UPLC-Q-TOF-MS/MS fingerprinting for rapid identification of the chemical constituents of Ermiao Wan

2015 ◽  
Vol 7 (3) ◽  
pp. 846-862 ◽  
Author(s):  
Guangli Yan ◽  
Di Zou ◽  
Aihua Zhang ◽  
Yunlong Tan ◽  
Hui Sun ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Rapid Identification ◽  
Determination Method ◽  
Tof Ms

In this work, a novel and efficient determination method for the rapid identification of multi-class chemical constituents of Ermiao Wan has been developed.

RRLC-TOF/MS in rapid identification of 43 chemical constituents of epimedium

2011 ◽  
Vol 31 (3) ◽  
pp. 306-310 ◽  
Author(s):  
Lei LU ◽  
Hai ZHANG ◽  
Liang ZHAO ◽  
Jing JIA ◽  
Yue-yue LI ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Rapid Identification ◽  
Tof Ms

HPLC-TOF/MS in identification of chemical constituents of Fuzhengpingxiao Capsule

2013 ◽  
Vol 32 (7) ◽  
pp. 770-779
Author(s):  
Liang ZHAO ◽  
Wen-jun TIAN ◽  
Lei LU ◽  
Hai ZHANG ◽  
Xin-xia WANG ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Tof Ms

scholarly journals Rapid identification of direct-acting pancreatic protectants from Cyclocarya paliurus leaves tea by the method of serum pharmacochemistry combined with target cell extraction

2020 ◽  
Vol 18 (1) ◽  
pp. 265-274
Author(s):  
Wei-hong Chen ◽  
Zhen Luo ◽  
Zi-Wan Ning ◽  
Jiao Peng ◽  
Xiao-peng Hu ◽  
...  
Keyword(s):  
Target Cell ◽  
Rapid Identification ◽  
Target Cells ◽  
Bioactive Components ◽  
Β Cell ◽  
Cyclocarya Paliurus ◽  
Cell Extraction ◽  
Direct Acting ◽  
Apoptotic Effects ◽  
Tof Ms

AbstractExtracts of Cyclocarya paliurus (CP) leaves, a popular sweet tea, inhibit pancreatic β cell apoptosis and have potent hypoglycemic effects, but the identities of the anti-apoptotic bioactive components are still unknown. In the present study, a method using UPLC-Q-TOF/MS based on serum pharmacochemistry combined with target cell extraction was established to rapidly identify direct-acting pancreatic protectants from CP. After orally administering a set amount of CP extract to rats, blood samples were collected to characterize the components that can be absorbed into the blood using UPLC-Q-TOF/MS. Also, target cells (pancreatic β NIT-1 cells) were incubated with CP extract for 24 hours, and cells were collected to identify the components that can bind to the cells using UPLC-Q-TOF/MS. Finally, to evaluate the protective effect of the bioactive components of CP, MTT and TUNEL assays were performed on treated NIT-1 cell induced by streptozotocin (STZ). Three potential direct-acting pancreatic protectants -- kaempferol, quercetin, quadranoside IV -- were identified, and anti-apoptotic effects of kaempferol and quercetin were confirmed in STZ-induced NIT-1 cells. The findings indicate that this combined approach is a feasible, rapid, and expedient tool for capturing potential direct-acting components from natural products such as those from CP leaves.

scholarly journals Chemical Fingerprinting of Cryptic Species and Genetic Lineages of Aneura pinguis (L.) Dumort. (Marchantiophyta, Metzgeriidae)

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1180
Author(s):  
Rafał Wawrzyniak ◽  
Wiesław Wasiak ◽  
Beata Jasiewicz ◽  
Alina Bączkiewicz ◽  
Katarzyna Buczkowska
Keyword(s):  
Cluster Analysis ◽  
Cryptic Species ◽  
Plant Material ◽  
Chemical Constituents ◽  
Principal Component ◽  
Molecular Data ◽  
Rapid Identification ◽  
Multivariate Statistical ◽  
Genetic Lineages ◽  
Marker Compounds

Aneura pinguis (L.) Dumort. is a representative of the simple thalloid liverworts, one of the three main types of liverwort gametophytes. According to classical taxonomy, A. pinguis represents one morphologically variable species; however, genetic data reveal that this species is a complex consisting of 10 cryptic species (named by letters from A to J), of which four are further subdivided into two or three evolutionary lineages. The objective of this work was to develop an efficient method for the characterisation of plant material using marker compounds. The volatile chemical constituents of cryptic species within the liverwort A. pinguis were analysed by GC-MS. The compounds were isolated from plant material using the HS-SPME technique. Of the 66 compounds examined, 40 were identified. Of these 40 compounds, nine were selected for use as marker compounds of individual cryptic species of A. pinguis. A guide was then developed that clarified how these markers could be used for the rapid identification of the genetic lineages of A. pinguis. Multivariate statistical analyses (principal component and cluster analysis) revealed that the chemical compounds in A. pinguis made it possible to distinguish individual cryptic species (including genetic lineages), with the exception of cryptic species G and H. The classification of samples based on the volatile compounds by cluster analysis reflected phylogenetic relationships between cryptic species and genetic lineages of A. pinguis revealed based on molecular data.

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