Biomimetic honeycomb-patterned surface as the tunable cell adhesion scaffold

2015 ◽  
Vol 3 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Shuangshuang Chen ◽  
Xuemin Lu ◽  
Ying Hu ◽  
Qinghua Lu
Keyword(s):  
Cell Adhesion ◽  
Dip Coating ◽  
Fish Skin ◽  
Structured Surfaces ◽  
Patterned Surface ◽  
Parthenocissus Tricuspidata

PS honeycomb structured surfaces were modified into both cell-philic and cell-phobic by dip-coating and casting polySBMA, respectively, which was inspired by two typically adhesive behaviours of fish skin and Parthenocissus tricuspidata.

scholarly journals Surface functionalization through sol-gel dip coating for thiol-mediated cell adhesion

2016 ◽  
Vol 4 ◽  
Author(s):  
Altomare Lina ◽  
Pezzoli Daniele ◽  
Tana Francesca ◽  
De Nardo Luigi ◽  
Candiani Gabriele
Keyword(s):  
Cell Adhesion ◽  
Surface Functionalization ◽  
Sol Gel ◽  
Dip Coating

scholarly journals Surface Structuring Combined with Chemical Surface Functionalization: An Effective Tool to Manipulate Cell Adhesion

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 909 ◽  
Author(s):  
Sarah Elsayed ◽  
Stefan Paschke ◽  
Sibylle Rau ◽  
Karen Lienkamp
Keyword(s):  
Antimicrobial Activity ◽  
Cell Adhesion ◽  
Surface Structure ◽  
Resonance Spectroscopy ◽  
Structured Surfaces ◽  
Chemical Surface ◽  
Force Microscopy ◽  
Tissue Integration ◽  
Protein Adhesion ◽  
Escherichia Coli Bacteria

In this study, we investigate how a surface structure underneath a surface-attached polymer coating affects the bioactivity of the resulting material. To that end, structured surfaces were fabricated using colloidal lithography (lateral dimensions: 200 nm to 1 µm, height ~15 to 50 nm). The surface structures were further functionalized either with antimicrobial, cell-adhesive polycations or with protein-repellent polyzwitterions. The materials thus obtained were compared to non-functionalized structured surfaces and unstructured polymer monolayers. Their physical properties were studied by contact-angle measurements and atomic force microscopy (AFM). Protein adhesion was studied by surface plasmon resonance spectroscopy, and the antimicrobial activity against Escherichia coli bacteria was tested. The growth of human mucosal gingiva keratinocytes on the materials was analyzed using the Alamar blue assay, optical microscopy, and live-dead staining. The data shows that the underlying surface structure itself reduced protein adhesion and also bacterial adhesion, as evidenced by increased antimicrobial activity. It also enhanced cell adhesion to the surfaces. Particularly in combination with the adhesive polycations, the surfaces increased the cell growth compared to the unstructured reference materials. Thus, functionalizing structured surfaces with adhesive polymer could be a valuable tool for improved tissue integration.

scholarly journals Tunable Hierarchical Nanostructures on Micro-Conical Arrays of Laser Textured TC4 Substrate by Hydrothermal Treatment for Enhanced Anti-Icing Property

Coatings ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 450
Author(s):  
Mengyao Liu ◽  
Rui Zhou ◽  
Zhekun Chen ◽  
Huangping Yan ◽  
Jingqin Cui ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Hydrothermal Treatment ◽  
Hierarchical Structures ◽  
Contact Angles ◽  
Hydrothermal Temperature ◽  
Hierarchical Nanostructures ◽  
Structured Surfaces ◽  
Patterned Surface ◽  
Nanosecond Pulsed Laser ◽  
Tio2 Nanostructures

In this work, an anti-icing structured surface was fabricated by combining laser ablation with hydrothermal treatment. A micro-patterned surface on a Ti alloy (TC4) substrate was easily fabricated by a highly effective nanosecond pulsed laser ablation. It was observed that titania (TiO2) nanostructures were formed by hydrothermal treatment in aqueous alkali on the laser ablated TC4 substrate to obtain the micro/nano-hierarchical structures. The growth mechanism of the tunable nanoarrays was discussed by the adjustment of hydrothermal temperature. The as-prepared samples exhibited excellent superhydrophobicity with contact angles greater than 160°. It was found that optimized hydrothermal treatment on laser-processed TC4 substrates could further enhance surface anti-icing property. The results showed that the delay time (DT) had been extended by achieving over 90 min for the water droplets to freeze on the as-prepared structured surfaces, providing great potential in various anti-icing applications.

scholarly journals Femtosecond Laser Nano/Micro Textured Ti6Al4V Surfaces—Effect on Wetting and MG-63 Cell Adhesion

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2210 ◽  
Author(s):  
Georg Schnell ◽  
Susanne Staehlke ◽  
Ulrike Duenow ◽  
J. Barbara Nebe ◽  
Hermann Seitz
Keyword(s):  
Cell Adhesion ◽  
Surface Properties ◽  
Laser Scanning ◽  
Surface Characterization ◽  
Cellular Adhesion ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Material Surface ◽  
Structured Surfaces

Nano- and microstructured titanium surfaces have recently attracted attention in the field of regenerative medicine because of the influence which surface characteristics such as roughness and wettability can have on cellular processes. This study focuses on the correlation of surface properties (wettability and nano/micro texture) of laser-structured Ti6Al4V samples with pronounced cell adhesion. Samples were structured with multiple laser parameters in order to create a range of surface properties. Surface characterization was performed by contact angle measurements 1 and 7 days after laser processing. The arithmetic mean roughness of the material surface in an area (Sa) was determined by means of confocal laser scanning microscopy (CLSM). Immediately after wettability tests of the laser-structured surfaces, in vitro experiments with human MG-63 osteoblasts were carried out. For this purpose, the cell morphology and actin cytoskeleton organization were analyzed using CLSM and scanning electron microscopy. On rough microstructures with deep cavities, the cell growth and spreading were inhibited. An improved cellular adhesion and growth on nanostructured and sinusoidal microstructured surfaces could be demonstrated, regardless of hydrophilicity of the surfaces.

Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation

2014 ◽  
Vol 1621 ◽  
pp. 107-112
Author(s):  
Yoshikazu Kumashiro ◽  
Jun Ishihara ◽  
Terumasa Umemoto ◽  
Kazuyoshi Itoga ◽  
Jun Kobayashi ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Umbilical Vein ◽  
Free Cell ◽  
Specific Cell ◽  
Label Free ◽  
Stripe Pattern ◽  
Adhesion Properties ◽  
Patterned Surface ◽  
Umbilical Vein Endothelial Cells ◽  
Response To Temperature

ABSTRACTThready stripe-patterned thermo-responsive surfaces were prepared and their surface properties were characterized. Prepared 3 μm wide stripe-patterned surfaces were evaluated by observing the adhesions and detachments of three types of cells: HeLa cells (HeLas), human umbilical vein endothelial cells (HUVECs), and NIH-3T3 cells (3T3s). Although cell adhesion and detachment in response to temperature were observed on all cells on a conventional thermo-responsive surface without patterns, the thermo-responsive surface with a 3 μm striped-pattern exhibited various cell adhesion properties. HeLas hardly adhered to the patterned surface even at 37 °C. On the other hand, although HUVECs adhered on the patterned surface at 12 h after incubation at 37 °C, the adhered HUVECs detached themselves after another 12 h incubation at 37 °C. 3T3s adhered to the patterned surface at 37 °C and detached themselves after reducing temperature to 20 °C. A mixture of HeLa, HUVEC and 3T3 was separated using their different specific cell-adhesion properties, and the composition of cells was analyzed by a flow-cytometry. As a result, the conventional thermo-responsive surface with a stripe-pattern was found to function as a cell-separating interface by using specific cell adhesion properties.

Modulation of neo-endothelialization of vascular graft materials by silk fibroin

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Congcong Zhan ◽  
Chuanjun Xia ◽  
Pengfei Wang ◽  
Pingdeng Ming ◽  
Shanfeng Zhang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Adhesion ◽  
Silk Fibroin ◽  
Vascular Graft ◽  
Vascular Grafts ◽  
Dip Coating ◽  
Coating Process ◽  
Quantitative Analyse ◽  
Scanning Electron

Abstract Controlled neo-endothelialization is critical to the patency of vascular grafts. Expanded polyethylene terephthalate (PET) vascular grafts were grafted with polyethylene glycol (PEG), irradiated with ultraviolet light, and subsequently coated with silk fibroin (SF) and EDC in a dip-coating process. Endothelial cells were cultivated on the coated samples for 1, 3, 5, and 7 days, and characterized by fluorescence microscopy and scanning electron microscopy (SEM). The quantitative analyse of CCK-8 method was used to assess ECs proliferation. The results reveal the correlation between grafting components and cell adhesion. We demonstrated that PET with SF grafting facilitated cell adhesion and spreading. Following 7 days of cell culture in vitro, PET-PEG6000-SF (PEG molecular weight 6,000) displayed spreading of cells over a significantly larger area. Rapid endothelialization on a modified PET surface resulted in large tissue pack that can be observed by SEM.

Study on Degradation Behavior and Biocompatibility of Polymethyl Methacrylate/Mineralized Collagen/Mg–Ca Alloy Composite Material

2020 ◽  
Vol 10 (2) ◽  
pp. 139-150
Author(s):  
Yuan-Xin Shen ◽  
Xi-Rao Sun ◽  
Cheng-Yue Wang ◽  
Jing-Xin Yang ◽  
Jia-Xin Bao
Keyword(s):  
Weight Loss ◽  
Alkaline Phosphatase ◽  
Cell Adhesion ◽  
Orthopedic Implants ◽  
Dip Coating ◽  
Alizarin Red ◽  
Pmma Bone Cement ◽  
Degradation Test ◽  
Mineralized Collagen

In this study, we composited mineralized collagen and magnesium-calcium alloy by freeze-drying, followed by dip-coating PMMA bone cement to enhance the composite of mineralized collagen and magnesium-calcium alloy. In vitro degradation test was performed to observe the pH and weight loss of the material. The contact angle test was used to detect the hydrophilicity of the material. Subsequently, MC3T3-E1 were used to assess cell biocompatibility In vitro by cell adhesion, cytotoxicity, alkaline phosphatase, alizarin red staining, and cytoskeleton. The results showed that the pH changes of the PMMA/NHAC/Mg–Ca was slower than that of the Mg–Ca , and the weight loss rate at 7 d and 14 d were lower than that of the Mg–Ca (P < 0.05) in degradation test. Wettability experiment showed that PMMA/NHAC/Mg–Ca was a hydrophilic material and Mg–Ca was a hydrophobic material (P < 0.05). In vitro cell experiments, the PMMA/NHAC/Mg–Ca had more cell adhesion than Mg–Ca and more synapses were connected to others. In the cytotoxicity experiment, the cell proliferation lever of PMMA/NHAC/Mg–Ca was higher than that of Mg–Ca at each time point (P < 0.05). In the 7 d alkaline phosphatase experiment, the PMMA/NHAC/Mg–Ca showed higher ALP activity than the Mg–Ca (P < 0.05), and in the alizarin red experiment at 14 d and 28 d, there were more obvious calcified nodules and mineralized area. After 1 day of culture in the PMMA/NHAC/Mg–Ca extract, the cells showed a clearer and more complete cytoskeletal structure and better cell morphology. In conclusion, PMMA/NHAC/Mg–Ca orthopedic implants had a better hydrophilicity, cytotoxicity and osteogenic ability, besides with a slower rate of degradation, and could be implanted in animals for further research, which were expected to be used for the repair of clinical bone defects.

scholarly journals Inclusion of gold nanoparticles in meso-porous silicon for the SERS analysis of cell adhesion on nano-structured surfaces

2016 ◽  
Vol 158 ◽  
pp. 102-106 ◽  
Author(s):  
M.L. Coluccio ◽  
S. De Vitis ◽  
G. Strumbo ◽  
P. Candeloro ◽  
G. Perozziello ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Adhesion ◽  
Porous Silicon ◽  
Structured Surfaces

An immunocytochemical study of maternal immunoglobulin localization in the suckling pig intestine

1990 ◽  
Vol 48 (3) ◽  
pp. 922-923
Author(s):  
László G. Kömüves ◽  
Donna S. Turner ◽  
Kathy S. McKee ◽  
Buford L. Nichols ◽  
Julian P. Heath
Keyword(s):  
Sodium Ethoxide ◽  
Intracellular Localization ◽  
Protein A ◽  
Tween 20 ◽  
Intestinal Epithelial ◽  
Immunocytochemical Study ◽  
Fish Skin ◽  
Newborn Piglets ◽  
Rabbit Igg ◽  
Fish Skin Gelatin

In this study we used colloidal gold probes to detect the intracellular localization of colostral immunoglobulins in intestinal epithelial cells of newborn piglets.Tissues were obtained from non-suckled newborn and suckled piglets aged between 1 hour to 1 month. Samples were fixed in 2.5 % glutaraldehyde, osmicated and embedded into Spurr’s resin. Thin (80 nm) sections were etched with 5% sodium ethoxide for 5 min, washed and treated with 4 % sodium-m-periodate in distilled water for 30 min. The sections were then first incubated with blocking buffer (2 % BSA, 0.25 % fish skin gelatin, 0.5 % Tween 20 in 10 mM Trizma buffer, pH=7.4 containing 500 mM NaCl) for 30 min followed by the immunoreagents diluted in the same buffer, 1 hr each. For the detection of pig immunoglobulins a rabbit anti-pig IgG antiserum was used followed by goat anti-rabbit IgG-Au10 or protein A-Au15 probes.

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