A label-free electrochemical aptasensor for the detection of kanamycin in milk

2015 ◽  
Vol 7 (5) ◽  
pp. 1991-1996 ◽  
Author(s):  
Nandi Zhou ◽  
Jibao Luo ◽  
Juan Zhang ◽  
Yuanding You ◽  
Yaping Tian
Keyword(s):  
Conformational Change ◽  
Electrode Surface ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Current Change

Kanamycin is detected based on the conformational change of the aptamer attached to the electrode surface and the corresponding SWV current change in [Fe(CN)6]3−/4−solution.

scholarly journals Electrochemical Aptasensors: Current Status and Future Perspectives

Diagnostics ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 104
Author(s):  
Ragaa Abd-Ellatief ◽  
Maha Ragaa Abd-Ellatief
Keyword(s):  
Conformational Change ◽  
Electrode Surface ◽  
Current Status ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Future Perspectives ◽  
Target Analytes ◽  
Target Binding ◽  
Competition Assays

This article reviews the progress of diversity of electrochemical aptasensor for target analytes detection. The immobilization strategies of aptamers on an electrode surface are addressed. The aptasensors are also introduced in compliance with the assay platforms. Many electrochemical aptasensors are nearly identical to conventional immunochemical approaches, sandwich and competition assays using electroactive signaling moieties. Others are “signal-on” and “sign-off” aptasensors credited to the target binding-induced conformational change of aptamers. Label-free aptasensors are also highlighted. Furthermore, the aptasensors applied for clinically important biomarkers are emphasized.

Gold nanoparticles/Orange II functionalized graphene nanohybrid based electrochemical aptasensor for label-free determination of insulin

RSC Advances ◽  
2016 ◽  
Vol 6 (36) ◽  
pp. 30732-30738 ◽  
Author(s):  
Tingting Li ◽  
Zhiguang Liu ◽  
Li Wang ◽  
Yujing Guo
Keyword(s):  
Gold Nanoparticles ◽  
Electrode Surface ◽  
Insulin Binding ◽  
Orange Ii ◽  
Functionalized Graphene ◽  
Label Free ◽  
Electrochemical Aptasensor

Nanocomposites, gold nanoparticles on Orange II functionalized graphene (AuNPs/O-GNs), were developed to modify the electrode surface for anchoring an insulin binding aptamer.

Fabrication of electro-active nano-trans surfaces to design label free electrochemical aptasensor for ochratoxin A detection

2021 ◽  
Vol 379 ◽  
pp. 138172
Author(s):  
Maham Liaqat ◽  
Sara Riaz ◽  
Mian Hasnain Nawaz ◽  
Mihaela Badea ◽  
Akhtar Hayat ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Label Free ◽  
Electrochemical Aptasensor

A label-free electrochemical aptasensor based on the core–shell Cu-MOF@TpBD hybrid nanoarchitecture for the sensitive detection of PDGF-BB

The Analyst ◽  
2021 ◽  
Author(s):  
Ya Li ◽  
Zhiling Liu ◽  
Wenbo Lu ◽  
Man Zhao ◽  
He Xiao ◽  
...  
Keyword(s):  
Hybrid Nanocomposites ◽  
Sensitive Detection ◽  
Core Shell ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
The Core

A novel label-free electrochemical aptasensor based on core–shell Cu-MOF@TpBD hybrid nanocomposites has been prepared for the sensitive detection of PDGF-BB.

Boolean Logic Tree of Label-Free Dual-Signal Electrochemical Aptasensor System for Biosensing, Three-State Logic Computation, and Keypad Lock Security Operation

2017 ◽  
Vol 89 (18) ◽  
pp. 9734-9741 ◽  
Author(s):  
Jiao Yang Lu ◽  
Xin Xing Zhang ◽  
Wei Tao Huang ◽  
Qiu Yan Zhu ◽  
Xue Zhi Ding ◽  
...  
Keyword(s):  
Boolean Logic ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Logic Tree ◽  
Logic Computation

Encapsulation of hemin in Fe-based metal-organic frameworks and its application for the direct determination of aflatoxin M1

2021 ◽  
pp. 1-10
Author(s):  
F. Jahangiri-Dehaghani ◽  
H.R. Zare ◽  
Z. Shekari
Keyword(s):  
Electrochemical Impedance ◽  
Metal Organic Frameworks ◽  
Direct Determination ◽  
Differential Pulse ◽  
Aflatoxin M1 ◽  
Label Free ◽  
Electrochemical Aptasensor ◽  
Selective Determination ◽  
Metal Organic

A label-free electrochemical aptasensor was constructed for the sensitive and selective determination of AFM1. For preparation of the aptasensor, the AFM1 aptamer was immobilised on the surface of a glassy carbon electrode modified with hemin encapsulated in Fe-based metal-organic frameworks (hemin@Fe-MIL-101). The morphology and the structure of Fe-MIL-101 and hemin@Fe-MIL-101 were evaluated by scanning electron microscopy, Fourier-transform infrared spectroscopy, X-ray powder diffraction and Brunauer-Emmett-Teller-N2 sorption methods. Electrochemical impedance spectroscopy and cyclic voltammetry were performed to monitor the fabrication process of the electrochemical aptasensor. The electrochemical reduction current of hemin encapsulated in Fe-MIL-101 serves as a signal for the quantitative determination of AFM1. Differential pulse voltammetry was done to determine the AFM1 concentration in the linear range of 1.0×10-1-100.0 ng/ml. The detection limit of AFM1 was estimated to be 4.6×10-2 ng/ml. Finally, the fabricated aptasensor was applied to determine AFM1 in raw and boiled milk samples.

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