scholarly journals Sensitive and Quantitative Detection of Anti-Poly(ethylene glycol) (PEG) Antibodies by Methoxy-PEG-Coated Surface Plasmon Resonance Sensors

2017 ◽  
Vol 89 (16) ◽  
pp. 8217-8222 ◽  
Author(s):  
Peng Zhang ◽  
Fang Sun ◽  
Hsiang-Chieh Hung ◽  
Priyesh Jain ◽  
Kasey Joanne Leger ◽  
...  
The Analyst ◽  
2014 ◽  
Vol 139 (16) ◽  
pp. 3977-3985 ◽  
Author(s):  
Thangavel Lakshmipriya ◽  
Yukichi Horiguchi ◽  
Yukio Nagasaki

In order to detect a low amount of human coagulation factor IX (FIX), poly(ethylene glycol) (PEG)/aptamer co-immobilized surface was constructed using original PEG–polyamine on surface plasmon resonance (SPR) sensor chip.


2014 ◽  
Vol 10 ◽  
pp. 1672-1680 ◽  
Author(s):  
Silvia Bernardi ◽  
Paola Fezzardi ◽  
Gabriele Rispoli ◽  
Stefania E Sestito ◽  
Francesco Peri ◽  
...  

Four novel calix[4]arene-based glycoclusters were synthesized by conjugating the saccharide units to the macrocyclic scaffold using the CuAAC reaction and using long and hydrophilic ethylene glycol spacers. Initially, two galactosylcalix[4]arenes were prepared starting from saccharide units and calixarene cores which differ in the relative dispositions of the alkyne and azido groups. Once the most convenient synthetic pathway was selected, two further lactosylcalix[4]arenes were obtained, one in the cone, the other one in the 1,3-alternate structure. Preliminary studies of the interactions of these novel glycocalixarenes with galectin-3 were carried out by using a lectin-functionalized chip and surface plasmon resonance. These studies indicate a higher affinity of lactosyl- over galactosylcalixarenes. Furthermore, we confirmed that in case of this specific lectin binding the presentation of lactose units on a cone calixarene is highly preferred with respect to its isomeric form in the 1,3-alternate structure.


2016 ◽  
Vol 55 (25) ◽  
pp. 6832 ◽  
Author(s):  
Zhaoyang Chen ◽  
Xin Zhao ◽  
Chengyou Lin ◽  
Shujing Chen ◽  
Liang Yin ◽  
...  

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