Chemical interactions of mercury species and some transition and noble metals towards metallothionein (Zn7MT-2) evaluated using SEC/ICP-MS, RP-HPLC/ESI-MS and MALDI-TOF-MS

Metallomics ◽  
2013 ◽  
Vol 5 (7) ◽  
pp. 855 ◽  
Author(s):  
Ming Xu ◽  
Limin Yang ◽  
Qiuquan Wang
Keyword(s):  
Noble Metals ◽  
Mercury Species ◽  
Chemical Interactions ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Rp Hplc ◽  
Icp Ms ◽  
Tof Ms

NMR, HPLC-ESI-MS, and MALDI-TOF MS Analysis of Condensed Tannins from Delonix regia (Bojer ex Hook.) Raf. and Their Bioactivities

2012 ◽  
Vol 60 (19) ◽  
pp. 5013-5022 ◽  
Author(s):  
Wei-Ming Chai ◽  
Yan Shi ◽  
Hui-Ling Feng ◽  
Ling Qiu ◽  
Hai-Chao Zhou ◽  
...  
Keyword(s):  
Condensed Tannins ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Delonix Regia ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

scholarly journals Development of an Optimized MALDI-TOF-MS Method for High-Throughput Identification of High-Molecular-Weight Glutenin Subunits in Wheat

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4347
Author(s):  
You-Ran Jang ◽  
Kyoungwon Cho ◽  
Se Won Kim ◽  
Susan B. Altenbach ◽  
Sun-Hyung Lim ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
High Throughput ◽  
Glutenin Subunits ◽  
Maldi Tof Ms ◽  
Molecular Weights ◽  
Maldi Tof ◽  
Rp Hplc ◽  
Group 2 ◽  
Tof Ms

Because high-molecular-weight glutenin subunits (HMW-GS) are important contributors to wheat end-use quality, there is a need for high-throughput identification of HMW-GS in wheat genetic resources and breeding lines. We developed an optimized method using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to distinguish individual HMW-GS by considering the effects of the alkylating reagent in protein extraction, solvent components, dissolving volume, and matrix II components. Using the optimized method, 18 of 22 HMW-GS were successfully identified in standard wheat cultivars by differences in molecular weights or by their associations with other tightly linked subunits. Interestingly, 1Bx7 subunits were divided into 1Bx7 group 1 and 1Bx7 group 2 proteins with molecular weights of about 82,400 and 83,000 Da, respectively. Cultivars containing the 1Bx7 group 2 proteins were distinguished from those containing 1Bx7OE using well-known DNA markers. HMW-GS 1Ax2* and 1Bx6 and 1By8 and 1By8*, which are difficult to distinguish due to very similar molecular weights, were easily identified using RP-HPLC. To validate the method, HMW-GS from 38 Korean wheat varieties previously evaluated by SDS-PAGE combined with RP-HPLC were analyzed by MALDI-TOF-MS. The optimized MALDI-TOF-MS method will be a rapid, high-throughput tool for selecting lines containing desirable HMW-GS for breeding efforts.

Subunit Characteristics of Pig Pancreas Ferritin Revealed by MALDI-TOF MS and RP-HPLC

2008 ◽  
Vol 24 (5) ◽  
pp. 550-556 ◽  
Author(s):  
L HUANG ◽  
Z LIN ◽  
Q LIN ◽  
L LUO ◽  
H HUANG
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Rp Hplc ◽  
Tof Ms

Ellagitannin Composition of Blackberry As Determined by HPLC-ESI-MS and MALDI-TOF-MS

2008 ◽  
Vol 56 (3) ◽  
pp. 661-669 ◽  
Author(s):  
Tiffany J. Hager ◽  
Luke R. Howard ◽  
Rohana Liyanage ◽  
Jackson O. Lay ◽  
Ronald L. Prior
Keyword(s):  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Comparison of MALDI-TOF-MS and RP-HPLC as Rapid Screening Methods for Wheat Lines With Altered Gliadin Compositions

2020 ◽  
Vol 11 ◽  
Author(s):  
You-Ran Jang ◽  
Kyoungwon Cho ◽  
Sewon Kim ◽  
Jae-Ryeong Sim ◽  
Su-Bin Lee ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Mutation Breeding ◽  
Rapid Screening ◽  
Food Allergies ◽  
Screening Methods ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Rp Hplc ◽  
Wheat Lines ◽  
Tof Ms

The wheat gliadins are a complex group of flour proteins that can trigger celiac disease and serious food allergies. As a result, mutation breeding and biotechnology approaches are being used to develop new wheat lines with reduced immunogenic potential. Key to these efforts is the development of rapid, high-throughput methods that can be used as a first step in selecting lines with altered gliadin contents. In this paper, we optimized matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and reversed-phase high-performance liquid chromatography (RP-HPLC) methods for the separation of gliadins from Triticum aestivum cv. Chinese Spring (CS). We evaluated the quality of the resulting profiles using the complete set of gliadin gene sequences recently obtained from this cultivar as well as a set of aneuploid lines in CS. The gliadins were resolved into 13 peaks by MALDI-TOF-MS. α- or γ-gliadins that contain abundant celiac disease epitopes and are likely targets for efforts to reduce the immunogenicity of flour were found in several peaks. However, other peaks contained multiple α- and γ-gliadins, including one peak with as many as 12 different gliadins. In comparison, separation of proteins by RP-HPLC yielded 28 gliadin peaks, including 13 peaks containing α-gliadins and eight peaks containing γ-gliadins. While the separation of α- and γ-gliadins gliadins achieved by RP-HPLC was better than that achieved by MALDI-TOF-MS, it was not possible to link peaks with individual protein sequences. Both MALDI-TOF-MS and RP-HPLC provided adequate separation of ω-gliadins. While MALDI-TOF-MS is faster and could prove useful in studies that target specific gliadins, RP-HPLC is an effective method that can be applied more broadly to detect changes in gliadin composition.

Determination of mycotoxins by HPLC, LC-ESI-MS/MS, and MALDI-TOF MS in Fusarium species-infected sugarcane

2018 ◽  
Vol 123 ◽  
pp. 98-110 ◽  
Author(s):  
John Martin Jerome Jeyakumar ◽  
Muqing Zhang ◽  
Muthu Thiruvengadam
Keyword(s):  
Fusarium Species ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Tof Ms

Comparison of MALDI-TOF-MS and HPLC-ESI-MS/MS for Endopeptidase Activity-Based Quantification of Anthrax Lethal Factor in Serum

2011 ◽  
Vol 83 (5) ◽  
pp. 1760-1765 ◽  
Author(s):  
Zsuzsanna Kuklenyik ◽  
Anne E. Boyer ◽  
Renato Lins ◽  
Conrad P. Quinn ◽  
Maribel Gallegos-Candela ◽  
...  
Keyword(s):  
Lethal Factor ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Anthrax Lethal Factor ◽  
Endopeptidase Activity ◽  
Tof Ms

Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)

2018 ◽  
Vol 143 ◽  
pp. 457-466 ◽  
Author(s):  
Ivana K. Levy ◽  
Ricardo Neme Tauil ◽  
Maria P. Valacco ◽  
Silvia Moreno ◽  
Gabriela Siracusano ◽  
...  
Keyword(s):  
18Th Century ◽  
Mural Painting ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Tof Ms
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