Aqueous-filled polymer microcavity arrays: versatile & stable lipid bilayer platforms offering high lateral mobility to incorporated membrane proteins

The Analyst ◽  
2015 ◽  
Vol 140 (9) ◽  
pp. 3012-3018 ◽  
Author(s):  
Hajra Basit ◽  
Vinnie Gaul ◽  
Sean Maher ◽  
Robert J. Forster ◽  
Tia E. Keyes
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
Membrane Model ◽  
Lateral Mobility ◽  
Spherical Cap ◽  
Filled Polymer ◽  
Cell Membrane Model

A robust new supported cell membrane model is described comprising lipid bilayers supported on aqueous filled spherical cap pores in PDMS, both lipid and reconstituted membrane proteins diffuse unhindered by the underlying support.

scholarly journals The lipid bilayer membrane and its protein constituents

2018 ◽  
Vol 150 (11) ◽  
pp. 1472-1483 ◽  
Author(s):  
Janice L. Robertson
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Lipid Bilayer ◽  
Lipid Bilayers ◽  
External Solution ◽  
Bilayer Membrane ◽  
Lipid Bilayer Membrane ◽  
General Physiology ◽  
Selective Permeability ◽  
Solvent Environment

In 1918, the year the Journal of General Physiology was founded, there was little understanding of the structure of the cell membrane. It was evident that cells had invisible barriers separating the cytoplasm from the external solution. However, it would take decades before lipid bilayers were identified as the essential constituent of membranes. It would take even longer before it was accepted that there existed hydrophobic proteins that were embedded within the membrane and that these proteins were responsible for selective permeability in cells. With a combination of intuitive experiments and quantitative thinking, the last century of cell membrane research has led us to a molecular understanding of the structure of the membrane, as well as many of the proteins embedded within. Now, research is turning toward a physical understanding of the reactions of membrane proteins and lipids in this unique and incredibly complex solvent environment.

scholarly journals Antituberculosis Drug Interactions with Membranes: A Biophysical Approach Applied to Bedaquiline

Membranes ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 141
Author(s):  
Marina Pinheiro ◽  
Heinz Amenitsch ◽  
Salette Reis
Keyword(s):  
Cell Membrane ◽  
Eukaryotic Cell ◽  
Membrane Model ◽  
Molar Ratio ◽  
X Ray ◽  
X Ray Scattering ◽  
Membrane Models ◽  
Bacterial Cell Membrane ◽  
Cell Membrane Model ◽  
Ray Scattering

This work focuses on the interaction of the novel and representative antituberculosis (anti-TB) drug bedaquiline (BDQ) with different membrane models of eukaryotic and prokaryotic cells. The effect of BDQ on eukaryotic cell membrane models was assessed using liposomes, namely, multilamellar vesicles (MLVs) made of 1,2-dimyristoyl-rac-glycero-3-phosphocholine (DMPC) and also a mixture of DMPC and cholesterol (CHOL) (8:2 molar ratio). To mimic the prokaryotic cell membrane, 1,2-dimyristoyl-sn-glycero-3-phospho-rac-(1-glycerol) (DMPG) and 1,1′2,2′-tetra-oleoyl-cardiolipin (TOCL) were chosen. Powerful biophysical techniques were employed, including small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS), to understand the effect of BDQ on the nanostructure of the membrane models. The results showed that BDQ demonstrated a pronounced disordering effect in the bacterial cell membrane models, especially in the membrane model with cardiolipin (CL), while the human cell membrane model with large fractions of neutral phospholipids remained less affected. The membrane models and techniques provide detailed information about different aspects of the drug–membrane interaction, thus offering valuable information to better understand the effect of BDQ on their target membrane-associated enzyme as well as its side effects on the cardiovascular system.

INTERACTION OF LOCAL ANESTHETICS AND CELL MEMBRANE MODEL

1979 ◽  
Vol 51 (3) ◽  
pp. S231-S231
Author(s):  
H. Goto ◽  
D. Shieh ◽  
K. Arakawa
Keyword(s):  
Cell Membrane ◽  
Local Anesthetics ◽  
Membrane Model ◽  
Cell Membrane Model

scholarly journals A coarse-grained red blood cell membrane model to study stomatocyte-discocyte-echinocyte morphologies

PLoS ONE ◽  
2019 ◽  
Vol 14 (4) ◽  
pp. e0215447 ◽  
Author(s):  
Nadeeshani Maheshika Geekiyanage ◽  
Marie Anne Balanant ◽  
Emilie Sauret ◽  
Suvash Saha ◽  
Robert Flower ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Membrane Model ◽  
Coarse Grained ◽  
Red Blood Cell Membrane ◽  
Cell Membrane Model
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