scholarly journals Scope and limitation of label-free multiphoton microscopy and probe-labeled two-photon microscopy for the endomicroscopic diagnosis

Scanning ◽  
2013 ◽  
Vol 36 (4) ◽  
pp. 462-464 ◽  
Author(s):  
Hoon Jai Chun ◽  
Eun Sun Kim ◽  
Bong Rae Cho
Keyword(s):  
Multiphoton Microscopy ◽  
Label Free ◽  
Two Photon Microscopy ◽  
Two Photon

Intravital multiphoton microscopy of dynamic renal processes

2005 ◽  
Vol 288 (6) ◽  
pp. F1084-F1089 ◽  
Author(s):  
Bruce A. Molitoris ◽  
Ruben M. Sandoval
Keyword(s):  
Proximal Tubule ◽  
Multiphoton Microscopy ◽  
Field Of View ◽  
Glomerular Permeability ◽  
Structural Alterations ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Dynamic Events ◽  
Subcellular Resolution ◽  
Functioning Kidney

Recent advances in microscopy and optics, computer sciences, and the available fluorophores used to label molecules of interest have empowered investigators to utilize intravital two-photon microscopy to study the dynamic events within the functioning kidney. This emerging technique enables investigators to follow functional and structural alterations with subcellular resolution within the same field of view over seconds to weeks. This approach invigorates the validity of data and facilitates analysis and interpretation as trends are more readily determined when one is more closely monitoring indicative physiological parameters. Therefore, in this review we emphasize how specific approaches will enable studies into glomerular permeability, proximal tubule endocytosis, and microvascular function within the kidney. We attempt to show how visual data can be quantified, thus allowing enhanced understanding of the process under study. Finally, emphasis is given to the possible future opportunities of this technology and its present limitations.

scholarly journals Extended depth of focus multiphoton microscopy via incoherent pulse splitting

2020 ◽  
Author(s):  
Bingying Chen ◽  
Tonmoy Chakraborty ◽  
Stephan Daetwyler ◽  
James D. Manton ◽  
Kevin Dean ◽  
...  
Keyword(s):  
Multiphoton Microscopy ◽  
Laser Pulses ◽  
Low Complexity ◽  
Phase Mask ◽  
Light Sources ◽  
Depth Of Focus ◽  
Ultrafast Laser Pulses ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Extended Depth Of Focus

AbstractWe present a phase mask that can be easily added to any multi-photon raster scanning microscope to extend the depth of focus five-fold at a small loss in lateral resolution. The method is designed for ultrafast laser pulses or other light-sources featuring a low coherence length. In contrast to other methods of focus extension, our approach uniquely combines low complexity, high light-throughput and multicolor capability. We characterize the point-spread function in a two-photon microscope and demonstrate fluorescence imaging of GFP labeled neurons in fixed brain samples as imaged with conventional and extended depth of focus two-photon microscopy.

scholarly journals Cell and brain tissue imaging of the flavonoid fisetin using label-free two-photon microscopy

2015 ◽  
Vol 89 ◽  
pp. 243-248 ◽  
Author(s):  
Tatiana B. Krasieva ◽  
Jennifer Ehren ◽  
Thomas O'Sullivan ◽  
Bruce J. Tromberg ◽  
Pamela Maher
Keyword(s):  
Brain Tissue ◽  
Tissue Imaging ◽  
Label Free ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Single vs. two-photon microscopy for label free intrinsic tissue studies in the UV light region

The Analyst ◽  
2014 ◽  
Vol 139 (11) ◽  
pp. 2663-2667 ◽  
Author(s):  
Vitalijs Zubkovs ◽  
Frédéric Jamme ◽  
Slavka Kascakova ◽  
Franck Chiappini ◽  
François Le Naour ◽  
...  
Keyword(s):  
Single Photon ◽  
Uv Light ◽  
Label Free ◽  
Tissue Diagnosis ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Ultraviolet Microscopy ◽  
Light Region

Single photon ultraviolet microscopy is complementary to two-photon microscopy for tissue diagnosis.

Automated label‐free detection of injured neuron with deep learning by two‐photon microscopy

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Shu Wang ◽  
Bingbing Lin ◽  
Guimin Lin ◽  
Ruolan Lin ◽  
Feng Huang ◽  
...  
Keyword(s):  
Deep Learning ◽  
Label Free ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Label Free Detection

scholarly journals In Vivo Simultaneous Nonlinear Absorption Raman and Fluorescence (SNARF) Imaging of Mouse Brain Cortical Structures

2021 ◽  
Author(s):  
Andrew T Francis ◽  
Bryce Manifold ◽  
Elena C Thomas ◽  
Ruoqian Hu ◽  
Andrew H Hill ◽  
...  
Keyword(s):  
Nonlinear Absorption ◽  
Transient Absorption ◽  
Stimulated Raman Scattering ◽  
Multiphoton Microscopy ◽  
Label Free ◽  
Two Photon ◽  
Age Dependent ◽  
Vessel Structure ◽  
Microscopy Techniques

Two photon excited fluorescence (TPEF) microscopy is a widely used optical imaging technique that has revolutionized neurophotonics through a diverse palette of dyes, specialized transgenic models, easy implementation, and straightforward data analysis. However, in vivo TPEF imaging is often limited in the number of contrasts available to distinguish different cells, structures, or functions. We propose using two label free multiphoton microscopy techniques: stimulated Raman scattering (SRS) microscopy and transient absorption microscopy (TAM) as complementary and orthogonal imaging modalities to TPEF for in vivo brain imaging. In this study, we construct a simultaneous nonlinear absorption, Raman, and fluorescence (SNARF) microscope and image several cortical structures up to 250-300 μm below the pial surface, the highest reported in vivo imaging depth for SRS or TAM. We further demonstrate the capabilities of our SNARF microscope through the quantification of age-dependent myelination, hemodynamics, vessel structure, cell density, and cell identity in vivo. Using machine learning, we report the use of label free SRS and TAM features to predict capillary lining cell identities with 90% accuracy. The SNARF microscope and methodology outlined herein provide a powerful platform to study several research topics, including neurovascular coupling, blood brain barrier, neuronal and axonal degeneration in aging, and neurodegenerative diseases.

Two-Photon Microscopy as a Useful Tool for Elucidating the Mode of Action of Novel Potential Cancer Therapeutics.

1999 ◽  
Vol 5 (S2) ◽  
pp. 1064-1065
Author(s):  
C. S. Navara ◽  
F. M. Uckun
Keyword(s):  
Plasma Membrane ◽  
Multiphoton Microscopy ◽  
Three Dimensional ◽  
Cancer Therapeutics ◽  
Leukemic Cell ◽  
Time Resolved ◽  
Two Photon Microscopy ◽  
Cellular Effects ◽  
Two Photon ◽  
Potential Cancer

Multiphoton microscopy allows us to follow specific cellular structures (ie. nuclei, mitochondria, and the plasma membrane) in a high resolution three dimensional and time resolved fashion. We have used multiphoton confocal microscopy to evaluate the cellular effects of potential cancer therapeutics. Cancer cells were plated onto coverslips and labelled with either the vital DNA dye Hoechst to image the nuclei and chromosomes or the lipid dye DiA to label the plasma membrane. Both of these dyes can be imaged with several sections without affecting cell viability as assessed by cell motility and the ability to undergo mitosis and cytokinesis.We have used this technology to show that one compound (DDE 131) rapidly induces in cancer cell lines the hallmarks of apoptosis; DNA hypercondensation, nuclear fragmentation and rapid membrane blebbing. In a leukemic cell line these changes take place within 20 minutes of treatment.

Label-free imaging of healthy and infarcted fetal sheep hearts by two-photon microscopy

2017 ◽  
Vol 11 (1) ◽  
pp. e201600296 ◽  
Author(s):  
Alexandra Sorvina ◽  
Christie A. Bader ◽  
Mitchell C. Lock ◽  
Douglas A. Brooks ◽  
Janna L. Morrison ◽  
...  
Keyword(s):  
Label Free ◽  
Fetal Sheep ◽  
Two Photon Microscopy ◽  
Two Photon

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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