Studies on circulating immune complexes of the liver disease. 3. Conditions for Clq binding assay and low molecular weight Clq reactants

1980 ◽  
Vol 15 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Terukatsu Arima ◽  
Jin Narumoto ◽  
Hiroyuki Shimomura ◽  
Kinichiro Suwaki ◽  
Kensuke Kunishi ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Liver Disease ◽  
Immune Complexes ◽  
Binding Assay ◽  
Circulating Immune Complexes ◽  
Low Molecular Weight

scholarly journals The influence of the Selenium and Germanium on humoral immunity content in quails

2020 ◽  
pp. 88-95
Author(s):  
A. Yemelyanenko ◽  
S. Shmayun ◽  
M. Nishmenenko ◽  
O. Yemelyanenko ◽  
O. Poroshinska ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Immune System ◽  
Key Words ◽  
Humoral Immunity ◽  
Immune Complexes ◽  
Circulating Immune Complexes ◽  
Control Group ◽  
Low Molecular Weight ◽  
Main Indicator ◽  
Ig G

The results of the studies on the infl uence of Selenium and Germanium nanoacqualates complex on the content of immunoglobulins and circulating immune complexes in quail’s are presented in the article. It was found that the level of immunoglobulins is the main indicator of the state of humoral immunity. According to the results of the studies the content of immunoglobulins A, M, G in the quail’s serum was 0.80 ± 0.03 mg/ml, 0.30 ± 0.01 mg/ml, and 5.82 ± 0 , 06 mg/ml, accordingly. That are 31.1%, 20.0% and 6.0%, respectively, comparing with data of the control group. Inthe serum of fi ve days old quail’s Ig A content was 0.84 ± 0.02 mg/ml, Ig M 0.54 ± 0.03 mg/ml and Ig G 6.87 ± 0.04 mg/ml, that consisted 29.2%, 42.1% and 5.0%, respectively, comparing with the control group. The content of circulating immune complexes (CEC) in theblood serum of one- and fi ve-days old quails was 1.5 and 1.6 times, respectively lower than in the birds of the control group. The content of low molecular weight CECs in the serum of one- and fi ve-days old quail, decreased signifi cantly in both groups(in 2 and 1.1 times, respectively). This probably characterizes the overall effi cacy and balance of the immune system's responses in quails exposed to Selenium and Germanium nanoacqualates at used doses of 0.05 μg/kg and 5.0 μg/kg, respectively. We consider that as a positive eff ect on the immunity of quails in the early post-embryonic period. Therefore, the use of Selenium and Germanium nanoacqualates complex has a complementary eff ect and helps to increase the immunoreactivity of young animals by maintaining the activity of the immune system. Key words: Selenium nanoacquachelate, Germanium nanoacquachelate, quails, immunoglobulins, immunity.

Parallel Reduction of Plasma Levels of High and Low Molecular Weight Kininogen in Patients with Cirrhosis

1999 ◽  
Vol 82 (11) ◽  
pp. 1428-1432 ◽  
Author(s):  
Cheryl Scott ◽  
Francesco Salerno ◽  
Elettra Lorenzano ◽  
Werner Müller-Esterl ◽  
Angelo Agostoni ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Liver Disease ◽  
Liver Function ◽  
Plasma Levels ◽  
Plasma Concentrations ◽  
Normal Subjects ◽  
Low Molecular Weight ◽  
Major Band ◽  
Sds Page ◽  
Normal Controls

SummaryLittle is known about the regulation of high-molecular-weight-kininogen (HK) and low-molecular-weight-kininogen (LK) or the relationship of each to the degree of liver function impairment in patients with cirrhosis. In this study, we evaluated HK and LK quantitatively by a recently described particle concentration fluorescence immunoassay (PCFIA) and qualitatively by SDS PAGE and immunoblotting analyses in plasma from 33 patients with cirrhosis presenting various degrees of impairment of liver function. Thirty-three healthy subjects served as normal controls. Patients with cirrhosis had significantly lower plasma levels of HK (median 49 μg/ml [range 22-99 μg/ml]) and LK (58 μg/ml [15-100 μg/ml]) than normal subjects (HK 83 μg/ml [65-115 μg/ml]; LK 80 μg/ml [45-120 μg/ml]) (p < 0.0001). The plasma concentrations of HK and LK were directly related to plasma levels of cholinesterase (P < 0.0001) and albumin (P < 0.0001 and P < 0.001) and inversely to the Child-Pugh score (P < 0.0001) and to prothrombin time ratio (P < 0.0001) (reflecting the clinical and laboratory abnormalities in liver disease). Similar to normal individuals, in patients with cirrhosis, plasma HK and LK levels paralleled one another, suggesting that a coordinate regulation of those proteins persists in liver disease. SDS PAGE and immunoblotting analyses of kininogens in cirrhotic plasma showed a pattern similar to that observed in normal controls for LK (a single band at 66 kDa) with some lower molecular weight forms noted in cirrhotic plasma. A slight increase of cleavage of HK (a major band at 130 kDa and a faint but increased band at 107 kDa) was evident. The increased cleavage of HK was confirmed by the lower cleaved kininogen index (CKI), as compared to normal controls. These data suggest a defect in hepatic synthesis as well as increased destructive cleavage of both kininogens in plasma from patients with cirrhosis. The decrease of important regulatory proteins like kininogens may contribute to the imbalance in coagulation and fibrinolytic systems, which frequently occurs in cirrhotic patients.

scholarly journals Binding assay for low molecular weight analytes based on reflectometry of absorbing molecules in porous substrates

The Analyst ◽  
2014 ◽  
Vol 139 (8) ◽  
pp. 1987-1992 ◽  
Author(s):  
Milena Stephan ◽  
Corinna Kramer ◽  
Claudia Steinem ◽  
Andreas Janshoff
Keyword(s):  
Molecular Weight ◽  
Small Molecule ◽  
Binding Assay ◽  
Pharmaceutical Research ◽  
Low Molecular Weight ◽  
Porous Substrates

Small molecule sensing is of great importance in pharmaceutical research.

Enhancement of HBsAg detection in serum of patients with chronic liver disease following removal of circulating immune complexes

1994 ◽  
Vol 20 (3) ◽  
pp. 398-404 ◽  
Author(s):  
Zvi Ackerman ◽  
Jack R. Wands ◽  
Yair Gazitt ◽  
Christian Brechot ◽  
Michael C. Kew ◽  
...  
Keyword(s):  
Liver Disease ◽  
Chronic Liver Disease ◽  
Immune Complexes ◽  
Chronic Liver ◽  
Circulating Immune Complexes ◽  
Hbsag Detection

scholarly journals Circulating immune complexes in alcoholic liver disease

1978 ◽  
Vol 74 (5) ◽  
pp. 1075
Author(s):  
E. Penner ◽  
B. Albini ◽  
F. Milgrom
Keyword(s):  
Liver Disease ◽  
Alcoholic Liver Disease ◽  
Immune Complexes ◽  
Circulating Immune Complexes
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