A fluorescent aptasensor for sensitive detection of human hepatocellular carcinoma SMMC-7721 cells based on graphene oxide

2014 ◽  
Vol 6 (17) ◽  
pp. 6809-6814 ◽  
Author(s):  
Qin Xie ◽  
Yuyu Tan ◽  
Qiuping Guo ◽  
Kemin Wang ◽  
Baoyin Yuan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Graphene Oxide ◽  
Cancer Cells ◽  
Direct Detection ◽  
Fluorescence Method ◽  
Human Hepatocellular Carcinoma ◽  
Sensitive Detection ◽  
Fluorescent Aptasensor

We developed a simple, rapid and sensitive fluorescence method for the direct detection of cancer cells using a GO-based aptasensor.

scholarly journals Graphene Oxide Loaded with Protocatechuic Acid and Chlorogenic Acid Dual Drug Nanodelivery System for Human Hepatocellular Carcinoma Therapeutic Application

2021 ◽  
Vol 22 (11) ◽  
pp. 5786
Author(s):  
Kalaivani Buskaran ◽  
Mohd Zobir Hussein ◽  
Mohamad Aris Mohd Moklas ◽  
Mas Jaffri Masarudin ◽  
Sharida Fakurazi
Keyword(s):  
Hepatocellular Carcinoma ◽  
Graphene Oxide ◽  
Chlorogenic Acid ◽  
Hepg2 Cells ◽  
Protocatechuic Acid ◽  
Malignant Neoplasm ◽  
Human Hepatocellular Carcinoma ◽  
Cell Cytotoxicity ◽  
Anticancer Activities ◽  
Dual Drug

Hepatocellular carcinoma or hepatoma is a primary malignant neoplasm that responsible for 75–90% of all liver cancer in humans. Nanotechnology introduced the dual drug nanodelivery method as one of the initiatives in nanomedicine for cancer therapy. Graphene oxide (GO) loaded with protocatechuic acid (PCA) and chlorogenic acid (CA) have shown some anticancer activities in both passive and active targeting. The physicochemical characterizations for nanocomposites were conducted. Cell cytotoxicity assay and lactate dehydrogenase were conducted to estimate cell cytotoxicity and the severity of cell damage. Next, nanocomposite intracellular drug uptake was analyzed using a transmission electron microscope. The accumulation and localization of fluorescent-labelled nanocomposite in the human hepatocellular carcinoma (HepG2) cells were analyzed using a fluorescent microscope. Subsequently, Annexin V- fluorescein isothiocyanate (FITC)/propidium iodide analysis showed that nanocomposites induced late apoptosis in HepG2 cells. Cell cycle arrest was ascertained at the G2/M phase. There was the depolarization of mitochondrial membrane potential and an upregulation of reactive oxygen species when HepG2 cells were induced by nanocomposites. In conclusion, HepG2 cells treated with a graphene oxide–polyethylene glycol (GOP)–PCA/CA–FA dual drug nanocomposite exhibited significant anticancer activities with less toxicity compared to pristine protocatechuic acid, chlorogenic acid and GOP–PCA/CA nanocomposite, may be due to the utilization of a folic acid-targeting nanodrug delivery system.

scholarly journals Alginate/CaCO3 Hybrid Loaded with Sorafenib Tosylate and Gold Hexagons: A Model for Efficient Dual (Chemo-Radio) Treatment of HepG2 Cells

2021 ◽  
Author(s):  
Firas A. Sukkar ◽  
Medhat W. Shafaa ◽  
Mohamed S. El-Nagdy ◽  
Soheir S. Korraa ◽  
wael darwish
Keyword(s):  
Hepatocellular Carcinoma ◽  
Gold Nanoparticles ◽  
Cancer Cells ◽  
Hepg2 Cells ◽  
Human Hepatocellular Carcinoma ◽  
The Novel ◽  
Chemotherapeutic Drug ◽  
Hydrophobic Drug ◽  
Γ Radiation ◽  
Different Doses

Abstract In this work, we investigated the combinatorial cytotoxic actions of the chemotherapeutic drug (sorafenib tosylate, ST) in synergism with γ-radiation on human hepatocellular carcinoma (HepG2) cells. The novel radiosensitizer, hexagonal gold nanoparticles, was prepared and utilized To enhance the radiobiological response of HepG2 cells. A well-designed nanoporous alginate/CaCO3 hybrid was prepared as a biocompatible/biodegradable nanocarrier for co-delivery of the hydrophobic drug and the hydrophilic radiosensitizer to the cancer cells. Incubation of HepG2 cells with the nanoprobes followed by different doses (0, 3 or 6 Gy) of γ-radiation resulted in significant reduction of the cells viability as a result of the synergistic (chemo-radiation) cytotoxic actions.

scholarly journals De Novo Emergence of Mesenchymal Stem-Like CD105 + Cancer Cells by Cytotoxic Agents in Human Hepatocellular Carcinoma

2017 ◽  
Vol 10 (2) ◽  
pp. 184-189 ◽  
Author(s):  
Yoshimoto Nomura ◽  
Taro Yamashita ◽  
Naoki Oishi ◽  
Kouki Nio ◽  
Takehiro Hayashi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cancer Cells ◽  
De Novo ◽  
Human Hepatocellular Carcinoma ◽  
Cytotoxic Agents

Estrogen signalling through amphiregulin may be implicated in human hepatocellular carcinoma

2011 ◽  
Vol 5 (3) ◽  
pp. 153-160 ◽  
Author(s):  
Giuseppe Carruba ◽  
Vitale Miceli ◽  
Letizia Cocciadiferro ◽  
Maurizio Zarcone ◽  
Biagio Agostara ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Human Hepatocellular Carcinoma ◽  
Mrna Levels ◽  
Wild Type ◽  
Liver Cancer Cells ◽  
Huh7 Cells ◽  
Liver Tissues

AbstractBackground:We investigated aromatase (Aro)-driven estrogen formation in non-tumoral and malignant liver tissues and cells, also in relation to expression of the estrogen receptors α and β (ERα and ERβ) and amphiregulin (AREG), aiming to gain insights into the potential role of estrogens in human hepatocellular carcinoma (HCC).Materials and methods:Chromatographic and reverse transcriptase polymerase chain reaction (RT-PCR) analyses were used to assess activity and expression of the Aro enzyme and AREG as well as the expression of wild-type and variant ERs, both in vivo and in vitro.Results:Following 24 h and 72 h incubation of liver tissues or cells with testosterone, human HCC tissues and HepG2 hepatoma cells showed elevated Aro activity (estrogen formation, respectively, of 20% and 52%–99%). By contrast, no Aro activity could be detected in non-tumoral tissues and HA22T liver cancer cells. Cirrhotic samples and Huh7 cells exhibited intermediate enzyme activity, with estrogen formation of 4% and 34%, respectively. Markedly lower or undetectable Aro mRNA levels were observed in HA22T cells and non-tumoral liver tissues compared with HepG2 cells and HCC samples. Cirrhotic specimens displayed variable transcript levels. Interestingly, no or low expression of wild-type ERα and ERβ could be observed in liver cancer cells and malignant tissues. However, ubiquitous expression of the hERα46 variant and occasional expression of the hERβ2/Cx variant were observed in cancer tissues and cells.Conclusions:It is noteworthy that the pattern of wild-type ERα was inversely related to Aro, whilst AREG expression was consistently associated with that of Aro. This combined evidence suggests that locally elevated Aro activity may increase malignant cell proliferation also through AREG signalling.

scholarly journals The effects of hyperthermia on human hepatocellular carcinoma stem and mature cancer cells

2020 ◽  
Vol 19 (3) ◽  
pp. 265-268
Author(s):  
David Sontag ◽  
David A. Miles ◽  
Julia Uhanova ◽  
Micah Grubert Van Iderstine ◽  
Jiaqi Yang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cancer Cells ◽  
Human Hepatocellular Carcinoma
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