scholarly journals Single vs. two-photon microscopy for label free intrinsic tissue studies in the UV light region

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2663-2667 ◽  
Author(s):  
Vitalijs Zubkovs ◽  
Frédéric Jamme ◽  
Slavka Kascakova ◽  
Franck Chiappini ◽  
François Le Naour ◽  
...  
Keyword(s):  
Single Photon ◽  
Uv Light ◽  
Label Free ◽  
Tissue Diagnosis ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Ultraviolet Microscopy ◽  
Light Region

Single photon ultraviolet microscopy is complementary to two-photon microscopy for tissue diagnosis.

scholarly journals Cell and brain tissue imaging of the flavonoid fisetin using label-free two-photon microscopy

2015 ◽  
Vol 89 ◽  
pp. 243-248 ◽  
Author(s):  
Tatiana B. Krasieva ◽  
Jennifer Ehren ◽  
Thomas O'Sullivan ◽  
Bruce J. Tromberg ◽  
Pamela Maher
Keyword(s):  
Brain Tissue ◽  
Tissue Imaging ◽  
Label Free ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Two-photon fluorescence lifetime for label-free microfluidic droplet sorting

2021 ◽  
Author(s):  
Sadat Hasan ◽  
Maximilian E. Blaha ◽  
Sebastian K. Piendl ◽  
Anish Das ◽  
David Geissler ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Single Photon ◽  
Photon Counting ◽  
Droplet Microfluidics ◽  
Label Free ◽  
Single Photon Counting ◽  
Two Photon ◽  
Deep Uv ◽  
Droplet Sorting ◽  
532 Nm

AbstractMicrofluidic droplet sorting systems facilitate automated selective micromanipulation of compartmentalized micro- and nano-entities in a fluidic stream. Current state-of-the-art droplet sorting systems mainly rely on fluorescence detection in the visible range with the drawback that pre-labeling steps are required. This limits the application range significantly, and there is a high demand for alternative, label-free methods. Therefore, we introduce time-resolved two-photon excitation (TPE) fluorescence detection with excitation at 532 nm as a detection technique in droplet microfluidics. This enables label-free in-droplet detection of small aromatic compounds that only absorb in a deep-UV spectral region. Applying time-correlated single-photon counting, compounds with similar emission spectra can be distinguished due to their fluorescence lifetimes. This information is then used to trigger downstream dielectrophoretic droplet sorting. In this proof-of-concept study, we developed a polydimethylsiloxane-fused silica (FS) hybrid chip that simultaneously provides a very high optical transparency in the deep-UV range and suitable surface properties for droplet microfluidics. The herein developed system incorporating a 532-nm picosecond laser, time-correlated single-photon counting (TCSPC), and a chip-integrated dielectrophoretic pulsed actuator was exemplarily applied to sort droplets containing serotonin or propranolol. Furthermore, yeast cells were screened using the presented platform to show its applicability to study cells based on their protein autofluorescence via TPE fluorescence lifetime at 532 nm. Graphical abstract

Automated label‐free detection of injured neuron with deep learning by two‐photon microscopy

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Shu Wang ◽  
Bingbing Lin ◽  
Guimin Lin ◽  
Ruolan Lin ◽  
Feng Huang ◽  
...  
Keyword(s):  
Deep Learning ◽  
Label Free ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Label Free Detection

Label-free imaging of healthy and infarcted fetal sheep hearts by two-photon microscopy

2017 ◽  
Vol 11 (1) ◽  
pp. e201600296 ◽  
Author(s):  
Alexandra Sorvina ◽  
Christie A. Bader ◽  
Mitchell C. Lock ◽  
Douglas A. Brooks ◽  
Janna L. Morrison ◽  
...  
Keyword(s):  
Label Free ◽  
Fetal Sheep ◽  
Two Photon Microscopy ◽  
Two Photon

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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