A sensitive one-step method for quantitative detection of α-amylase in serum and urine using a personal glucose meter

The Analyst ◽  
2015 ◽  
Vol 140 (4) ◽  
pp. 1161-1165 ◽  
Author(s):  
Qing Wang ◽  
Hui Wang ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Rongjuan Liu ◽  
...  
Keyword(s):  
Amylase Activity ◽  
Direct Determination ◽  
Quantitative Detection ◽  
Step Method ◽  
Personal Glucose Meter ◽  
One Step ◽  
Glucose Meter ◽  
Serum And Urine

A one-step assay for direct determination of α-amylase activity in serum and urine was developed using a portable personal glucose meter.

One-step detection of hexokinase activity using a personal glucose meter

2018 ◽  
Vol 10 (18) ◽  
pp. 2075-2080 ◽  
Author(s):  
Jie Bai ◽  
Liyan Liu ◽  
Yanmei Han ◽  
Congcong Jia ◽  
Cuixia Liang
Keyword(s):  
Hexokinase Activity ◽  
Step Detection ◽  
Personal Glucose Meter ◽  
One Step ◽  
Glucose Meter

Using commercially available personal glucose meters for the determination of hexokinase activity.

scholarly journals A one-step method for quantitative determination of uracil in DNA by real-time PCR

2010 ◽  
Vol 38 (21) ◽  
pp. e196-e196 ◽  
Author(s):  
András Horváth ◽  
Beáta G. Vértessy
Keyword(s):  
Quantitative Determination ◽  
Real Time ◽  
Real Time Pcr ◽  
Step Method ◽  
One Step

One-step method for plasma determination of ibuprofen by chemiluminescence-coupled ultrafiltration and application in a pharmacokinetic study

Luminescence ◽  
2011 ◽  
Vol 27 (5) ◽  
pp. 371-378 ◽  
Author(s):  
Xunyu Xiong ◽  
Qunzheng Zhang ◽  
Yefei Nan ◽  
Xuefan Gu
Keyword(s):  
Pharmacokinetic Study ◽  
Step Method ◽  
One Step

Isopropylidine maltoheptosyl fructofuranoside, doubly blocked substrate for determination of endoamylase activity

1991 ◽  
Vol 37 (8) ◽  
pp. 1323-1328
Author(s):  
Z Ogawa ◽  
Y Matsubayashi ◽  
S Satoh ◽  
N Orita ◽  
H Itoh
Keyword(s):  
Human Serum ◽  
Coefficient Of Variation ◽  
Oxidation Rate ◽  
Amylase Activity ◽  
Mannitol Dehydrogenase ◽  
Coupled Reactions ◽  
Alpha Glucosidase ◽  
Serum And Urine

Abstract We synthesized o-(4,6-o-isopropylidene-alpha-D-glucopyranosyl)-(1----4)- [o-alpha-D-glucopyranosyl-(1----4])5-o-alpha-D-glucopyranosyl-(1----2)- alpha-D-fructofuranoside (IPG7F) and developed an assay for determining the activity of amylase in human serum and urine by using this substrate. Glucoamylase, alpha-glucosidase, and mannitol dehydrogenase are used as coupling enzymes. The coupled reactions are monitored by continuously measuring the oxidation rate of NADH. In this procedure, various substances in the test specimens do not interfere with the detection of amylase activity. Exactly one molecule of NADH is oxidized by one attack of amylase on the substrate, although four products can be produced in the reaction. The within-assay coefficient of variation (CV) ranged from 1.0% to 4.1% and the between-assay CV ranged from 2.6% to 5.3%. The results of our new assay correlate well with those of the amylase assay involving p-nitrophenol maltoheptaoside as substrate (r = 0.978) and with those of the amylase assay involving maltopentaose (r = 0.987).

A one-step method for the isolation and determination of leaf ribulose-1,5-diphosphate carboxylase

1971 ◽  
Vol 41 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Jonathan J. Goldthwaite ◽  
Lawrence Bogorad
Keyword(s):  
Step Method ◽  
One Step

Maltotetraose as a substrate for enzyme-coupled assay of amylase activity in serum and urine.

1979 ◽  
Vol 25 (3) ◽  
pp. 481-483 ◽  
Author(s):  
K J Whitlow ◽  
N Gochman ◽  
R L Forrester ◽  
L J Wataji
Keyword(s):  
Amylase Activity ◽  
Biological Fluids ◽  
Urine Samples ◽  
Coupled Assay ◽  
Serum And Urine ◽  
Serum And Urine Samples

Abstract The use of maltotetraose ss a new substrate for the enzyme-coupled determination of amylase activity in biological fluids was developed by Beckman Microbics. We evaluated a manual and a centrifugal analyzer version of the method in comparison with two commonly used manual starch-dye amylase techniques: Roche Amylochrome and Pharmacia Phadebas. Both maltotetraose amylase procedures proved to be rapid and precise, and results correlated satisfactorily with the starch-dye methods for serum and urine samples.

Digital quantitative detection of serum circulating miRNAs using dual-enhanced magnetobiosensors based on cascaded nucleic acid circuits

2019 ◽  
Vol 55 (91) ◽  
pp. 13733-13736 ◽  
Author(s):  
Chunxue Liu ◽  
Yayun An ◽  
Yuanfu Zhang ◽  
Xia Li ◽  
Qingwang Xue ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Quantitative Detection ◽  
Circulating Mirnas ◽  
Personal Glucose Meter ◽  
Glucose Meter

Here, we developed a dual-enhanced magnetobiosensor based on cascaded nucleic acid circuits for sensitive, portable and digital quantitative detection of circulating miRNAs in serum by a personal glucose meter (PGM).

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