Dark field imaging of rattle-type silica nanorattles coated gold nanoparticles <italic>in vitro</italic> and<italic> in vivo</italic>

2013 ◽  
Vol 58 (7) ◽  
pp. 531-536 ◽  
Author(s):  
LongFei TAN ◽  
FangQiong TANG ◽  
ChangHui FU ◽  
HuiYu LIU ◽  
Dong CHEN ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Dark Field ◽  
Dark Field Imaging ◽  
Field Imaging

scholarly journals Noninvasive, in vivo assessment of the cervical microcirculation using incident dark field imaging

2021 ◽  
Vol 135 ◽  
pp. 104145
Author(s):  
Yani P. Latul ◽  
Arnoud W. Kastelein ◽  
Patricia W.T. Beemster ◽  
Nienke E. van Trommel ◽  
Can Ince ◽  
...  
Keyword(s):  
Dark Field ◽  
Dark Field Imaging ◽  
Incident Dark Field Imaging ◽  
Field Imaging ◽  
In Vivo Assessment

scholarly journals Correlation of image quality parameters with tube voltage in X-ray dark-field chest radiography: a phantom study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andreas P. Sauter ◽  
Jana Andrejewski ◽  
Manuela Frank ◽  
Konstantin Willer ◽  
Julia Herzen ◽  
...  
Keyword(s):  
Image Quality ◽  
Imaging Modality ◽  
Signal Strength ◽  
Dark Field ◽  
Tube Voltage ◽  
X Ray ◽  
Dose Area Product ◽  
Dark Field Imaging ◽  
Field Imaging

AbstractGrating-based X-ray dark-field imaging is a novel imaging modality with enormous technical progress during the last years. It enables the detection of microstructure impairment as in the healthy lung a strong dark-field signal is present due to the high number of air-tissue interfaces. Using the experience from setups for animal imaging, first studies with a human cadaver could be performed recently. Subsequently, the first dark-field scanner for in-vivo chest imaging of humans was developed. In the current study, the optimal tube voltage for dark-field radiography of the thorax in this setup was examined using an anthropomorphic chest phantom. Tube voltages of 50–125 kVp were used while maintaining a constant dose-area-product. The resulting dark-field and attenuation radiographs were evaluated in a reader study as well as objectively in terms of contrast-to-noise ratio and signal strength. We found that the optimum tube voltage for dark-field imaging is 70 kVp as here the most favorable combination of image quality, signal strength, and sharpness is present. At this voltage, a high image quality was perceived in the reader study also for attenuation radiographs, which should be sufficient for routine imaging. The results of this study are fundamental for upcoming patient studies with living humans.

scholarly journals Method combining BAC film and positive staining for the characterization of DNA intermediates by dark-field electron microscopy

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Yann Benureau ◽  
Eliana Moreira Tavares ◽  
Ali-Akbar Muhammad ◽  
Sonia Baconnais ◽  
Eric Le Cam ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Dark Field ◽  
In Vitro Assays ◽  
Positive Staining ◽  
Synthetic Dna ◽  
Dark Field Imaging ◽  
Alkyl Ammonium

Abstract DNA intermediate structures are formed in all major pathways of DNA metabolism. Transmission electron microscopy (TEM) is a tool of choice to study their choreography and has led to major advances in the understanding of these mechanisms, particularly those of homologous recombination (HR) and replication. In this article, we describe specific TEM procedures dedicated to the structural characterization of DNA intermediates formed during these processes. These particular DNA species contain single-stranded DNA regions and/or branched structures, which require controlling both the DNA molecules spreading and their staining for subsequent visualization using dark-field imaging mode. Combining BAC (benzyl dimethyl alkyl ammonium chloride) film hyperphase with positive staining and dark-field TEM allows characterizing synthetic DNA substrates, joint molecules formed during not only in vitro assays mimicking HR, but also in vivo DNA intermediates.

Giant Faceting of Vicinal Si(001) Induced by Au Adsorption

1998 ◽  
Vol 05 (06) ◽  
pp. 1167-1178 ◽  
Author(s):  
F.-J. Meyer Zu Heringdorf ◽  
D. Kähler ◽  
M. Horn-Von Hoegen ◽  
Th. Schmidt ◽  
E. Bauer ◽  
...  
Keyword(s):  
Dark Field ◽  
Growth Behavior ◽  
Nucleation Process ◽  
Vicinal Surface ◽  
Dark Field Imaging ◽  
Parallel Arrangement ◽  
Kinetics Of ◽  
Field Imaging ◽  
Critical Coverage

4° vicinal Si(001) shows perfectly ordered terraces with a width of 4 nm which are separated by double steps. Adsorption of Au at 800°C results in a dramatic change of the step morphology: the surface decomposes into areas which are perfectly flat with a (001) orientation and (119) facets, which compensate for the macroscopic miscut. Extremely straight superterraces with a length limited only by the size of the sample (here 4 mm) and a width ranging from 400 nm to 4 μm are formed by massive Si mass transport. The extreme aspect ratio of 1:10 000 of this submicron structure is attributed to a heterogeneous nucleation process. SPA-LEED reveals a new, Au-induced incommensurate 5×3.2 reconstruction above a critical coverage as the driving force for the formation of large elongated (001) terraces. LEEM shows the strongly anisotropic nucleation process in vivo. Dark field imaging and microspot LEED techniques have been used to determine the influence of the different 5×3.2 domain orientations on the growth behavior of the (001) superterraces. The majority of domain terraces grow with a speed of more than 10 μm per second. The width and area of the (001) terraces increase proportionally to the Au coverage. The steps of the vicinal surface are accumulated in irregular step bunches. With further increasing Au coverage the step bunches are transformed into well-defined facets with a (119) orientation, as determined by SPA-LEED. The kinetics of the faceting process have been studied with SPA-LEED, REM, STM, and light diffraction using a HeNe laser, because the typical size of the superterraces is of the order of the wavelength of visible light: the resulting structure is visible to the bare eye. The parallel arrangement of superterraces acts as an irregular optical phase grating: illumination with white light results in stripes of all possible diffraction colors.

Dynamic In Vivo Chest X-ray Dark-Field Imaging in Mice

2019 ◽  
Vol 38 (2) ◽  
pp. 649-656 ◽  
Author(s):  
R. Gradl ◽  
K. S. Morgan ◽  
M. Dierolf ◽  
C. Jud ◽  
L. Hehn ◽  
...  
Keyword(s):  
Dark Field ◽  
X Ray ◽  
Dark Field Imaging ◽  
Chest X Ray ◽  
Field Imaging

Objective-type dark-field imaging spectroscopy applied to monitor the orientation of nonspherical gold nanoparticles

2013 ◽  
Vol 52 (2) ◽  
pp. 023602 ◽  
Author(s):  
Yu Huang ◽  
Wei Zhang ◽  
Ji-Ming He ◽  
Zhao-Ming Chen ◽  
Dong-Yun Tang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Imaging Spectroscopy ◽  
Dark Field ◽  
Dark Field Imaging ◽  
Field Imaging ◽  
Objective Type
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