A facile method for detection of alkaline phosphatase activity based on the turn-on fluorescence of resorufin

2014 ◽  
Vol 6 (15) ◽  
pp. 6105-6109 ◽  
Author(s):  
Fangyuan Wang ◽  
Yongxin Li ◽  
Wenying Li ◽  
Qingfeng Zhang ◽  
Jian Chen ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Turn On ◽  
Alp Activity ◽  
Fluorescence Turn On

In the present study, a facile fluorescence turn-on approach for the detection of alkaline phosphatase (ALP) activity has been developed.

Real-Time Fluorescence Turn-On Detection of Alkaline Phosphatase Activity with a Novel Perylene Probe

2012 ◽  
Vol 8 (1) ◽  
pp. 276-281 ◽  
Author(s):  
Jian Chen ◽  
Huping Jiao ◽  
Wenying Li ◽  
Dongli Liao ◽  
Huipeng Zhou ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Real Time ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Turn On ◽  
Fluorescence Turn On

A dual-mode fluorescence “turn-on” biosensor based on an aggregation-induced emission luminogen

2014 ◽  
Vol 2 (12) ◽  
pp. 1717-1723 ◽  
Author(s):  
Zhegang Song ◽  
Yuning Hong ◽  
Ryan T. K. Kwok ◽  
Jacky W. Y. Lam ◽  
Bin Liu ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Activity Assay ◽  
Dual Mode ◽  
Aggregation Induced Emission ◽  
Fluorescent Biosensor ◽  
Turn On ◽  
Fluorescence Turn On

A dual-mode fluorescent biosensor based on TPE-TEG-PA is developed for protamine quantification and alkaline phosphatase activity assay.

Alkaline phosphatase activity from human osteosarcoma cell line SaOS-2: an isoenzyme standard for quantifying skeletal alkaline phosphatase activity in serum.

1989 ◽  
Vol 35 (2) ◽  
pp. 223-229 ◽  
Author(s):  
J R Farley ◽  
E Kyeyune-Nyombi ◽  
N M Tarbaux ◽  
S L Hall ◽  
D D Strong
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Cell Lines ◽  
Alkaline Phosphatase Activity ◽  
Osteosarcoma Cell ◽  
Kinetic Assay ◽  
Human Osteosarcoma ◽  
Alp Activity ◽  
Human Osteosarcoma Cell ◽  
Skeletal Alkaline Phosphatase

Abstract Earlier we described a kinetic assay for quantifying skeletal alkaline phosphatase (ALP) isoenzyme activity in serum. The precision of the assay depends on including ALP standards for the skeletal, hepatic, intestinal, and placental isoenzymes. We wondered whether human osteosarcoma cells could provide an efficient alternative to human bone or Pagetic serum as a source of the skeletal ALP standard. ALP activities prepared from five human osteosarcoma cell lines were compared with a bone-derived ALP standard with respect to heat stability and sensitivity to chemical effectors. Two of the cell lines (SaOS-2 and TE-85) contained ALP activities that resembled the bone-derived standard. We selected SaOS-2 cells for additional evaluation (as a potential source of isoenzyme standard), because they contained 40-50 times more ALP activity than did the TE-85 cells. To include the SaOS-2 cell-derived ALP activity in the quantitative isoenzyme assay, we diluted the enzyme in a solution containing heat-inactivated (i.e., ALP-negative) human serum. Surprisingly, this dilution caused a 60-125% increase in maximum enzyme activity. In the quantitative assay of ALP isoenzyme in serum, the SaOS-2 derived ALP was indistinguishable from the serum skeletal ALP standard, with respect to the above criteria and assay variations. Evidently ALP from SaOS-2 cells is suited as a standard for measuring skeletal ALP activity in this assay.

A MnO2nanosheets–o-phenylenediamine oxidative system for the sensitive fluorescence determination of alkaline phosphatase activity

2018 ◽  
Vol 10 (44) ◽  
pp. 5341-5346 ◽  
Author(s):  
Xionghong Tan ◽  
Zheng Li ◽  
Yanlin Du ◽  
Aixian Zheng ◽  
Yongyi Zeng ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Alp Activity ◽  
Fluorescence Determination ◽  
Oxidative System

A MnO2nanosheets–o-phenylenediamine (OPDA) oxidative system was developed for detecting ALP activity selectively, sensitively and conveniently.

Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiation

2015 ◽  
Vol 51 (15) ◽  
pp. 3270-3272 ◽  
Author(s):  
Eun-Kyung Lim ◽  
Joo Oak Keem ◽  
Hui-suk Yun ◽  
Jinyoung Jung ◽  
Bong Hyun Chung
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Gold Nanoparticle ◽  
Alkaline Phosphatase Activity ◽  
Osteoblast Differentiation ◽  
Alp Activity

Gold nanoparticle-conjugated fluorescent hydroxyapatite (AuFHAp) was developed as a smart nanoprobe for measuring alkaline phosphatase (ALP) activity.

Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3871-3877 ◽  
Author(s):  
Shengda Qi ◽  
Huanhuan Zheng ◽  
Hongyan Qin ◽  
Honglin Zhai
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Gold Nanoclusters ◽  
Inner Filter Effect ◽  
Alp Activity ◽  
Filter Effect ◽  
Sensitive Method

In this work, a simple and sensitive method based on the inner filter effect (IFE) of p-nitrophenol (PNP) on the fluorescence of gold nanoclusters (AuNCs) has been developed for detecting alkaline phosphatase (ALP) activity.

scholarly journals A ratiometric fluorescence sensor based on enzymatically activatable micellization of TPE derivatives for quantitative detection of alkaline phosphatase activity in serum

RSC Advances ◽  
2020 ◽  
Vol 10 (45) ◽  
pp. 26888-26894 ◽  
Author(s):  
Jeongmoo Lee ◽  
Seoyun Kim ◽  
Tae Hoon Kim ◽  
Seoung Ho Lee
Keyword(s):  
Aqueous Solution ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Fluorescence Sensor ◽  
Fluorescence Assay ◽  
Quantitative Detection ◽  
Ratiometric Fluorescence ◽  
Alp Activity

A novel ratiometric fluorescence assay via enzymatically activatable micellization in aqueous solution was devised for quantitative detection of alkaline phosphatase (ALP) activity.

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