Colourimetric detection of Ag(i) ions using dCTP-stabilised gold nanoparticles

2015 ◽  
Vol 7 (3) ◽  
pp. 1110-1114 ◽  
Author(s):  
Chun-Guang Yang ◽  
Miao Zhang ◽  
Zhang-Run Xu

A simple colourimetric detection method for Ag(i) ions based on dCTP-stabilised gold nanoparticles is proposed.

2017 ◽  
Vol 9 (33) ◽  
pp. 4843-4850 ◽  
Author(s):  
Chunshuai Wang ◽  
Jing Liu ◽  
Xuyan Han ◽  
Chang Liu ◽  
Yaping Tian ◽  
...  

A UV-visible spectroscopic detection method of kanamycin was successfully developed based on target-induced growth of gold nanoparticles (AuNPs), using AuNPs as the probe and a kanamycin-specific aptamer as the recognition element.


2019 ◽  
Vol 123 ◽  
pp. 19-24 ◽  
Author(s):  
Dan Zhang ◽  
Shuyuan Du ◽  
Shupeng Su ◽  
Ying Wang ◽  
Hongyan Zhang

2014 ◽  
Vol 6 (13) ◽  
pp. 4507-4511 ◽  
Author(s):  
Dinggui Dai ◽  
Dong Xu ◽  
Xiaodong Cheng ◽  
Yan He

We present a highly sensitive Pb2+ detection method by in situ real-time imaging of Pb2+ catalyzed etching and size reduction of immobilized single gold nanoparticles with darkfield microscopy.


2007 ◽  
Vol 7 (2) ◽  
pp. 712-716 ◽  
Author(s):  
Wenrong Yang ◽  
J. Justin Gooding ◽  
Zhicong He ◽  
Qiong Li ◽  
Guonan Chen

This communication reports an efficient visual detection method of Cu2+ by L-cysteine functionalized gold nanoparticles in aqueous solution. Upon exposure to Cu2+, the gold nanoparticle solution changed from red to blue, in response to surface plasmon absorption of dispersed and aggregated nanoparticles. This colorimetric sensor allows a rapid quantitative assay of Cu2+ down to the concentration range of 10−5 M. Recognition of Cu2+ and formation of the aggregates are proposed to occur via a 2 : 1 sandwich complex between L-cysteine and Cu2+.


2020 ◽  
Vol 44 (37) ◽  
pp. 15828-15835
Author(s):  
Shuaihui Feng ◽  
Rongjia Shi ◽  
Ping Xu ◽  
Jigna R. Bhamore ◽  
Jyotiranjan Bal ◽  
...  

A new selective detection method was developed for creatinine by combining gold nanoparticles (GNPs) and peptide probes which were screened and selected using phage-display technology.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
R. Lavanya ◽  
V. Arun

AbstractBegomoviruses are a major class of Geminiviruses that affects most dicotyledonous plants and causes heavy economic losses to farmers. Early detection of begomovirus is essential to control the spread of the disease and prevent loss. Many available detection methods like ELISA, immunosorbent electron microscopy, PCR or qPCR require expertise in handling sophisticated instruments, complex data interpretation and costlier chemicals, enzymes or antibodies. Hence there is a need for a simpler detection method, here we report the development of a visual detection method based on functionalized gold nanoparticles (AuNP assay). The assay was able to detect up to 500 ag/µl of begomoviral DNA (pTZCCPp3, a clone carrying partial coat protein gene) suspended in MilliQ water. Screening of chilli plants for begomoviral infection by PCR (Deng primers) and AuNP assay showed that AuNP assay (77.7%) was better than PCR (49.4%). The AuNP assay with clccpi1 probe was able to detect begomoviral infection in chilli, tomato, common bean, green gram and black gram plants which proved the utility and versatility of the AuNP assay. The specificity of the assay was demonstrated by testing with total DNA from different plants that are not affected by begomoviruses.


2021 ◽  
Author(s):  
Lavanya Ramesh ◽  
Arun Viswanathan

Abstract Begomoviruses are a major class of Geminiviruses that affects most dicotyledonous plants and causes heavy economic losses to farmers. Early detection of begomovirus is essential to control the spread of the disease and prevent loss. Many available detection methods like ELISA, immunosorbent electron microscopy, PCR or qPCR require expertise in handling sophisticated instruments, complex data interpretation and costlier chemicals, enzymes or antibodies. Hence there is a need for a simpler detection method, here we report the development of a visual detection method based on functionalized gold nanoparticles (AuNP probe assay). The assay was able to detect upto 500 ag/𝜇l of begomoviral DNA (pTZCCPp3, a clone carrying partial coat protein gene) suspended in MilliQ water. Screening of chilli plants for begomoviral infection by PCR (Deng primers) and AuNP assay showed that AuNP assay (77.7%) was better than PCR (49.4%). The AuNP assay with clccpi1 probe was able to detect begomoviral infection in chilli, tomato, common bean, green gram and black gram plants which proved the utility and versatility of the AuNP assay. The specificity of the assay was demonstrated by testing with total DNA from different plants that are not affected by begomoviruses.


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