Facile colorimetric detection of human chorionic gonadotropin based on the peptide-induced aggregation of gold nanoparticles

2015 ◽  
Vol 7 (1) ◽  
pp. 29-33 ◽  
Author(s):  
Chia-Chen Chang ◽  
Chen-Yu Chen ◽  
Chie-Pein Chen ◽  
Chii-Wann Lin
Keyword(s):  
Gold Nanoparticles ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Detection Method ◽  
Colorimetric Detection ◽  
Optical Detection ◽  
Binding Peptide ◽  
Induced Aggregation

A facile and rapid optical detection method of human chorionic gonadotropin (hCG) was developed by using the hCG-binding peptide and the anti-aggregation of gold nanoparticles (AuNPs).

Colorimetric detection of human chorionic gonadotropin using catalytic gold nanoparticles and a peptide aptamer

2014 ◽  
Vol 50 (92) ◽  
pp. 14443-14446 ◽  
Author(s):  
Chia-Chen Chang ◽  
Chie-Pein Chen ◽  
Chung-Han Lee ◽  
Chen-Yu Chen ◽  
Chii-Wann Lin
Keyword(s):  
Gold Nanoparticles ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Colorimetric Detection ◽  
Peptide Aptamer

A highly sensitive gold nanoparticle bioprobe based electrochemical immunosensor using screen printed graphene biochip

RSC Advances ◽  
2014 ◽  
Vol 4 (102) ◽  
pp. 58460-58466 ◽  
Author(s):  
Syazana Abdullah Lim ◽  
Hiroyuki Yoshikawa ◽  
Eiichi Tamiya ◽  
Hartini Mohd Yasin ◽  
Minhaz Uddin Ahmed
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Electrode Material ◽  
Electrochemical Immunosensor ◽  
Highly Sensitive ◽  
Electrochemical Label ◽  
Screen Printed

This study describes a highly sensitive electrochemical immunosensor for the detection of human chorionic gonadotropin (hCG) that uses gold nanoparticles (AuNP) as the electrochemical label and graphene as electrode material.

Chitosan-functionalized gold nanoparticles for colorimetric detection of mercury ions based on chelation-induced aggregation

2014 ◽  
Vol 182 (3-4) ◽  
pp. 611-616 ◽  
Author(s):  
Zhengbo Chen ◽  
Chenmeng Zhang ◽  
Yuan Tan ◽  
Tianhui Zhou ◽  
He Ma ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Mercury Ions ◽  
Functionalized Gold Nanoparticles ◽  
Induced Aggregation

Importance of the detection method for intact dimeric human chorionic gonadotropin without interference with the free human chorionic gonadotropin β subunit for pregnancy exclusion before liver transplantation in a woman with cholangiocarcinoma

2005 ◽  
Vol 43 (8) ◽  
Author(s):  
Catherine Massart ◽  
Catherine Lucas ◽  
Nathalie Rioux-Leclercq ◽  
Patricia Fergelot ◽  
Véronique Pouvreau-Quillien ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Detection Method ◽  
Gastrointestinal Cancers ◽  
Cell Secretion ◽  
Specific Serum ◽  
Trophoblastic Tumors ◽  
Β Hcg

AbstractAssay of human chorionic gonadotropin (hCG) is mainly used for the detection and monitoring of pregnancy, and for the follow-up of trophoblastic tumors. The serum free β-hCG subunit (hCGβ) is also a tumor marker in many non-trophoblastic tumors, including gastrointestinal cancers. In this work, we compared the performance of several immunoassays for pregnancy exclusion before liver transplantation and in the follow-up of a woman with cholangiocarcinoma. Serum hCG was detected with the Abbott Testpack plus hCG-Combo and measured with four automated sandwich immunoassays: ADVIA-Centaur, ACS:180, AxSYM and Dimension. hCGβ was determined by an automated fluorescence sandwich immunoassay (Kryptor-Free β hCG) and with a specific immunoradiometric assay (ELSA-F β hCG, Schering). The expression of hCG was also evaluated by immunohistochemistry on sections of intrahepatic cholangiocarcinoma cells and on peritoneal metastases. Before transplantation, discordant results were observed for pregnancy exclusion. Qualitative Testpack and Dimension tests detected no hCG-like immunoreactivity, whereas the ADVIA-Centaur, ACS:180 and AxSYM tests revealed positive levels. The same discrepancy was obtained in follow-up of the patient after liver transplantation. hCGβ assay and immunohistochemical staining revealed tumor cell secretion of hCGβ. In conclusion, a specific serum immunoassay for intact dimeric hCG without cross-reaction with hCGβ should be adopted as routine policy for pregnancy exclusion before liver transplantation.

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