scholarly journals Site-specific protein labelling and immobilization mediated by microbial transglutaminase

2014 ◽  
Vol 50 (50) ◽  
pp. 6604-6606 ◽  
Author(s):  
Samuel K. Oteng-Pabi ◽  
Christophe Pardin ◽  
Maria Stoica ◽  
Jeffrey W. Keillor
Keyword(s):  
Specific Protein ◽  
Microbial Transglutaminase ◽  
Site Specific ◽  
Protein Labelling ◽  
Target Proteins ◽  
Subsequent Modification

Microbial transglutaminase (mTG) mediates site-specific propargylation of target proteins, allowing their subsequent modification in in vitro bio-conjugation applications.

scholarly journals Peptide-tags for site-specific protein labelling in vitro and in vivo

2016 ◽  
Vol 12 (6) ◽  
pp. 1731-1745 ◽  
Author(s):  
Jonathan Lotze ◽  
Ulrike Reinhardt ◽  
Oliver Seitz ◽  
Annette G. Beck-Sickinger
Keyword(s):  
Metal Ions ◽  
Small Molecules ◽  
Protein Localization ◽  
Specific Protein ◽  
Site Specific ◽  
Protein Labelling ◽  
Peptide Interactions ◽  
Peptide Tags

Peptide-tag based labelling can be achieved by (i) enzymes (ii) recognition of metal ions or small molecules and (iii) peptide–peptide interactions and enables site-specific protein visualization to investigate protein localization and trafficking.

Further Studies on the Site-Specific Protein Modification by Microbial Transglutaminase

2001 ◽  
Vol 12 (5) ◽  
pp. 701-710 ◽  
Author(s):  
Haruya Sato ◽  
Eiko Hayashi ◽  
Naoyuki Yamada ◽  
Masanobu Yatagai ◽  
Yoshiyuki Takahara
Keyword(s):  
Protein Modification ◽  
Specific Protein ◽  
Microbial Transglutaminase ◽  
Site Specific

scholarly journals Site-selective enzymatic chemistry for polymer conjugation to protein lysine residues: PEGylation of G-CSF at lysine-41

2016 ◽  
Vol 7 (42) ◽  
pp. 6545-6553 ◽  
Author(s):  
A. Mero ◽  
A. Grigoletto ◽  
K. Maso ◽  
H. Yoshioka ◽  
A. Rosato ◽  
...  
Keyword(s):  
Specific Protein ◽  
Microbial Transglutaminase ◽  
Site Specific ◽  
Lysine Residues ◽  
Polymer Conjugation ◽  
Site Selective

Microbial transglutaminase (mTGase) is an enzyme that catalyzes site-specific protein derivatization at specific glutamines and lysines.

scholarly journals Strain-promoted sydnone bicyclo-[6.1.0]-nonyne cycloaddition

2014 ◽  
Vol 5 (5) ◽  
pp. 1742-1744 ◽  
Author(s):  
Stephen Wallace ◽  
Jason W. Chin
Keyword(s):  
Specific Protein ◽  
Bioorthogonal Reaction ◽  
Site Specific ◽  
Protein Labelling

We report the strain-promoted sydnone bicyclo-[6.1.0]-nonyne cycloaddition and demonstrate that this bioorthogonal reaction enables site-specific protein labelling.

Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo

2004 ◽  
Vol 14 (24) ◽  
pp. 6067-6070 ◽  
Author(s):  
Lay-Pheng Tan ◽  
Rina Y.P. Lue ◽  
Grace Y.J. Chen ◽  
Shao Q. Yao
Keyword(s):  
Specific Protein ◽  
Site Specific

Site-specific, covalent immobilization of BirA by microbial transglutaminase: A reusable biocatalyst for in vitro biotinylation

2016 ◽  
Vol 511 ◽  
pp. 10-12 ◽  
Author(s):  
Chang-Mei Yu ◽  
Hui Zhou ◽  
Wei-Fen Zhang ◽  
Hong-Ming Yang ◽  
Jin-Bao Tang
Keyword(s):  
Covalent Immobilization ◽  
Microbial Transglutaminase ◽  
Site Specific
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