Hybrid assembly of DNA-coated gold nanoparticles with water soluble conjugated polymers for studying protein–DNA interaction and ligand inhibition

RSC Advances ◽  
2014 ◽  
Vol 4 (17) ◽  
pp. 8883 ◽  
Author(s):  
Steven Lukman ◽  
Khin Moh Moh Aung ◽  
Michelle Gek Liang Lim ◽  
Shuzhen Hong ◽  
Si Kee Tan ◽  
...  
2020 ◽  
Vol 16 (7) ◽  
pp. 905-913
Author(s):  
Youyuan Peng ◽  
Qingshan Miao

Background: L-Ascorbic acid (AA) is a kind of water soluble vitamin, which is mainly present in fruits, vegetables and biological fluids. As a low cost antioxidant and effective scavenger of free radicals, AA may help to prevent diseases such as cancer and Parkinson’s disease. Owing to its role in the biological metabolism, AA has also been utilized for the therapy of mental illness, common cold and for improving the immunity. Therefore, it is very necessary and urgent to develop a simple, rapid and selective strategy for the detection of AA in various samples. Methods: The molecularly imprinted poly(o-phenylenediamine) (PoPD) film was prepared for the analysis of L-ascorbic acid (AA) on gold nanoparticles (AuNPs) - multiwalled carbon nanotubes (MWCNTs) modified glass carbon electrode (GCE) by electropolymerization of o-phenylenediamine (oPD) and AA. Experimental parameters including pH value of running buffer and scan rates were optimized. Scanning electron microscope (SEM), fourier-transform infrared (FTIR) spectra, cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were utilized for the characterization of the imprinted polymer film. Results: Under the selected experimental conditions, the DPV peak currents of AA exhibit two distinct linear responses ranging from 0.01 to 2 μmol L-1 and 2 to 100 μmol L-1 towards the concentrations of AA, and the detection limit was 2 nmol L-1 (S/N=3). Conclusion: The proposed electrochemical sensor possesses excellent selectivity for AA, along with good reproducibility and stability. The results obtained from the analysis of AA in real samples demonstrated the applicability of the proposed sensor to practical analysis.


Author(s):  
Turgut Keleş ◽  
Burak Barut ◽  
Sercan Yıldırım ◽  
Can Özgür Yalçın ◽  
Zekeriya Biyiklioglu

2011 ◽  
Vol 415-417 ◽  
pp. 617-620 ◽  
Author(s):  
Yan Su ◽  
Ying Yun Lin ◽  
Yu Li Fu ◽  
Fan Qian ◽  
Xiu Pei Yang ◽  
...  

Water-soluble gold nanoparticles (AuNPs) were prepared using 2-mercapto-4-methyl-5- thiazoleacetic acid (MMTA) as a stabilizing agent and sodium borohydride (NaBH4) as a reducing agent. The AuNPs product was analyzed by transmission electron microscopy (TEM), UV-vis absorption spectroscopy and Fourier transform infrared spectroscopy (FTIR). The TEM image shows that the particles were well-dispersed and their average particle size is about 5 nm. The UV-vis absorption and FTIR spectra confirm that the MMTA-AuNPs was stabilized by the carboxylate ions present on the surface of the AuNPs.


2007 ◽  
Vol 90 (26) ◽  
pp. 263503 ◽  
Author(s):  
Wonjoo Lee ◽  
Rajaram S. Mane ◽  
Sun-Ki Min ◽  
Tae Hyun Yoon ◽  
Sung-Hwan Han ◽  
...  

Cellulose ◽  
2011 ◽  
Vol 18 (4) ◽  
pp. 929-936 ◽  
Author(s):  
Yukiko Enomoto-Rogers ◽  
Hiroshi Kamitakahara ◽  
Arata Yoshinaga ◽  
Toshiyuki Takano

2009 ◽  
pp. 4278 ◽  
Author(s):  
Andrew J. Hallett ◽  
Paul Christian ◽  
Jennifer E. Jones ◽  
Simon J. A. Pope

Author(s):  
Thi Lanh Le ◽  
Quang Khieu Dinh ◽  
Thai Hoa Tran ◽  
Hai Phong Nguyen ◽  
Thi Le Hien Hoang ◽  
...  

2017 ◽  
Vol 46 (27) ◽  
pp. 8736-8745 ◽  
Author(s):  
Cornelia E. Peña-González ◽  
Elzbieta Pedziwiatr-Werbicka ◽  
Dzmitry Shcharbin ◽  
Carlos Guerrero-Beltrán ◽  
Viktar Abashkin ◽  
...  

Water soluble gold nanoparticles have been isolated with pendant cationic carbosilane dendrons and their biological properties have been evaluated.


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