Ratiometric fluorescence assay for γ-glutamyltranspeptidase detection based on a single fluorophore via analyte-induced variation of substitution

2014 ◽  
Vol 50 (26) ◽  
pp. 3417 ◽  
Author(s):  
Xianfeng Hou ◽  
Qingxiang Yu ◽  
Fang Zeng ◽  
Changmin Yu ◽  
Shuizhu Wu
Keyword(s):  
Fluorescence Assay ◽  
Ratiometric Fluorescence ◽  
Induced Variation

Gold nanocluster-europium(III) ratiometric fluorescence assay for dipicolinic acid

2021 ◽  
Vol 188 (1) ◽  
Author(s):  
Xiaoqing Li ◽  
Junjun Luo ◽  
Xingxing Jiang ◽  
Minghui Yang ◽  
Avraham Rasooly
Keyword(s):  
Dipicolinic Acid ◽  
Fluorescence Assay ◽  
Ratiometric Fluorescence ◽  
Gold Nanocluster

scholarly journals A new ratiometric fluorescence assay based on resonance energy transfer between biomass quantum dots and organic dye for the detection of sulfur dioxide derivatives

RSC Advances ◽  
2019 ◽  
Vol 9 (71) ◽  
pp. 41955-41961 ◽  
Author(s):  
Jingjin Zhao ◽  
Yao Peng ◽  
Keqin Yang ◽  
Yunyun Chen ◽  
Shulin Zhao ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Sulfur Dioxide ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Organic Dye ◽  
Fluorescence Assay ◽  
Ratiometric Fluorescence ◽  
Cell Lysates

A new ratiometric fluorescence assay has been developed for the detection of sulfur dioxide derivatives with repeatability and selectivity. The assay was applied to quantitate HSO3− in cell lysates with accurate results.

Ratiometric fluorescence monitoring of cerebral Cu2+ based on coumarin-labeled DNA coupled with the Cu2+-induced oxidation of o-phenylenediamine

The Analyst ◽  
2017 ◽  
Vol 142 (18) ◽  
pp. 3341-3345 ◽  
Author(s):  
Shishi Ma ◽  
Qiao-Yu Zhou ◽  
Fang-Ya Mu ◽  
Zi-Han Chen ◽  
Xu-Yin Ding ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Ratiometric Fluorescence ◽  
Single Stranded Dna

A novel ratiometric fluorescence assay for cerebral Cu2+ has been developed based on coumarin-labeled single-stranded DNA coupled with the Cu2+-induced oxidation of o-phenylenediamine.

scholarly journals A ratiometric fluorescence sensor based on enzymatically activatable micellization of TPE derivatives for quantitative detection of alkaline phosphatase activity in serum

RSC Advances ◽  
2020 ◽  
Vol 10 (45) ◽  
pp. 26888-26894 ◽  
Author(s):  
Jeongmoo Lee ◽  
Seoyun Kim ◽  
Tae Hoon Kim ◽  
Seoung Ho Lee
Keyword(s):  
Aqueous Solution ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Fluorescence Sensor ◽  
Fluorescence Assay ◽  
Quantitative Detection ◽  
Ratiometric Fluorescence ◽  
Alp Activity

A novel ratiometric fluorescence assay via enzymatically activatable micellization in aqueous solution was devised for quantitative detection of alkaline phosphatase (ALP) activity.

A novel dual response ratiometric fluorescent probe for the determination of H2O2 and glucose via etching of silver nanoparticles

The Analyst ◽  
2019 ◽  
Vol 144 (4) ◽  
pp. 1153-1158 ◽  
Author(s):  
Hongzhi Lu ◽  
Chunwei Yu ◽  
Shuai Quan ◽  
Shoufang Xu
Keyword(s):  
Silver Nanoparticles ◽  
Charge Transfer ◽  
Fluorescent Probe ◽  
Inner Filter Effect ◽  
Fluorescence Assay ◽  
Ratiometric Fluorescence ◽  
Ag Nps ◽  
Dual Response ◽  
Ratiometric Fluorescent Probe

Based on the inner filter effect and charge transfer, a dual response ratiometric fluorescence assay for sensing H2O2 and glucose was proposed based on etching of Ag NPs.

scholarly journals Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1313
Author(s):  
Pushap Raj ◽  
Seon-yeong Lee ◽  
Tae Yoon Lee
Keyword(s):  
Bladder Cancer ◽  
Hyaluronic Acid ◽  
Carbon Dots ◽  
Early Recognition ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Assay ◽  
Ratiometric Fluorescence ◽  
Normal Human ◽  
Fluorescence Biosensor

Bladder cancer is the leading cause of death in patients with genitourinary cancer. An elevated level of hyaluronidase (HAase) was found in bladder cancer, which acts as an important biomarker for the early diagnosis of bladder cancer. Hence, there is a need to develop a simple enzymatic assay for the early recognition of HAase. Herein, we report a simple, sensitive, and ratiometric fluorescence assay for HAase detection under physiological conditions. The fluorescence assay was constructed by the adsorption of cationic carbon dots and positively charged naphthalimide on negatively charged hyaluronic acid and the development of a Förster resonance energy transfer (FRET) mechanism from carbon dots to a naphthalimide fluorophores. The hyaluronidase enzyme cleaves the hyaluronic acid in this assay, and breaking down the FRET mechanism induces ratiometric changes. A detection limit of 0.09 U/mL was achieved, which is less than the HAase level found in normal human body fluids. Moreover, this assay may be used for diagnosing HAase-related diseases.

Sign in / Sign up

Export Citation Format

Share Document