Rituximab–Au nanoprobes for simultaneous dark-field imaging and DAB staining of CD20 over-expressed on Raji cells

The Analyst ◽  
2014 ◽  
Vol 139 (22) ◽  
pp. 5660-5663 ◽  
Author(s):  
Lin Fan ◽  
Doudou Lou ◽  
Yu Zhang ◽  
Ning Gu
Keyword(s):  
Gold Nanoparticles ◽  
Malignant Lymphoma ◽  
Dark Field ◽  
Lymphoma Cells ◽  
Raji Cells ◽  
High Affinity ◽  
Dark Field Imaging ◽  
Field Imaging

A novel dual-modal cell immunodetection method based on both dark-field imaging and catalysis functions of gold nanoparticles has been established, where the Rituximab–Au conjugates were used as nanoprobes to label and image specifically the CD20 overexpressed on the surface of malignant lymphoma cells of Raji with high affinity.

Dark field imaging of rattle-type silica nanorattles coated gold nanoparticles <italic>in vitro</italic> and<italic> in vivo</italic>

2013 ◽  
Vol 58 (7) ◽  
pp. 531-536 ◽  
Author(s):  
LongFei TAN ◽  
FangQiong TANG ◽  
ChangHui FU ◽  
HuiYu LIU ◽  
Dong CHEN ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dark Field ◽  
Dark Field Imaging ◽  
Field Imaging

Objective-type dark-field imaging spectroscopy applied to monitor the orientation of nonspherical gold nanoparticles

2013 ◽  
Vol 52 (2) ◽  
pp. 023602 ◽  
Author(s):  
Yu Huang ◽  
Wei Zhang ◽  
Ji-Ming He ◽  
Zhao-Ming Chen ◽  
Dong-Yun Tang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Imaging Spectroscopy ◽  
Dark Field ◽  
Dark Field Imaging ◽  
Field Imaging ◽  
Objective Type

Unravelling the nature of the spongy dark material in aged Turkevich gold nanoparticles colloidal solutions by CytoViva® dark-field imaging and HRTEM analysis

Micron ◽  
2019 ◽  
Vol 121 ◽  
pp. 21-25 ◽  
Author(s):  
Fernando Menegatti de Melo ◽  
Mayara Klimuk Uchiyama ◽  
Marcelo Nakamura ◽  
Rodrigo Ken Kawassaki ◽  
Carlos Pelleschi Taborda ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dark Field ◽  
Colloidal Solutions ◽  
Dark Field Imaging ◽  
Dark Material ◽  
Field Imaging

Detection of a point defect in a silicon single crystal by high-resolution TEM

1995 ◽  
Vol 53 ◽  
pp. 466-467
Author(s):  
M. Awaji
Keyword(s):  
High Resolution ◽  
Single Crystal ◽  
Point Defect ◽  
Point Defects ◽  
Noise Level ◽  
Dark Field ◽  
Silicon Single Crystal ◽  
High Resolution Image ◽  
Dark Field Imaging ◽  
Field Imaging

It is necessary to improve the resolution, brightness and signal-to-noise ratio(s/n) for the detection and identification of point defects in crystals. In order to observe point defects, multi-beam dark-field imaging is one of the useful methods. Though this method can improve resolution and brightness compared with dark-field imaging by diffuse scattering, the problem of s/n still exists. In order to improve the exposure time due to the low intensity of the dark-field image and the low resolution, we discuss in this paper the bright-field high-resolution image and the corresponding subtracted image with reference to a changing noise level, and examine the possibility for in-situ observation, identification and detection of the movement of a point defect produced in the early stage of damage process by high energy electron bombardment.The high-resolution image contrast of a silicon single crystal in the [10] orientation containing a triple divacancy cluster is calculated using the Cowley-Moodie dynamical theory and for a changing gaussian noise level. This divacancy model was deduced from experimental results obtained by electron spin resonance. The calculation condition was for the lMeV Berkeley ARM operated at 800KeV.

scholarly journals Noninvasive, in vivo assessment of the cervical microcirculation using incident dark field imaging

2021 ◽  
Vol 135 ◽  
pp. 104145
Author(s):  
Yani P. Latul ◽  
Arnoud W. Kastelein ◽  
Patricia W.T. Beemster ◽  
Nienke E. van Trommel ◽  
Can Ince ◽  
...  
Keyword(s):  
Dark Field ◽  
Dark Field Imaging ◽  
Incident Dark Field Imaging ◽  
Field Imaging ◽  
In Vivo Assessment

scholarly journals Correlation of image quality parameters with tube voltage in X-ray dark-field chest radiography: a phantom study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andreas P. Sauter ◽  
Jana Andrejewski ◽  
Manuela Frank ◽  
Konstantin Willer ◽  
Julia Herzen ◽  
...  
Keyword(s):  
Image Quality ◽  
Imaging Modality ◽  
Signal Strength ◽  
Dark Field ◽  
Tube Voltage ◽  
X Ray ◽  
Dose Area Product ◽  
Dark Field Imaging ◽  
Field Imaging

AbstractGrating-based X-ray dark-field imaging is a novel imaging modality with enormous technical progress during the last years. It enables the detection of microstructure impairment as in the healthy lung a strong dark-field signal is present due to the high number of air-tissue interfaces. Using the experience from setups for animal imaging, first studies with a human cadaver could be performed recently. Subsequently, the first dark-field scanner for in-vivo chest imaging of humans was developed. In the current study, the optimal tube voltage for dark-field radiography of the thorax in this setup was examined using an anthropomorphic chest phantom. Tube voltages of 50–125 kVp were used while maintaining a constant dose-area-product. The resulting dark-field and attenuation radiographs were evaluated in a reader study as well as objectively in terms of contrast-to-noise ratio and signal strength. We found that the optimum tube voltage for dark-field imaging is 70 kVp as here the most favorable combination of image quality, signal strength, and sharpness is present. At this voltage, a high image quality was perceived in the reader study also for attenuation radiographs, which should be sufficient for routine imaging. The results of this study are fundamental for upcoming patient studies with living humans.

Sign in / Sign up

Export Citation Format

Share Document