A highly selective AIE fluorogen for lipid droplet imaging in live cells and green algae

2014 ◽  
Vol 2 (14) ◽  
pp. 2013-2019 ◽  
Author(s):  
Erjing Wang ◽  
Engui Zhao ◽  
Yuning Hong ◽  
Jacky W. Y. Lam ◽  
Ben Zhong Tang
Keyword(s):  
Green Algae ◽  
Lipid Droplet ◽  
Lipid Droplets ◽  
High Selectivity ◽  
High Specificity ◽  
Live Cells ◽  
Aggregation Induced Emission ◽  
Low Cytotoxicity ◽  
Excellent Photostability

An aggregation-induced emission fluorogen, TPE-AmAl, is reported to selectively image lipid droplets in live cells and green algae with high selectivity, high specificity, excellent photostability and low cytotoxicity.

The unusual aggregation-induced emission of coplanar organoboron isomers and their lipid droplet-specific applications

2018 ◽  
Vol 2 (8) ◽  
pp. 1498-1507 ◽  
Author(s):  
Jen-Shyang Ni ◽  
Haixiang Liu ◽  
Junkai Liu ◽  
Meijuan Jiang ◽  
Zheng Zhao ◽  
...  
Keyword(s):  
Excited State ◽  
Double Bond ◽  
Lipid Droplet ◽  
Lipid Droplets ◽  
Aggregation Induced Emission ◽  
Bond Reorganization

Organoboron isomers are not only rare coplanar AIEgens stemmed from excited-state double-bond reorganization, but also specifically target lipid droplets.

Facile construction of boranil complexes with aggregation-induced emission characteristics and their specific lipid droplet imaging applications

2019 ◽  
Vol 55 (58) ◽  
pp. 8494-8497 ◽  
Author(s):  
Na Zhao ◽  
Chengcheng Ma ◽  
Weiyao Yang ◽  
Wei Yin ◽  
Jiahui Wei ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Lipid Droplets ◽  
Living Cells ◽  
Emission Characteristics ◽  
Aggregation Induced Emission ◽  
Specific Lipid

A series of boranil complexes with aggregation-induced emission effects were facilely constructed, which can be utilized to image lipid droplets in living cells and yolk lipids in zebrafish.

scholarly journals Indolylbenzothiadiazoles as highly tunable fluorophores for imaging lipid droplet accumulation in astrocytes and glioblastoma cells

RSC Advances ◽  
2021 ◽  
Vol 11 (39) ◽  
pp. 23960-23967
Author(s):  
Kilian Colas ◽  
Karl O. Holmberg ◽  
Linus Chiang ◽  
Susanne Doloczki ◽  
Fredrik J. Swartling ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Lipid Droplets ◽  
Glioblastoma Cells ◽  
Photophysical Study

We present an extensive photophysical study of a series of fluorescent indolylbenzothiadiazole derivatives and their ability to specifically image lipid droplets in astrocytes and glioblastoma cells.

An aggregation-induced emission (AIE)-active fluorescent chemodosimeter for selective sensing of hypochlorite in water and solid state: Endogenous detection of hypochlorite in live cells

2021 ◽  
Vol 196 ◽  
pp. 109758
Author(s):  
Sandip Kumar Samanta ◽  
Kalipada Maiti ◽  
Saikat Kumar Manna ◽  
Syed Samim Ali ◽  
Uday Narayan Guria ◽  
...  
Keyword(s):  
Solid State ◽  
Live Cells ◽  
Aggregation Induced Emission ◽  
Fluorescent Chemodosimeter

Identification of Perilipin-2 as a lipid droplet protein regulated in oocytes during maturation

2010 ◽  
Vol 22 (8) ◽  
pp. 1262 ◽  
Author(s):  
Xing Yang ◽  
Kylie R. Dunning ◽  
Linda L.-Y. Wu ◽  
Theresa E. Hickey ◽  
Robert J. Norman ◽  
...  
Keyword(s):  
Lipid Droplet ◽  
Lipid Content ◽  
Lipid Droplets ◽  
Intracellular Lipids ◽  
Epidermal Growth ◽  
Novel Method ◽  
Perilipin 2 ◽  
Lipid Droplet Protein

Lipid droplet proteins regulate the storage and utilisation of intracellular lipids. Evidence is emerging that oocyte lipid utilisation impacts embryo development, but lipid droplet proteins have not been studied in oocytes. The aim of the present study was to characterise the size and localisation of lipid droplets in mouse oocytes during the periovulatory period and to identify lipid droplet proteins as potential biomarkers of oocyte lipid content. Oocyte lipid droplets, visualised using a novel method of staining cumulus–oocyte complexes (COCs) with BODIPY 493/503, were small and diffuse in oocytes of preovulatory COCs, but larger and more centrally located after maturation in response to ovulatory human chorionic gonadotrophin (hCG) in vivo, or FSH + epidermal growth factor in vitro. Lipid droplet proteins Perilipin, Perilipin-2, cell death-inducing DNA fragmentation factor 45-like effector (CIDE)-A and CIDE-B were detected in the mouse ovary by immunohistochemistry, but only Perilipin-2 was associated with lipid droplets in the oocyte. In COCs, Perilipin-2 mRNA and protein increased in response to ovulatory hCG. IVM failed to induce Perilipin-2 mRNA, yet oocyte lipid content was increased in this context, indicating that Perilipin-2 is not necessarily reflective of relative oocyte lipid content. Thus, Perilipin-2 is a lipid droplet protein in oocytes and its induction in the COC concurrent with dynamic reorganisation of lipid droplets suggests marked changes in lipid utilisation during oocyte maturation.

TICT based fluorescent probe with excellent photostability for real-time and long-term imaging of lipid droplets

2019 ◽  
Vol 60 (29) ◽  
pp. 1880-1884 ◽  
Author(s):  
Yunxia Li ◽  
Minjie Zhang ◽  
Xiaohui Chen ◽  
Jianshu Liang ◽  
Dongcheng Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Lipid Droplets ◽  
Excellent Photostability

scholarly journals HSD17B13: A Potential Therapeutic Target for NAFLD

2022 ◽  
Vol 8 ◽  
Author(s):  
Hai-bo Zhang ◽  
Wen Su ◽  
Hu Xu ◽  
Xiao-yan Zhang ◽  
You-fei Guan
Keyword(s):  
Liver Disease ◽  
Lipid Droplet ◽  
Lipid Droplets ◽  
Critical Role ◽  
Chronic Liver ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Nonalcoholic Fatty Liver ◽  
Promising Target ◽  
Associated Proteins

Nonalcoholic fatty liver disease (NAFLD), especially in its inflammatory form (steatohepatitis, NASH), is closely related to the pathogenesis of chronic liver disease. Despite substantial advances in the management of NAFLD/NASH in recent years, there are currently no efficacious therapies for its treatment. The biogenesis and expansion of lipid droplets (LDs) are critical pathophysiological processes in the development of NAFLD/NASH. In the past decade, increasing evidence has demonstrated that lipid droplet-associated proteins may represent potential therapeutic targets for the treatment of NAFLD/NASH given the critical role they play in regulating the biogenesis and metabolism of lipid droplets. Recently, HSD17B13, a newly identified liver-enriched, hepatocyte-specific, lipid droplet-associated protein, has been reported to be strongly associated with the development and progression of NAFLD/NASH in both mice and humans. Notably, human genetic studies have repeatedly reported a robust association of HSD17B13 single nucleotide polymorphisms (SNPs) with the occurrence and severity of NAFLD/NASH and other chronic liver diseases (CLDs). Here we briefly overview the discovery, tissue distribution, and subcellular localization of HSD17B13 and highlight its important role in promoting the pathogenesis of NAFLD/NASH in both experimental animal models and patients. We also discuss the potential of HSD17B13 as a promising target for the development of novel therapeutic agents for NAFLD/NASH.

Cellular membrane-anchored fluorescent probe with aggregation-induced emission characteristics for selective detection of Cu2+ ions

2017 ◽  
Vol 196 ◽  
pp. 377-393 ◽  
Author(s):  
Danni Liu ◽  
Shenglu Ji ◽  
Heran Li ◽  
Liang Hong ◽  
Deling Kong ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Cell Membrane ◽  
Water Solubility ◽  
Live Cells ◽  
Probe Design ◽  
Specific Cell ◽  
Selective Detection ◽  
High Concentration ◽  
Aggregation Induced Emission ◽  
Emissive Probes

The exploration of advanced fluorescent probes that can detect divalent copper (Cu2+) in aqueous environments and even in live organisms is particularly valuable for understanding the occurrence and development of Cu2+-related diseases. In this work, we report the design and synthesis of an aggregation-induced emission luminogen (AIEgen)-based probe (TPE-Py-EEGTIGYG) by integrating an AIEgen, TPE-Py, with a peptide, EEGTIGYG, which can selectively detect Cu2+ in both aqueous solution and live cells. Peptide EEGTIGYG has dual functionality in the probe design, namely improving water solubility and providing specific cell membrane-binding ability. TPE-Py-EEGTIGYG can self-assemble into nanoaggregates at high concentration in aqueous solution (e.g., 25 μM), which possess large fluorescence output due to the restriction of intramolecular rotation of the phenyl rings on TPE-Py. The fluorescence of the TPE-Py-EEGTIGYG nanoaggregates can be significantly quenched by Cu2+ but not by other metal ions, achieving the selective detection of Cu2+ in aqueous media. Furthermore, TPE-Py-EEGTIGYG can exist as a molecular species and is very weakly fluorescent in dilute aqueous solution (e.g., 5 μM), but can however largely switch on its fluorescence upon specifically anchoring onto the cell membrane. The emissive probes on the cell membrane can be used for the detection of Cu2+ ions that move in and out of cells with a fluorescence “turn-off” mode.

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