scholarly journals Aryloxymaleimides for cysteine modification, disulfide bridging and the dual functionalization of disulfide bonds

2014 ◽  
Vol 50 (54) ◽  
pp. 7139-7142 ◽  
Author(s):  
Cristina Marculescu ◽  
Hanno Kossen ◽  
Rachel E. Morgan ◽  
Patrick Mayer ◽  
Sally A. Fletcher ◽  
...  
Keyword(s):  
Disulfide Bonds ◽  
Cysteine Modification ◽  
Next Generation ◽  
Disulfide Bridging

Aryloxymaleimides represent ‘next generation maleimides’ of attenuated reactivity. We demonstrate their use in establishing novel bioconjugation procedures at disulfide bonds.

scholarly journals Functional native disulfide bridging enables delivery of a potent, stable and targeted antibody–drug conjugate (ADC)

2015 ◽  
Vol 51 (53) ◽  
pp. 10624-10627 ◽  
Author(s):  
João P. M. Nunes ◽  
Maurício Morais ◽  
Vessela Vassileva ◽  
Eifion Robinson ◽  
Vineeth S. Rajkumar ◽  
...  
Keyword(s):  
Blood Serum ◽  
Next Generation ◽  
High Potency ◽  
Antibody Drug Conjugate ◽  
Drug Conjugate ◽  
Disulfide Bridging ◽  
Antibody Drug ◽  
Excellent Stability

A next generation maleimide–ADC is shown to have excellent stability in blood serum, as well as high potency and selectivity in vitro.

scholarly journals Next generation maleimides enable the controlled assembly of antibody–drug conjugates via native disulfide bond bridging

2014 ◽  
Vol 12 (37) ◽  
pp. 7261-7269 ◽  
Author(s):  
Felix F. Schumacher ◽  
João P. M. Nunes ◽  
Antoine Maruani ◽  
Vijay Chudasama ◽  
Mark E. B. Smith ◽  
...  
Keyword(s):  
Disulfide Bond ◽  
Disulfide Bonds ◽  
Next Generation ◽  
Antibody Drug Conjugates ◽  
Controlled Assembly ◽  
Drug Conjugates ◽  
Antibody Drug

Highly homogeneous ADCs are generated by the efficient bridging of interchain disulfide bonds in trastuzumab, using next generation maleimides.

Disulfide bonds and Diel-Alder Reaction Bonds Hybrid Polymers with High Stretchability, Transparency, Recyclability, and Intrinsic Dual-Healability for Skin-like Tactile Sensing

2020 ◽  
Author(s):  
Chun-Ming Yeh ◽  
Chun-Hsiu Lin ◽  
Tzung-You Han ◽  
Yu-Ting Xiao ◽  
Yi-An Chen ◽  
...  
Keyword(s):  
Human Skin ◽  
Disulfide Bonds ◽  
Alder Reaction ◽  
Tactile Sensing ◽  
Next Generation ◽  
Hybrid Polymers ◽  
Electronic Skin

Mimicking the nature of human skin and have additional features by developing the skin-inspired materials and devices for electronic skin (e-skin) applications is crucial to the next generation electronics. However,...

Synthesis of disulfide-bridging trehalose polymers for antibody and Fab conjugation using a bis-sulfone ATRP initiator

2021 ◽  
Vol 12 (9) ◽  
pp. 1217-1223
Author(s):  
Neil L. Forsythe ◽  
Heather D. Maynard
Keyword(s):  
Disulfide Bonds ◽  
Methacrylate Polymers ◽  
Disulfide Bridging

Herein is reported a bis-sulfone ATRP initiator capable of inserting into the disulfide bonds of antibodies. Further, we present trehalose methacrylate polymers made with this initiator that effectively stabilize antibodies to heat as conjugates.

scholarly journals Oxicam-type nonsteroidal anti-inflammatory drugs inhibit NPR1-mediated salicylic acid pathway

2020 ◽  
Author(s):  
Nobuaki Ishihama ◽  
Seung-won Choi ◽  
Yoshiteru Noutoshi ◽  
Ivana Saska ◽  
Shuta Asai ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Disulfide Bonds ◽  
Redox Status ◽  
Inhibitory Effect ◽  
Cysteine Modification ◽  
Pathogenesis Related ◽  
Intermolecular Disulfide Bonds ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Acid Pathway

AbstractSalicylic acid (SA) and its structural analogs are nonsteroidal anti-inflammatory drugs (NSAIDs) that target mammalian cyclooxygenases. In plants, SA acts as a defense hormone that regulates NON-EXPRESSOR OF PATHOGENESIS RELATED GENES 1 (NPR1), the master transcriptional regulator of immunity-related genes. We identified a number of NSAIDs that enhance bacterial effector-induced cell death. Among them, the oxicam-type NSAIDs tenoxicam (TNX), meloxicam, and piroxicam, but not other types of NSAIDs, exhibit an inhibitory effect on immunity to bacteria and SA-dependent immune responses in plants. TNX treatment reduces NPR1 levels, independently from the proposed SA receptors NPR3 and NPR4. Instead, TNX induces oxidation of cytosolic redox status, which is also affected by SA and regulates NPR1 homeostasis. Surprisingly, however, cysteine modification associated with NPR1 oligomerization via intermolecular disulfide bonds is not affected by either SA or TNX. Therefore, oxicam-type NSAIDs highlight importance of SA effects on the cytosolic redox status, but not on cysteine modification or oligomerization of NPR1.

Cryo-electron microscopy of two-dimensional crystals of reduced type B botulinum neurotoxin

1992 ◽  
Vol 50 (1) ◽  
pp. 530-531
Author(s):  
P. F. Flicker ◽  
V.S. Kulkarni ◽  
J. P. Robinson ◽  
G. Stubbs ◽  
B. R. DasGupta
Keyword(s):  
Disulfide Bonds ◽  
Clostridium Botulinum ◽  
Structural Changes ◽  
Two Dimensional ◽  
Type B ◽  
Single Chain ◽  
Muscle Paralysis ◽  
Cryo Electron Microscopy ◽  
Disulfide Linkages ◽  
Intracellular Action

Botulinum toxin is a potent neurotoxin produced by Clostridium botulinum. The toxin inhibits release of neurotransmitter, causing muscle paralysis. There are several serotypes, A to G, all of molecular weight about 150,000. The protein exists as a single chain or or as two chains, with two disulfide linkages. In a recent investigation on intracellular action of neurotoxins it was reported that type B neurotoxin can inhibit the release of Ca++-activated [3H] norepinephrine only if the disulfide bonds are reduced. In order to investigate possible structural changes in the toxin upon reduction of the disulfide bonds, we have prepared two-dimensional crystals of reduced type B neurotoxin. These two-dimensional crystals will be compared with those of the native (unreduced) type B toxin.

1477: Durability of Next Generation Flexible Ureteroscopes: Randomized Prospective Study

2004 ◽  
Vol 171 (4S) ◽  
pp. 389-389
Author(s):  
Manoj Monga ◽  
Ramakrishna Venkatesh ◽  
Sara Best ◽  
Caroline D. Ames ◽  
Courtney Lee ◽  
...  
Keyword(s):  
Prospective Study ◽  
Next Generation ◽  
Flexible Ureteroscopes
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