A near-infrared fluorescent sensor for selective detection of cysteine and its application in live cell imaging

RSC Advances ◽  
2014 ◽  
Vol 4 (16) ◽  
pp. 8360 ◽  
Author(s):  
Ying-Shi Guan ◽  
Li-Ya Niu ◽  
Yu-Zhe Chen ◽  
Li-Zhu Wu ◽  
Chen-Ho Tung ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Fluorescent Sensor ◽  
Live Cell ◽  
Selective Detection

A new ‘turn-on’ and reversible fluorescent sensor for Al3+ detection and live cell imaging

2019 ◽  
Vol 11 (43) ◽  
pp. 5598-5606 ◽  
Author(s):  
JianHua Wang ◽  
LiHen Feng ◽  
JianBin Chao ◽  
Yu Wang ◽  
ShaoMin Shuang
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Sensor ◽  
Live Cell ◽  
Selective Detection ◽  
Aqueous Methanol ◽  
Turn On

Herein, we report a new benzothiazole-based azomethine receptor L as a reversible fluorescent sensor for the selective detection of Al3+ in buffered aqueous methanol (20 mM HEPES, pH = 6.0, 50% (v/v) CH3OH).

Simple Fluorescence Turn-On Chemosensor for Selective Detection of Ba2+ Ion and Its Live Cell Imaging

2019 ◽  
Vol 91 (15) ◽  
pp. 10095-10101 ◽  
Author(s):  
Palanisamy Ravichandiran ◽  
Sivakumar Allur Subramaniyan ◽  
Antony Paulraj Bella ◽  
Princy Merlin Johnson ◽  
Ae Rhan Kim ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Selective Detection ◽  
Turn On ◽  
Fluorescence Turn On

A rhodamine-phosphonate off–on fluorescent sensor for Hg2+ in natural water and its application in live cell imaging

2013 ◽  
Vol 177 ◽  
pp. 997-1002 ◽  
Author(s):  
Di Zhang ◽  
Man Li ◽  
Min Wang ◽  
Jinhui Wang ◽  
Xian Yang ◽  
...  
Keyword(s):  
Natural Water ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Sensor ◽  
Live Cell

scholarly journals A Near‐Infrared Photoswitchable Protein–Fluorophore Tag for No‐Wash Live Cell Imaging

2018 ◽  
Vol 130 (49) ◽  
pp. 16315-16319 ◽  
Author(s):  
Wei Sheng ◽  
Setare Tahmasebi Nick ◽  
Elizabeth M. Santos ◽  
Xinliang Ding ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Live Cell

A new visible-light-excitable ICT-CHEF-mediated fluorescence ‘turn-on’ probe for the selective detection of Cd2+ in a mixed aqueous system with live-cell imaging

2015 ◽  
Vol 44 (12) ◽  
pp. 5763-5770 ◽  
Author(s):  
Shyamaprosad Goswami ◽  
Krishnendu Aich ◽  
Sangita Das ◽  
Chitrangada Das Mukhopadhyay ◽  
Deblina Sarkar ◽  
...  
Keyword(s):  
Visible Light ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Aqueous System ◽  
Live Cell ◽  
Selective Detection ◽  
Turn On ◽  
Fluorescence Turn On

A new quinoline based sensor was developed and applied for the selective detection of Cd2+ both in vitro and in vivo.

scholarly journals Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies

2021 ◽  
Vol 22 (20) ◽  
pp. 11092
Author(s):  
Magalie Bénard ◽  
Damien Schapman ◽  
Christophe Chamot ◽  
Fatéméh Dubois ◽  
Guénaëlle Levallet ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Live Cell Imaging ◽  
Near Infrared ◽  
Cell Imaging ◽  
Light Exposure ◽  
Photon Counting ◽  
Live Cell ◽  
Stimulated Emission ◽  
Plot Analysis ◽  
Phasor Plot

Fluorescence microscopy is essential for a detailed understanding of cellular processes; however, live-cell preservation during imaging is a matter of debate. In this study, we proposed a guide to optimize advanced light microscopy approaches by reducing light exposure through fluorescence lifetime (τ) exploitation of red/near-infrared dyes. Firstly, we characterized key instrumental elements which revealed that red/near-infrared laser lines with an 86x (Numerical Aperture (NA) = 1.2, water immersion) objective allowed high transmission of fluorescence signals, low irradiance and super-resolution. As a combination of two technologies, i.e., vacuum tubes (e.g., photomultiplier) and semiconductor microelectronics (e.g., avalanche photodiode), type S, X and R of hybrid detectors (HyD-S, HyD-X and HyD-R) were particularly adapted for red/near-infrared photon counting and τ separation. Secondly, we tested and compared lifetime-based imaging including coarse τ separation for confocal microscopy, fitting and phasor plot analysis for fluorescence lifetime microscopy (FLIM), and lifetimes weighting for enhanced stimulated emission depletion (STED) nanoscopy, in light of red/near-infrared multiplexing. Mainly, we showed that the choice of appropriate imaging approach may depend on fluorochrome number, together with their spectral/lifetime characteristics and STED compatibility. Photon-counting mode and sensitivity of HyDs together with phasor plot analysis of fluorescence lifetimes enabled the flexible and fast imaging of multi-labeled living H28 cells. Therefore, a combination of red/near-infrared dyes labeling with lifetime-based strategies offers new perspectives for live-cell imaging by enhancing sample preservation through acquisition time and light exposure reduction.

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