scholarly journals Solid-Phase Assay for Luteinizing Hormone in Mouse Plasma

1976 ◽  
Vol 29 (2) ◽  
pp. 105 ◽  
Author(s):  
AA Gidley-Baird ◽  
BM Bindon
Keyword(s):  
Luteinizing Hormone ◽  
Sensitivity And Specificity ◽  
Solid Phase ◽  
Cross Reaction ◽  
Assay System ◽  
Mouse Plasma ◽  
Pregnant Mice ◽  
Solid Phase Assay ◽  
Made In ◽  
Pituitary Homogenate

A solid-phase tube assay for measuring LH levels in mouse plasma is described. The assay utilizes an antiserum to ovine LH and ovine LH standards and it measures LH levels in 20 III of plasma with a sensitivity of less than 0�6 ng/m!. Various parameters affecting the sensitivity and specificity of the assay were investigated. Serial dilutions of plasma from pregnant mice, a pituitary homogenate from mice and plasma from hypophysectomized mice, injected subcutaneously with ovine LB, ran parallel with ovine LH standards in plasma from hypophysectomized mice and plasma with low LH levels from intact mice. Ovine TSH showed about 12 % cross reaction in the assay system, whilst rat FSH and prolactin and also ovine FSH, prolactin and GH showed practically no cross reaction. Measurements of plasma LH levels have been made in hypophysectomized mice after injection with different vehicles containing 10 or 50llg LH or 50llg FSH per animal. Daily measurements of LH levels throughout pregnancy in the mouse show a rise in LH level prior to implantation and a further rise around mid-pregnancy which drops off sharply to levels which remain fairly constant until parturition when there is another rise.

A SOLID-PHASE RADIOIMMUNOASSAY FOR OVINE FOLLICLE-STIMULATING HORMONE

1973 ◽  
Vol 58 (2) ◽  
pp. 239-249 ◽  
Author(s):  
N. F. CUNNINGHAM ◽  
C. NANCY HEBERT
Keyword(s):  
Luteinizing Hormone ◽  
Blood Plasma ◽  
Solid Phase ◽  
Follicle Stimulating Hormone ◽  
Thyroid Stimulating Hormone ◽  
Cross Reaction ◽  
Sheep Blood ◽  
Solid Phase Radioimmunoassay ◽  
Stimulating Hormone

SUMMARY A radioimmunoassay using antibody-coated polystyrene tubes is described, which is suitable for the estimation of follicle-stimulating hormone (FSH) levels in sheep blood plasma. Ovine FSH was labelled with 125I by an enzymic method employing lactoperoxidase and H2O2, and was stable for several months at −15 °C. Antisera to ovine FSH were raised in rabbits. Standard curves for unlabelled FSH obtained by this method with different preparations of labelled FSH showed reproducible slopes from day to day. Ovine luteinizing hormone (LH) and thyroid-stimulating hormone (TSH) showed about 17% cross-reaction in the assay, necessitating the application of a correction to the FSH values for samples of plasma containing more than 15 ng of LH or TSH/ml. The FSH content of plasma from wethers and anoestrous ewes ranged from 20 to 120 ng/ml.

IgM alloantibody detection by M-MPHA, a new solid-phase assay system

2000 ◽  
Vol 23 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Tamai Toyohiro ◽  
Ishii Yasuyuki ◽  
Mazda Toshio
Keyword(s):  
Solid Phase ◽  
Assay System ◽  
Solid Phase Assay

A new solid-phase assay system using magnetic force on blood group serology

1996 ◽  
Vol 6 (4) ◽  
pp. 351-359 ◽  
Author(s):  
J. Ohgama ◽  
R. Yabe ◽  
T. Tamai ◽  
M. Nakamura ◽  
T. Mazda
Keyword(s):  
Blood Group ◽  
Magnetic Force ◽  
Solid Phase ◽  
Assay System ◽  
Blood Group Serology ◽  
Solid Phase Assay

114-P: Removing interference for solid phase assay: DTT treatment vs. heat inactivation

2009 ◽  
Vol 70 ◽  
pp. S69
Author(s):  
Patricia Willey ◽  
D. Phelan ◽  
G. Morris ◽  
T. Mohanakumar
Keyword(s):  
Solid Phase ◽  
Heat Inactivation ◽  
Solid Phase Assay

Detection of antinuclear antibodies by indirect immunofluorescence and by solid phase assay

2011 ◽  
Vol 10 (12) ◽  
pp. 801-808 ◽  
Author(s):  
Katrijn Op De Beeck ◽  
Pieter Vermeersch ◽  
Patrick Verschueren ◽  
René Westhovens ◽  
Godelieve Mariën ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Antinuclear Antibodies ◽  
Solid Phase ◽  
Solid Phase Assay

LUTEINIZING HORMONE LEVELS IN PLASMA AND URINE IN WOMEN DURING NORMAL MENSTRUAL CYCLES AND IN WOMEN TAKING COMBINED CONTRACEPTIVES OR CHLORMADINONE ACETATE

1969 ◽  
Vol 43 (4) ◽  
pp. 617-624 ◽  
Author(s):  
A. HILARY ORR ◽  
MAX ELSTEIN
Keyword(s):  
Oral Contraceptive ◽  
Luteinizing Hormone ◽  
Young Women ◽  
Menstrual Cycles ◽  
Hormone Levels ◽  
Chlormadinone Acetate ◽  
Combined Oral Contraceptive ◽  
Low Dosage ◽  
Made In

SUMMARY Radioimmunoassay measurements of luteinizing hormone (LH) levels were made in urine and plasma throughout normal menstrual cycles and cycles when either a combined oral contraceptive or low dosage continuous chlormadinone acetate were being taken by healthy young women. The typical mid-cycle ovulatory increase of LH was suppressed in the cycles when the combined contraceptive preparations were taken but was present in the majority of cycles during the administration of continuous chlormadinone acetate.

scholarly journals EVALUATION OF ELEVEN IMMUNOCHROMATOGRAPHIC ASSAYS FOR SARS-CoV-2 DETECTION: INVESTIGATING DENGUE CROSS-REACTION

2020 ◽  
Author(s):  
Beatriz Araujo Oliveira ◽  
Lea Campos de Oliveira ◽  
Franciane Mendes de Oliveira ◽  
Geovana Maria Pereira ◽  
Regina Maia de Souza ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
High Sensitivity ◽  
Diagnostic Techniques ◽  
Cross Reactivity ◽  
Cross Reaction ◽  
List Type ◽  
Rt Pcr ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Good Agreement

AbstractBackgroundCOVID-19 disease (Coronavirus disease 2019) caused by SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) is widespread worldwide, affecting more than 11 million people globally (July 6th, 2020). Diagnostic techniques have been studied in order to contain the pandemic. Immunochromatographic (IC) assays are feasible and low cost alternative for monitoring the spread of COVID-19 in the population.MethodsHere we evaluate the sensitivity and specificity of eleven different immunochromatographic tests in 98 serum samples from confirmed cases of COVID-19 through RT-PCR and 100 negative serum samples from blood donors collected in February 2019. Considering the endemic situation of Dengue in Brazil, we also evaluated the cross-reactivity with Dengue using 20 serum samples from patients with confirmed diagnosis for Dengue collected in early 2019 through four different tests.ResultsOur results demonstrated agreement between immunochromatographic assays and RT-PCR, especially after 10 days since the onset of symptoms. The evaluation of IgG and IgM antibodies combined demonstrated a strong level of agreement (0.85) of IC assays and RT-PCR. It was observed cross-reactivity between Dengue and COVID-19 using four different IC assays for COVID-19 diagnosis. The specificity of IC assays to detected COVID-19 IgM antibodies using Dengue serum samples varied from 80% to 85%; the specificity of IgG detection was 100% and total antibody was 95%.ConclusionsWe found high sensitivity, specificity and good agreement of IC assays, especially after 10 days onset of symptoms. However, we detected cross-reactivity between Dengue and COVID-19 mainly with IgM antibodies demonstrating the need for better studies about diagnostic techniques for these diseases.HighlightsImmunochromatographic assays demonstrated high sensitivity and specificity and good agreement with the gold-standard RT-PCR;Increase in sensitivity and specificity of assays using samples collected after the 10th day of symptoms;Cross-reaction with Dengue serology in evaluation of IgM.

scholarly journals The combination of Elephantopus scaber and Sauropus androgynus promotes erythroid lineages and modulates follicle-stimulating hormone and luteinizing hormone levels in pregnant mice infected with Escherichia coli

2021 ◽  
pp. 1398-1404
Author(s):  
Muhammad Sasmito Djati ◽  
Yuyun Ika Christina ◽  
Muhaimin Rifa'i
Keyword(s):  
Escherichia Coli ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Relative Number ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hormone Levels ◽  
E Coli ◽  
Pregnant Mice ◽  
Elephantopus Scaber ◽  
Stimulating Hormone

Background and Aim: Escherichia coli infection produces an adverse effect on the erythrocyte lineage and hormone levels during pregnancy. This study aimed to evaluate the effects of Elephantopus scaber (ES) and Sauropus androgynus (SA) in combination on circulating follicle-stimulating hormone (FSH) and luteinizing hormone (LH) levels and erythropoiesis changes in E. coli-infected pregnant mice. Materials and Methods: Female Balb/c mice were mated with normal male mice and pregnancies were identified by the formation of vaginal plugs. Twenty-eight pregnant mice were divided randomly into seven groups: A control group (N), E. coli-infected pregnant mice (K+), and infected pregnant mice received the following five treatments: (1) Only ES; (2) ESSA1 (75:25); (3) ESSA2 (50:50); (4) ESSA3 (25:75); and (5) only SA, beginning from the 1st to the 16th day of pregnancy. Pregnant mice were infected with 107 CFU/mL of E. coli on day 4. Blood serum was collected on days 8, 12, and 16 of pregnancy and LH and FSH levels were measured by enzyme-linked immunosorbent assay. Bone marrow was isolated to determine the relative number of TER-119+VLA4+ and TER-119+CD34+ using flow cytometry. Results: The ESSA1 and SA groups exhibited a marked increase in LH levels. The combination of ES and SA administered at a 25:75 ratio (ESSA3) altered FSH levels and the relative number of TER-119+VLA4+ in infected pregnant mice. Combined with SA at an equal ratio (50:50), ESSA2 group exhibited a significant increase in the expression of TER119+CD34+ compared with the other treatment groups. Conclusion: ES and SA combined at a ratio of 25:75 exhibited optimal results in altering hormonal and erythropoiesis in infected pregnant mice.

The use of heterologous radioimmunoassays for the measurement of follicle-stimulating hormone and luteinizing hormone concentrations in horse and donkey serum

1983 ◽  
Vol 99 (2) ◽  
pp. 199-209 ◽  
Author(s):  
Valerie Urwin
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Cross Reactivity ◽  
Chorionic Gonadotrophin ◽  
Cross Reaction ◽  
The Other ◽  
Corpora Lutea ◽  
Serum Concentrations ◽  
Study Support ◽  
Baseline Levels

Heterologous double-antibody radioimmunoassays were developed for the measurement of FSH and LH concentrations in the serum of both horses and donkeys. The FSH assay employed a rabbit anti-ovine FSH serum which showed a complete lack of cross-reaction with equine chorionic gonadotrophin (eCG) and negligible cross-reaction with equine LH. The LH assay utilized an antiserum raised against highly purified eCG. This similarly showed negligible cross-reaction with equine FSH but its high cross-reactivity with eCG prevented the measurement of equine LH concentrations in serum when eCG was also present. In both assays serial dilutions of horse and donkey serum were parallel to the standard. The assays were used to monitor changes in serum concentrations of FSH and LH during the first 100 days of pregnancy in pony mares and jenny donkeys. In both species during pregnancy LH levels reached a peak 1–2 days after ovulation. They then decreased rapidly to baseline levels where they remained until days 35–40 when the commencement of eCG production prevented their further measurement. Serum FSH concentrations, on the other hand, continued to fluctuate markedly throughout the first 100 days of pregnancy in both the ponies and donkeys. Pronounced surges in FSH levels occurred at regular intervals in some animals but the pattern of release was quite irregular in the others. The results of this study support the concept that it is continued pituitary FSH release, not eCG secretion, which is responsible for stimulating the secondary follicles which develop during early equine pregnancy. However, it appears likely that it is the LH-like activity of eCG which causes the subsequent ovulation and/or luteinization of these secondary follicles to produce accessory corpora lutea.

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