых микроРНК, в том числе и miR-21, должны быть стандартизированы и валидированы. Проведенные нами исследования, показывают, что специфичность метода ПЦР для miR-21 в крови здоровых доноров может быть демонстрирована образованием продукта-ампликона размером 67 нуклеотидов, прецизионность в условиях сходимости и воспроизводимости, выраженная в виде коэффициента вариации, составляет менее 2% и 3 % соответственно, а линейность метода подтверждается коэффицентом корреляции r≥0,99. The processes of detection and quantification by real-time PCR in blood of mature microRNAs, including miR-21, should be standardized and validated. Our studies show that the specificity of PCR method for miR-21 in the blood of healthy donors can be demonstrated by the formation of a 67 bp amplicon product, precision under conditions of convergence and reproducibility, expressed using the coefficient of variation, is less than 2% and 3%, respectively, and the linearity of PCR method for miR-21 is confirmed by the correlation coefficient r≥0.99.

Sensitive quantification of messenger RNA with a real-time ligase chain reaction by using a ribonucleotide-modified DNA probe

2014 ◽  
Vol 50 (86) ◽  
pp. 13093-13095 ◽  
Author(s):  
Yanlei Hu ◽  
Hongxia Jia ◽  
Yucong Wang ◽  
Yongqiang Cheng ◽  
Zhengping Li
Keyword(s):  
Real Time ◽  
Messenger Rna ◽  
Dna Probe ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Modified Dna

Enzymatic Preparation of Modified DNA: Study of the Kinetics by Real-Time PCR

2019 ◽  
Vol 53 (3) ◽  
pp. 460-469 ◽  
Author(s):  
S. A. Lapa ◽  
A. S. Pavlov ◽  
V. E. Kuznetsova ◽  
V. E. Shershov ◽  
M. A. Spitsyn ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Enzymatic Preparation ◽  
Modified Dna

scholarly journals Quantification of Mature MicroRNAs Using Pincer Probes and Real-Time PCR Amplification

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0120160 ◽  
Author(s):  
Tinghua Huang ◽  
Jun Yang ◽  
Guopin Liu ◽  
Wei Jin ◽  
Zhi Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Amplification ◽  
Mature Micrornas

Feldvalidierung einer real-time PCR zum Nachweis von Mycoplasma hyopneumoniae in Nasentupfermaterial von lebenden Schweinen

2005 ◽  
Vol 147 (9) ◽  
pp. 373-379 ◽  
Author(s):  
F. Zeeh ◽  
P. Kuhnert ◽  
R. Miserez ◽  
M. G. Doherr ◽  
W. Zimmermann
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Mycoplasma Hyopneumoniae
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