Co-immobilized poly(ethylene glycol)-block-polyamines promote sensitivity and restrict biofouling on gold sensor surface for detecting factor IX in human plasma

The Analyst ◽  
2014 ◽  
Vol 139 (16) ◽  
pp. 3977-3985 ◽  
Author(s):  
Thangavel Lakshmipriya ◽  
Yukichi Horiguchi ◽  
Yukio Nagasaki
Keyword(s):  
Surface Plasmon Resonance ◽  
Ethylene Glycol ◽  
Surface Plasmon ◽  
Coagulation Factor ◽  
Factor Ix ◽  
Sensor Surface ◽  
Poly Ethylene Glycol ◽  
Spr Sensor ◽  
Coagulation Factor Ix ◽  
Poly Ethylene

In order to detect a low amount of human coagulation factor IX (FIX), poly(ethylene glycol) (PEG)/aptamer co-immobilized surface was constructed using original PEG–polyamine on surface plasmon resonance (SPR) sensor chip.

Molecularly imprinted surface plasmon resonance (SPR) based sensing of bisphenol A for its selective detection in aqueous systems

2015 ◽  
Vol 7 (11) ◽  
pp. 4661-4670 ◽  
Author(s):  
Huma Shaikh ◽  
Gulsu Sener ◽  
Najma Memon ◽  
Muhammad Iqbal Bhanger ◽  
Shafi Muhammad Nizamani ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Ethylene Glycol ◽  
Bisphenol A ◽  
Film Deposition ◽  
Selective Detection ◽  
Glycol Dimethacrylate ◽  
Poly Ethylene Glycol ◽  
Molecularly Imprinted ◽  
Spr Sensor ◽  
Poly Ethylene

Bisphenol A (BPA) imprinted poly(ethylene glycol dimethacrylate-N-methacryloyl-l-phenylalanine-vinyl imidazole) [poly(EGDMA-MAPA-VI)] film deposition on a SPR sensor with improved efficiency is described in this paper.

Research on Influencing Factors of Surface Plasmon Resonance Glucose Detection Based on D-Galactose/D-Glucose Protein Immobilizied by Amine Coupling Method

2010 ◽  
Author(s):  
Peng Wu ◽  
Dachao Li ◽  
Jingxin Zhang ◽  
Bing Song ◽  
Kexin Xu
Keyword(s):  
Mass Transfer ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Glucose Concentration ◽  
Interstitial Fluid ◽  
Transfer Effect ◽  
Sensor Surface ◽  
Mass Transfer Effect ◽  
Spr Sensor

Prediction of blood glucose based on measuring the glucose concentration in interstitial fluid is an effectively way to control diabetes. A surface plasmon resonance (SPR) system based on a miniature integrated SPR sensor is set up in this paper to measure the glucose concentration in interstitial fluid, and the D-galactose/D-glucose Binding Protein (GGBP) which can specifically absorb glucose is immobilized onto the SPR sensor surface by amine coupling method for higher sensitivity. The experiment result is affected by various factors, such as the grime on sensor surface, baseline, flow velocity, mass transfer effect, temperature, protein activity, bubbles. In this paper these factors are studied systemically by experiments and some solutions are proposed accordingly: (1) Make sure the sensor surface is clean before it is used. (2) Dynamic baseline can provide the best baseline location during the measurement. (3) Flow velocity of 10uL/min-30uL/min is supposed to be chosen. (4) The density of protein immobilized on the sensor surface should maintain lower to avoid the mass transfer effect. (5) Higher response is got when the temperature is between 26°C and 30°C and it is important to keep temperature constant during the experiment. (6) The protein should be preserved with a proper way and make sure it is active during the measurement. (7) The sample should be degassed and filtered before the experiment and make sure there is no air in the pipeline. Through optimizing the experiment conditions, the sensitivity and stability of the measurement are improved.

Surface Plasmon Resonance Signal Amplification Using Secondary Antibody Interaction for Illegal Compound Detection

2020 ◽  
Vol 845 ◽  
pp. 103-108
Author(s):  
Suherman ◽  
Dulal Chandra Kabiraz ◽  
Kinichi Morita ◽  
Toshikazu Kawaguchi
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Competitive Inhibition ◽  
Coupling Reaction ◽  
Primary Antibody ◽  
Antibody Detection ◽  
Secondary Antibody ◽  
Sensor Surface ◽  
Spr Sensor

The development of highly selective and sensitive surface plasmon resonance (SPR) immunoassay for the rapid detection of illegal compound using secondary antibody interaction labelled nanoparticle was conducted. For the construction of SPR sensor surface, the illegal compound (clenbuterol) was immobilized as antigen onto gold succinimide-terminated monolayer to perform amide-coupling reaction. In order to avoid non-specific reaction, the blocking agent (ethanol amine) was injected to the SPR system. Furthermore, indirect competitive inhibition method was employed in the detection of clenbuterol. In this work, the antibody solution (PBS solution containing clenbuterol antibody) premixed with a sample solution (PBS solution containing antigen) before the injection into the sensing system. As the premixed solution flowed over the sensor surface, the SPR senses the dielectric constant change at the interface due to the binding of the unreacted primary antibody to antigen-immobilized on the sensor surface. After this primary antibody detection, secondary antibody was injected to the SPR sensor surface. Here, we compared the signal difference of secondary antibody injection labelled Au nanoparticles (d = 40 nm). For the regeneration of the sensor surface, 0.1 M NaOH was used, so primary and secondary antibodies could be detached from the sensor surface. According to the indirect competitive inhibition method, it was found that the sensitivity for clenbuterol detection was enhanced from 2.5 ppt to 0.07 ppt.

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