Label-free phenotyping of peripheral blood lymphocytes by infrared imaging

The Analyst ◽  
2015 ◽  
Vol 140 (7) ◽  
pp. 2247-2256 ◽  
Author(s):  
M. Verdonck ◽  
S. Garaud ◽  
H. Duvillier ◽  
K. Willard-Gallo ◽  
E. Goormaghtigh
Keyword(s):  
Peripheral Blood ◽  
Infrared Imaging ◽  
Peripheral Blood Lymphocytes ◽  
Lymphocyte Subpopulations ◽  
Label Free ◽  
Ftir Imaging ◽  
Blood Lymphocytes ◽  
Antibody Labelling

FTIR imaging enables to effectively discriminate lymphocyte subpopulations without antibody labelling.

Lymphocyte subpopulations and responsiveness in rats bearing intracranial tumors induced by avian sarcoma virus

1981 ◽  
Vol 55 (4) ◽  
pp. 554-559 ◽  
Author(s):  
Thomas L. Roszman ◽  
William H. Brooks ◽  
William R. Markesbery ◽  
Gary J. Aziz ◽  
Darell D. Bigner
Keyword(s):  
Concanavalin A ◽  
Peripheral Blood ◽  
Absolute Number ◽  
Peripheral Blood Lymphocytes ◽  
Lymphocyte Subpopulations ◽  
Avian Sarcoma Virus ◽  
Content Type ◽  
Blood Lymphocytes ◽  
Sarcoma Virus ◽  
Avian Sarcoma

✓ The mitogenic responsiveness of peripheral blood lymphocytes obtained from Fischer 344 rats inoculated with avian sarcoma virus was studied. In addition, quantitative alterations in lymphocyte subpopulations were determined in these animals. Only peripheral blood lymphocytes from rats bearing astrocytomas had significantly diminished responses to concanavalin A when compared to control responses. The percentage of lymphocyte subpopulations detected in either the peripheral blood or spleen of tumor-bearing rats did not differ from values obtained with control rats. However, rats bearing astrocytomas had a marked decrease in the absolute number of the various lymphocyte subpopulations as a result of lymphopenia. Neither the sarcoma-bearing rats nor the virus-inoculated rats that did not develop tumors exhibited this lymphopenia. In addition, sera from rats bearing astrocytomas diminished the concanavalin A reactivity of spleen cells obtained from normal rats. The results of this study establish the avian sarcoma virus-induced rat astrocytoma model as a useful immunological parallel for the human disease.

scholarly journals Phenotypes of Peripheral Blood Lymphocytes and Cytokine Expression in Polymyositis and Dermatomyositis before Treatment and after Clinical Remission

2012 ◽  
Vol 5 ◽  
pp. CMAMD.S10272 ◽  
Author(s):  
Yasuhiro Shimojima ◽  
Wataru Ishii ◽  
Masayuki Matsuda ◽  
Shu-ichi Ikeda
Keyword(s):  
Peripheral Blood ◽  
Clinical Remission ◽  
Peripheral Blood Lymphocytes ◽  
Cytokine Expression ◽  
Lymphocyte Subpopulations ◽  
Clinical Severity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Lymphocytes ◽  
Serum Cytokines ◽  
Th1 And Th2

Objective To investigate peripheral blood lymphocyte subpopulations, particularly helper T (Th) cells and cytokine expression before and after treatment in polymyositis (PM) and dermatomyositis (DM). Patients and Methods Ten patients with PM and 15 with DM were enrolled in this study. As a control we used 11 age-matched healthy subjects. We analyzed peripheral blood lymphocytes using flow cytometry. Serum cytokines were determined by enzyme-linked immunosorbent assay. Results Th2/Th1 and Th2/Th17 ratios were significantly higher in both PM and DM than in controls and this Th2-predominancy is marked in DM associated with interstitial pneumonia. IL-1β, IL-6 and TGF-β were significantly higher in both PM and DM and IL-4 showed a significant increase in DM when compared to controls. There was no significant correlation between either any lymphocyte subpopulations or serum cytokines and clinical severity markers such as the manual muscle strength test, serum creatine kinase, and the total CT score. Th2/Th17 in both PM and DM, as well as Th2/Th1 in the latter, significantly decreased after clinical remission compared with before treatment. Conclusions Th2-predominancy as shown by the increase in Th2/Th1 and Th2/Th17 ratios may suggest active disease in PM/DM but does not reflect clinical severity.

Quantification of T-lymphocyte subpopulations in brain injury

1986 ◽  
Vol 67 (1) ◽  
pp. 40-40
Author(s):  
T. B. Tolpegina ◽  
L. Yu. Zaitseva
Keyword(s):  
Brain Injury ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Fatal Outcome ◽  
Peripheral Blood Lymphocytes ◽  
Lymphocyte Subpopulations ◽  
Brain Contusion ◽  
Blood Lymphocytes ◽  
T Lymphocyte Subpopulations ◽  
Craniocerebral Injuries

The aim of the study was to assess the population and subpopulation composition of peripheral blood lymphocytes in patients with craniocerebral injuries. Twenty-six patients were under observation, of whom 10 were diagnosed with moderate brain contusion, and 16 with severe brain contusion (in 4 patients the trauma was complicated by the development of pneumonia with fatal outcome).

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