scholarly journals Genetic Encoding of Bicyclononynes and trans-Cyclooctenes for Site-Specific Protein Labeling in Vitro and in Live Mammalian Cells via Rapid Fluorogenic Diels–Alder Reactions

2012 ◽  
Vol 134 (25) ◽  
pp. 10317-10320 ◽  
Author(s):  
Kathrin Lang ◽  
Lloyd Davis ◽  
Stephen Wallace ◽  
Mohan Mahesh ◽  
Daniel J. Cox ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Specific Protein ◽  
Diels Alder ◽  
Protein Labeling ◽  
Site Specific ◽  
Genetic Encoding

scholarly journals Peptide-tags for site-specific protein labelling in vitro and in vivo

2016 ◽  
Vol 12 (6) ◽  
pp. 1731-1745 ◽  
Author(s):  
Jonathan Lotze ◽  
Ulrike Reinhardt ◽  
Oliver Seitz ◽  
Annette G. Beck-Sickinger
Keyword(s):  
Metal Ions ◽  
Small Molecules ◽  
Protein Localization ◽  
Specific Protein ◽  
Site Specific ◽  
Protein Labelling ◽  
Peptide Interactions ◽  
Peptide Tags

Peptide-tag based labelling can be achieved by (i) enzymes (ii) recognition of metal ions or small molecules and (iii) peptide–peptide interactions and enables site-specific protein visualization to investigate protein localization and trafficking.

scholarly journals Functional genetic encoding of sulfotyrosine in mammalian cells

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Xinyuan He ◽  
Yan Chen ◽  
Daisy Guiza Beltran ◽  
Maia Kelly ◽  
Bin Ma ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Biochemical Characterization ◽  
Trna Synthetase ◽  
Functional Verification ◽  
Cellular Processes ◽  
Genetic Encoding ◽  
Efficient Expression

Abstract Protein tyrosine O-sulfation (PTS) plays a crucial role in extracellular biomolecular interactions that dictate various cellular processes. It also involves in the development of many human diseases. Regardless of recent progress, our current understanding of PTS is still in its infancy. To promote and facilitate relevant studies, a generally applicable method is needed to enable efficient expression of sulfoproteins with defined sulfation sites in live mammalian cells. Here we report the engineering, in vitro biochemical characterization, structural study, and in vivo functional verification of a tyrosyl-tRNA synthetase mutant for the genetic encoding of sulfotyrosine in mammalian cells. We further apply this chemical biology tool to cell-based studies on the role of a sulfation site in the activation of chemokine receptor CXCR4 by its ligand. Our work will not only facilitate cellular studies of PTS, but also paves the way for economical production of sulfated proteins as therapeutic agents in mammalian systems.

Site-Specific Protein Labeling by Intein-Mediated Protein Ligation

2010 ◽  
pp. 87-107 ◽  
Author(s):  
Inca Ghosh ◽  
Nancy Considine ◽  
Elissa Maunus ◽  
Luo Sun ◽  
Aihua Zhang ◽  
...  
Keyword(s):  
Specific Protein ◽  
Protein Labeling ◽  
Site Specific ◽  
Protein Ligation

scholarly journals Site‐Specific Protein Labeling via Sortase‐Mediated Transpeptidation

2009 ◽  
Vol 56 (1) ◽  
Author(s):  
Maximilian Wei‐Lin Popp ◽  
John M. Antos ◽  
Hidde L. Ploegh
Keyword(s):  
Specific Protein ◽  
Protein Labeling ◽  
Site Specific
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