scholarly journals Binding assay for low molecular weight analytes based on reflectometry of absorbing molecules in porous substrates

The Analyst ◽  
2014 ◽  
Vol 139 (8) ◽  
pp. 1987-1992 ◽  
Author(s):  
Milena Stephan ◽  
Corinna Kramer ◽  
Claudia Steinem ◽  
Andreas Janshoff
Keyword(s):  
Molecular Weight ◽  
Small Molecule ◽  
Binding Assay ◽  
Pharmaceutical Research ◽  
Low Molecular Weight ◽  
Porous Substrates

Small molecule sensing is of great importance in pharmaceutical research.

Interactions governing the entrapment of anticancer drugs by low-molecular-weight hydrogelator for drug delivery applications

RSC Advances ◽  
2016 ◽  
Vol 6 (24) ◽  
pp. 19751-19757 ◽  
Author(s):  
Siddhi Gupta ◽  
Manish Singh ◽  
Amarendar Reddy M. ◽  
Prabhu S. Yavvari ◽  
Aasheesh Srivastava ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Molecular Weight ◽  
Anticancer Drugs ◽  
Small Molecule ◽  
Low Molecular Weight ◽  
Drug Encapsulation ◽  
Drug Delivery Applications

We present the effect of size, charge, and hydrophobicity of anticancer drugs on their drug encapsulation efficacy in anl-alanine-based small-molecule hydrogelator.

scholarly journals A Sensitive Aβ Oligomerization Assay for Identification of Small Molecule Inhibitors

2009 ◽  
Vol 3 (1) ◽  
pp. 108-116 ◽  
Author(s):  
Amanda M. Gonzales ◽  
Robert A. Orlando
Keyword(s):  
Molecular Weight ◽  
Small Molecule ◽  
Small Molecule Inhibitors ◽  
Early Stage ◽  
Synaptic Function ◽  
Low Molecular Weight ◽  
Aβ Peptide ◽  
Aβ Oligomers ◽  
Aβ Aggregation ◽  
Aβ Oligomerization

Amyloid deposits found in Alzheimer’s disease result from aggregation of Aβ peptide which leads to loss of synaptic function, chronic microglial activation and cognitive impairment. Because of this, identification of small molecule inhibitors of Aβ aggregation as potential therapeutics is a topic of current interest. The majority of inhibitor screening approaches rely on in vitro assays that lack the necessary sensitivity to distinguish low-molecular weight Aβ oligomers from larger, more advanced-stage fibrillar structures. Differentiating between these two structures is of vital concern since recent studies indicate that small, early-stage Aβ oligomers are the most neurotoxic form of peptide aggregate. To address this limitation, we have explored the adaptability of a recently described ELISA-based assay for discovery of small molecule inhibitors of Aβ oligomerization. Results show that this assay is highly sensitive as it is able to quantify Aβ oligomers with as little as 80 nM input peptide. In addition, data were obtained re-confirming the function of curcumin as a potent inhibitor of Aβ aggregation (IC50 = 2 μM) and defining its inhibitor:peptide functional stoichiometry. Further examination of other known anti-aggregation compounds showed that this assay is able to discriminate between inhibitors of early-stage, low-molecular weight oligomers and later-stage, high-molecular weight fibrillar structures. These findings indicate that this new ELISA-based assay is capable of identifying novel small molecule inhibitors that function during the initial stages of Aβ peptide assembly.

Unexpected room-temperature phosphorescence from a non-aromatic, low molecular weight, pure organic molecule through the intermolecular hydrogen bond

2018 ◽  
Vol 2 (11) ◽  
pp. 2124-2129 ◽  
Author(s):  
Manman Fang ◽  
Jie Yang ◽  
Xueqin Xiang ◽  
Yujun Xie ◽  
Yongqiang Dong ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Hydrogen Bond ◽  
Small Molecule ◽  
Organic Molecule ◽  
Room Temperature ◽  
Intermolecular Hydrogen Bond ◽  
Low Molecular Weight ◽  
Cyanoacetic Acid ◽  
Room Temperature Phosphorescence

The first example of a pure non-aromatic organic small molecule, cyanoacetic acid (CAA), with unexpected persistent RTP behavior is reported, which possesses the RTP lifetime as long as 862 ms.

A degradable low molecular weight monomer system with lower critical solution temperature behaviour in water

2019 ◽  
Vol 55 (6) ◽  
pp. 782-785 ◽  
Author(s):  
Bo Zheng ◽  
Zheng Luo ◽  
Yan Deng ◽  
Qiao Zhang ◽  
Lingyan Gao ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Crown Ether ◽  
Small Molecule ◽  
Lower Critical Solution Temperature ◽  
Solution Temperature ◽  
Low Molecular Weight ◽  
Temperature Behaviour ◽  
Critical Solution Temperature

A small molecule system showing LCST was prepared and the degradation of the crown ether motif effectively altered the thermo-responsiveness.

scholarly journals Small molecule-mediated co-assembly of amyloid-β oligomers reduces neurotoxicity through promoting non-fibrillar aggregation

2020 ◽  
Vol 11 (27) ◽  
pp. 7158-7169 ◽  
Author(s):  
Hao Liu ◽  
Chengyuan Qian ◽  
Tao Yang ◽  
Yanqing Wang ◽  
Jian Luo ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Blood Brain Barrier ◽  
Small Molecule ◽  
Rational Design ◽  
Amyloid Β ◽  
Low Molecular Weight ◽  
Aβ Oligomers ◽  
Barrier Permeability ◽  
Blood Brain Barrier Permeability ◽  
Brain Barrier Permeability

A rational design of pincer-like scaffold-based small molecule with blood-brain barrier permeability that can specifically co-assemble with low molecular weight Aβ oligomers to form non-fibrillar, degradable, non-toxic co-aggregates.

scholarly journals Near-infrared benzodiazoles as small molecule environmentally-sensitive fluorophores

2021 ◽  
Author(s):  
Fabio de Moliner ◽  
Ina Biazruchka ◽  
Karolina Konsewicz ◽  
Sam Benson ◽  
Suraj Singh ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Small Molecule ◽  
Near Infrared ◽  
Aqueous Media ◽  
Biological Imaging ◽  
Low Molecular Weight ◽  
Synthetic Approach ◽  
Spectral Window ◽  
Infrared Spectral ◽  
Environmentally Sensitive

AbstractThe development of fluorophores emitting in the near-infrared spectral window has gained increased attention given their suitable features for biological imaging. In this work, we have optimised a general and straightforward synthetic approach to prepare a small library of near-infrared-emitting C-bridged nitrobenzodiazoles using commercial precursors. C-bridged benzodiazoles have low molecular weight and neutral character as important features that are not common in most near-infrared dyes. We have investigated their fluorescence response in the presence of a wide array of 60 different biomolecules and identified compound 3i as a potential chemosensor to discriminate between Fe2+ and Fe3+ ions in aqueous media.

Studies on circulating immune complexes of the liver disease. 3. Conditions for Clq binding assay and low molecular weight Clq reactants

1980 ◽  
Vol 15 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Terukatsu Arima ◽  
Jin Narumoto ◽  
Hiroyuki Shimomura ◽  
Kinichiro Suwaki ◽  
Kensuke Kunishi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Liver Disease ◽  
Immune Complexes ◽  
Binding Assay ◽  
Circulating Immune Complexes ◽  
Low Molecular Weight

scholarly journals Liver enzyme induction by 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) is accompanied by an increase in the specific activity of elongation factor 1

1978 ◽  
Vol 172 (3) ◽  
pp. 479-486 ◽  
Author(s):  
E T Young ◽  
D M Nicholls
Keyword(s):  
Molecular Weight ◽  
Enzyme Induction ◽  
Sucrose Gradient ◽  
Binding Assay ◽  
Specific Activity ◽  
Elongation Factor ◽  
Low Molecular Weight ◽  
Binding Factor ◽  
The Difference ◽  
Elongation Factor 1

Homogenates of liver were obtained from control rats and from rats that had received DDT [1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane]. The postmicrosomal supernatant fractions were used for the purification of elongation factor 1 by hydroxyapatite chromatography and phosphocellulose chromarography. The amount of binding factor present was essentially the same for both groups of animals, but the specific activity, as measured by the binding assay, was about twice as high in the DDT-treated preparations. After sucrose-gradient sedimentation, the difference in specific activity was found to reside in the low-molecular-weight (50000) form of elongation factor 1. The implications of an increased reactivity of elongation factor 1 during the induction of membrane enzymes are discussed.

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