Signal amplification by a self-assembled biosensor system designed on the principle of dockerin–cohesin interactions in a cellulosome complex

The Analyst ◽  
2014 ◽  
Vol 139 (19) ◽  
pp. 4790-4793 ◽  
Author(s):  
Jeong Eun Hyeon ◽  
Dae Hee Kang ◽  
Sung Ok Han
Keyword(s):  
Protein Complex ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Biosensor System ◽  
Self Assembled

A self-assembled protein complex based on the principle of a cellulosome system is proposed for a biosensor with high sensitivity due to signal amplification.

scholarly journals An enzyme-free molecular catalytic device: dynamically self-assembled DNA dendrimers for in situ imaging of microRNAs in live cells

2019 ◽  
Vol 10 (6) ◽  
pp. 1651-1658 ◽  
Author(s):  
Shuzhen Yue ◽  
Xinyue Song ◽  
Weiling Song ◽  
Sai Bi
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Live Cells ◽  
Sensitivity And Selectivity ◽  
Catalytic Hairpin Assembly ◽  
Self Assembled ◽  
In Situ Imaging

Branched catalytic hairpin assembly is proposed for dynamic self-assembly of DNA dendrimers for signal amplification and in situ imaging of microRNAs in live cells with high sensitivity and selectivity.

An ultrasensitive colorimetric aptasensor for ATP based on peptide/Au nanocomposites and hemin–G-quadruplex DNAzyme

RSC Advances ◽  
2014 ◽  
Vol 4 (44) ◽  
pp. 23185-23190 ◽  
Author(s):  
Shipeng Li ◽  
Liqiang Wang ◽  
Yuanqiang Hao ◽  
Lili Zhang ◽  
Binbin Zhou ◽  
...  
Keyword(s):  
Signal Amplification ◽  
High Sensitivity ◽  
G Quadruplex ◽  
Self Assembled ◽  
Amplification Strategy ◽  
Colorimetric Aptasensor ◽  
Atp Detection

A self-assembled peptide nanosphere was firstly applied to construct biosensors. A new signal amplification strategy was proposed for colorimetric aptasensor based on PNS/AuNPs composite. The colorimetric aptasensor displayed an ultra-high sensitivity for ATP detection with a LOD of 1.35 pM.

scholarly journals Three-dimensional self-assembled hybrid silicon nanostructures for high sensitivity SERS chemical and biosensing applications

2021 ◽  
Author(s):  
Jeffery Alexander Powell
Keyword(s):  
Noble Metal ◽  
Three Dimensional ◽  
High Sensitivity ◽  
Metal Nanostructures ◽  
Silicon Nanostructures ◽  
Sers Enhancement ◽  
Noble Metal Nanostructures ◽  
Self Assembled ◽  
Hybrid Silicon ◽  
Si Nanostructures

Raman spectroscopy is a powerful tool for detection of chemical and bioanalytes but lacks enhancement required to detect these analytes at the ultrahigh sensitivity needed for many applications. Surface enhanced Raman Scattering is a technique by which an analyte signal can become greatly enhanced and, near single molecule sensitivity, is achievable. Currently, SERS-based detection platforms currently rely on noble metal nanostructures as primary enhancing sources for the detection of chemical and bioanalytes but have significant limitations in terms of reproducibility and biocompatibility. Recent research has shown that semiconductors have the ability to exhibit SERS enhancing characteristics that can potentially supplant the use of noble metals without the limitations associated with noble metal nanomaterials. This thesis presents, the generation of three-dimensional self-assembled hybrid silicon nanostructures though a laser-ion plume formation mechanism. These Si nanostructures exhibit high sensitivity SERS enhancement characteristics which can be applied for chemical and biosensing applications. In this thesis, the Raman enhancing characteristics of the hybrid Si nanostructures are examined and correlated to the unique physical morphology and material chemistry of these nanostructures. These Si nanostructures are shown to be comprised of individual Si nanospheroids that have fused to form a highly 3D nanoweb-like self-assembled nanostructures. It is also shown that these nanospheroids are composed of both amorphous and polycrystalline sub-regions, which can only be generated within an ion-plume formed by a femtosecond pulsed laser. By programming the laser, the nanostructure morphology and hybrid nature can be manipulated and optimized. These Si nanostructures are shown to be highly sensitive as SERS platforms for chemical analytes. In addition, it is shown that with the application of noble metal nanoparticles on the surface of the 3D hybrid silicon nanowebs structures, an additional enhancement boost can be optimized for the detection of chemical molecules. With this, the dual contribution to the SERS sensitivity from both the primary Si nanostructures and the secondary noble metal nanostructures can be used to detect the presence of a biomolecule analyte is shown. To delve deeper into how these hybrid Si nanostructures cause SERS enhancement of bioanalytes, the Si ion interactions within the laser-ion plume were manipulated to induce quantum-scale defects within the hybrid Si nanospheroids. By creating both an inert and oxygenated laser-ion plumes the formation of sub-nanograins within the nanospheroids and sub-nanovoids on the nanospheroid surface is shown to significantly enhance the detection of bioanalyte signal for multiple biomolecules which act as signals for various diseases. Based on the results in this thesis, it has been proven that Si-based nanostructures have the capacity to be used as sole SERS enhancing sources for chemical and biomolecule analytes.

A highly sensitive humidity sensor based on an aggregation-induced emission luminogen-appended hygroscopic polymer microresonator

2021 ◽  
Author(s):  
Airong Qiagedeer ◽  
Hiroshi Yamagishi ◽  
Minami Sakamoto ◽  
Hanako Hasebe ◽  
Fumitaka Ishiwari ◽  
...  
Keyword(s):  
Humidity Sensor ◽  
High Sensitivity ◽  
Aggregation Induced Emission ◽  
Highly Sensitive ◽  
Self Assembled

A self-assembled microsphere resonator, comprising a hygroscopic polymer with aggregation-induced emission luminogen pendants, can sense humidity with high sensitivity and repeatability.

T7 exo-mediated FRET-breaking combined with DSN–RNAse–TdT for the detection of microRNA with ultrahigh signal-amplification

The Analyst ◽  
2019 ◽  
Vol 144 (10) ◽  
pp. 3216-3220 ◽  
Author(s):  
Van Thang Nguyen ◽  
Binh Huy Le ◽  
Young Jun Seo
Keyword(s):  
False Positive ◽  
Signal Amplification ◽  
High Sensitivity ◽  
Positive Signal ◽  
False Positive Signal ◽  
Breaking Mechanism ◽  
Very High

A DSN–RNAse–TdT–T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity—the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo.

scholarly journals Development of a Novel Enhanced Biosensor System for Real-Time Monitoring of Fish Stress Using a Self-Assembled Monolayer

Sensors ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1518 ◽  
Author(s):  
Haiyun Wu ◽  
Yuzu Fujii ◽  
Toshiki Nakano ◽  
Takafumi Arimoto ◽  
Masataka Murata ◽  
...  
Keyword(s):  
Real Time ◽  
Stress Responses ◽  
Glucose Concentration ◽  
Sensor Response ◽  
Stress Monitoring ◽  
Output Current ◽  
Self Assembled Monolayer ◽  
Biosensor System ◽  
Self Assembled ◽  
Wireless Biosensor

Wireless biosensor systems were developed in our lab for monitoring blood glucose concentrations in fish as an indicator of fish stress. However, uniform immobilization of the enzyme on the surface of the electrode is difficult, so the sensor response is typically reduced at a range of high glucose concentrations during the stress monitoring. In this study, we attempted to enhance sensor response by using a self-assembled monolayer-immobilized enzyme. Glucose oxidase was immobilized on a working electrode modified with a self-assembled monolayer. The proposed biosensor showed a good correlation between the output current and the glucose concentration range of 10–3500 mg dL−1 under an optimized working condition. The dynamic measurement range of this newly developed sensor is significantly improved, especially over a high concentration range, which helps the sensor to achieve better performance in dramatic changes in the stress response of fish. In addition, we used biological samples from test fish and obtained a good correlation coefficient between the sensor output current and the glucose concentration using a conventional method. The proposed wireless biosensor system was also applied to monitor fish stress responses in real time through different stressors and to obtain some precise data that reflect real fish stress responses.

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