scholarly journals Carbon Dot/Naphthalimide Based Ratiometric Fluorescence Biosensor for Hyaluronidase Detection

Materials ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1313
Author(s):  
Pushap Raj ◽  
Seon-yeong Lee ◽  
Tae Yoon Lee
Keyword(s):  
Bladder Cancer ◽  
Hyaluronic Acid ◽  
Carbon Dots ◽  
Early Recognition ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescence Assay ◽  
Ratiometric Fluorescence ◽  
Normal Human ◽  
Fluorescence Biosensor

Bladder cancer is the leading cause of death in patients with genitourinary cancer. An elevated level of hyaluronidase (HAase) was found in bladder cancer, which acts as an important biomarker for the early diagnosis of bladder cancer. Hence, there is a need to develop a simple enzymatic assay for the early recognition of HAase. Herein, we report a simple, sensitive, and ratiometric fluorescence assay for HAase detection under physiological conditions. The fluorescence assay was constructed by the adsorption of cationic carbon dots and positively charged naphthalimide on negatively charged hyaluronic acid and the development of a Förster resonance energy transfer (FRET) mechanism from carbon dots to a naphthalimide fluorophores. The hyaluronidase enzyme cleaves the hyaluronic acid in this assay, and breaking down the FRET mechanism induces ratiometric changes. A detection limit of 0.09 U/mL was achieved, which is less than the HAase level found in normal human body fluids. Moreover, this assay may be used for diagnosing HAase-related diseases.

scholarly journals A new ratiometric fluorescence assay based on resonance energy transfer between biomass quantum dots and organic dye for the detection of sulfur dioxide derivatives

RSC Advances ◽  
2019 ◽  
Vol 9 (71) ◽  
pp. 41955-41961 ◽  
Author(s):  
Jingjin Zhao ◽  
Yao Peng ◽  
Keqin Yang ◽  
Yunyun Chen ◽  
Shulin Zhao ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Sulfur Dioxide ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Organic Dye ◽  
Fluorescence Assay ◽  
Ratiometric Fluorescence ◽  
Cell Lysates

A new ratiometric fluorescence assay has been developed for the detection of sulfur dioxide derivatives with repeatability and selectivity. The assay was applied to quantitate HSO3− in cell lysates with accurate results.

Tuning Optical Properties of Nitrogen-Doped Carbon Dots Through Fluorescence Resonance Energy Transfer Using Rhodamine B for Ratiometric Sensing of Mercury ion

2021 ◽  
Author(s):  
Alagan Muthurasu ◽  
V GANESH
Keyword(s):  
Optical Properties ◽  
Energy Transfer ◽  
Rhodamine B ◽  
Carbon Dots ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Nitrogen Doped ◽  
Physical Chemical ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

Carbon dots (CDs) exhibiting fluorescence property are generally derived from carbonaceous materials and possessing ultra small size with various exciting physical, chemical and photo-properties that have been used in many...

Fluorescence resonance energy transfer monitoring of pH-responsive doxorubicin release from carbon dots/aptamer functionalized magnetic mesoporous silica

Nanomedicine ◽  
2021 ◽  
Author(s):  
Abolghasem Abbasi Kajani ◽  
Masoud Ayatollahi Mehrgardi
Keyword(s):  
Drug Delivery ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Carbon Dots ◽  
Drug Loading ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Spectroscopic Techniques ◽  
Ph Responsive ◽  
Fluorescence Resonance

Aim: To develop a novel theranostic nanoplatform for simultaneous fluorescent monitoring and stimuli-triggered drug delivery. Materials & methods: Different microscopic and spectroscopic techniques were used for the characterization of nanocarriers. MCF-7 and human umbilical vein endothelial cell lines were cultured and treated with different doses of doxorubicin-loaded nanocarriers. The cell viability and drug release were studied using MTT assay and fluorescence microscopy. Results: Biocompatible and mono-disperse nanocarriers represent hollow and mesoporous structures with the calculated surface area of 552.83 m2.g-1, high magnetic activity (12.6 emu.g-1), appropriate colloidal stability and high drug loading capacity (up to 61%). Conclusion: Taxane-based carbon dots act as the pH-responsive gatekeepers for the controlled release of doxorubicin into cancer cells and provide a fluorescence resonance energy transfer system for real-time monitoring of drug delivery.

scholarly journals Self-illumination of Carbon Dots by Bioluminescence Resonance Energy Transfer

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jisu Song ◽  
Jin Zhang
Keyword(s):  
Energy Transfer ◽  
Light Source ◽  
High Power ◽  
Carbon Dots ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fluorescent Nanoparticles ◽  
Energy Transfer Mechanism ◽  
Bioluminescence Resonance Energy Transfer ◽  
Relationship Of

Abstract Carbon-dots (CDs), the emerging fluorescent nanoparticles, show special multicolor properties, chemical stability, and biocompatibility, and are considered as the new and advanced imaging probe in replacement of molecular fluorophores and semiconductor quantum dots. However, the requirement of external high power light source limits the application of fluorescent nanomaterials in bio-imaging. The present study aims to take advantage of bioluminescence resonance energy transfer mechanism (BRET) in creating self-illuminating C-dots. Renilla luciferase (Rluc) is chosen as the BRET donor molecule. Conjugation of Renilla luciferase and C-dots is necessary to keep their distance close for energy transfer. The optimal condition for achieving BRET is investigated by studying the effects of different factors on the performance of BRET, including the type of conjugation, concentration of carbon dots, and conjugation time. The linear relationship of BRET efficiency as a function of the amount of C-dots in the range of 0.20–0.80 mg/mL is observed. The self-illuminating carbon dots could be applied in bioimaging avoiding the tissue damage from the external high power light source.

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