Rapid identification and comparative analysis of the chemical constituents and metabolites ofPhellodendri amurensiscortex and Zhibai dihuang pill by ultra-performance liquid chromatography with quadrupole TOF-MS

2013 ◽  
Vol 36 (24) ◽  
pp. 3874-3882 ◽  
Author(s):  
Huiyu Wang ◽  
Hui Sun ◽  
Aihua Zhang ◽  
Yuan Li ◽  
Lihong Wang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Comparative Analysis ◽  
Chemical Constituents ◽  
Ultra Performance Liquid Chromatography ◽  
Rapid Identification ◽  
Tof Ms

Comparative analysis of the complete chloroplast genomic sequence and chemical components of Cinnamomum micranthum and Cinnamomum kanehirae

Holzforschung ◽  
2017 ◽  
Vol 71 (3) ◽  
pp. 189-197 ◽  
Author(s):  
Chia-Chen Wu ◽  
Fang-Hua Chu ◽  
Cheng-Kuen Ho ◽  
Chia-Hsuan Sung ◽  
Shu-Hwa Chang
Keyword(s):  
Phylogenetic Analysis ◽  
Liquid Chromatography ◽  
Comparative Analysis ◽  
Chloroplast Genome ◽  
Genomic Sequence ◽  
Ultra Performance Liquid Chromatography ◽  
Chemical Components ◽  
Complete Chloroplast Genome ◽  
Medicinal Fungus ◽  
Indel Markers

Abstract Cinnamomum micranthum (Hayata) Hayata is a tree species that is often confused with Cinnamomum kanehirae Hayata, which is an endemic species in Taiwan and the sole natural host of the valuable medicinal fungus Antrodia cinnamomea. However, the two species are highly similar in morphology and difficult to distinguish based on traditional vegetative structures or wood anatomical methods. The aim of the present study was to identify of these two species by DNA and chemical analysis. The complete chloroplast genome of C. micranthum has been determined and compared with that of C. kanehirae. The leaf methanol extracts of these species were also analyzed by ultra-performance liquid chromatography (UPLC). The length of the C. micranthum chloroplast genome was 152675 bp, i.e. 25 bp shorter than that of C. kanehirae. In phylogenetic analysis, C. micranthum was more closely related to C. kanehirae than other six Lauracea species. Six validated insertion/deletions of bases in the DNA (InDels) are suitable for differentiation of the two species. Moreover, high amounts of linalool and sesamin were present in the leaves of C. kanehirae, but not in C. micranthum. Overall, this study provided new insight for distinguishing the two species on the basis of the chemical composition of the leaves and the InDel markers.

UPLC-Q-TOF-MS/MS fingerprinting for rapid identification of the chemical constituents of Ermiao Wan

2015 ◽  
Vol 7 (3) ◽  
pp. 846-862 ◽  
Author(s):  
Guangli Yan ◽  
Di Zou ◽  
Aihua Zhang ◽  
Yunlong Tan ◽  
Hui Sun ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Rapid Identification ◽  
Determination Method ◽  
Tof Ms

In this work, a novel and efficient determination method for the rapid identification of multi-class chemical constituents of Ermiao Wan has been developed.

Sign in / Sign up

Export Citation Format

Share Document