Comparison of MALDI-TOF-MS and HPLC-ESI-MS/MS for Endopeptidase Activity-Based Quantification of Anthrax Lethal Factor in Serum

2011 ◽  
Vol 83 (5) ◽  
pp. 1760-1765 ◽  
Author(s):  
Zsuzsanna Kuklenyik ◽  
Anne E. Boyer ◽  
Renato Lins ◽  
Conrad P. Quinn ◽  
Maribel Gallegos-Candela ◽  
...  
Keyword(s):  
Lethal Factor ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Anthrax Lethal Factor ◽  
Endopeptidase Activity ◽  
Tof Ms

scholarly journals Validated MALDI-TOF-MS method for anthrax lethal factor provides early diagnosis and evaluation of therapeutics

2018 ◽  
Vol 543 ◽  
pp. 97-107 ◽  
Author(s):  
Maribel Gallegos-Candela ◽  
Anne E. Boyer ◽  
Adrian R. Woolfitt ◽  
Judy Brumlow ◽  
Renato C. Lins ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Lethal Factor ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Anthrax Lethal Factor ◽  
Tof Ms

NMR, HPLC-ESI-MS, and MALDI-TOF MS Analysis of Condensed Tannins from Delonix regia (Bojer ex Hook.) Raf. and Their Bioactivities

2012 ◽  
Vol 60 (19) ◽  
pp. 5013-5022 ◽  
Author(s):  
Wei-Ming Chai ◽  
Yan Shi ◽  
Hui-Ling Feng ◽  
Ling Qiu ◽  
Hai-Chao Zhou ◽  
...  
Keyword(s):  
Condensed Tannins ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Delonix Regia ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Ellagitannin Composition of Blackberry As Determined by HPLC-ESI-MS and MALDI-TOF-MS

2008 ◽  
Vol 56 (3) ◽  
pp. 661-669 ◽  
Author(s):  
Tiffany J. Hager ◽  
Luke R. Howard ◽  
Rohana Liyanage ◽  
Jackson O. Lay ◽  
Ronald L. Prior
Keyword(s):  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Tof Ms

Determination of mycotoxins by HPLC, LC-ESI-MS/MS, and MALDI-TOF MS in Fusarium species-infected sugarcane

2018 ◽  
Vol 123 ◽  
pp. 98-110 ◽  
Author(s):  
John Martin Jerome Jeyakumar ◽  
Muqing Zhang ◽  
Muthu Thiruvengadam
Keyword(s):  
Fusarium Species ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Tof Ms

Investigation of proteins in samples of a mid-18th century colonial mural painting by MALDI-TOF/MS and LC-ESI/MS (Orbitrap)

2018 ◽  
Vol 143 ◽  
pp. 457-466 ◽  
Author(s):  
Ivana K. Levy ◽  
Ricardo Neme Tauil ◽  
Maria P. Valacco ◽  
Silvia Moreno ◽  
Gabriela Siracusano ◽  
...  
Keyword(s):  
18Th Century ◽  
Mural Painting ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Tof Ms

Analysis of human histone H4 by capillary electrophoresis in a pullulan-coated capillary, LC-ESI-MS and MALDI-TOF-MS

2008 ◽  
Vol 390 (7) ◽  
pp. 1881-1888 ◽  
Author(s):  
Stefano Olmo ◽  
Roberto Gotti ◽  
Marina Naldi ◽  
Vincenza Andrisano ◽  
Natalia Calonghi ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Histone H4 ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Tof Ms

In situ generation of milk protein-derived peptides in drying-off cows

2010 ◽  
Vol 77 (4) ◽  
pp. 487-497 ◽  
Author(s):  
Chin H Ho ◽  
Chai J Chang ◽  
Wen B Liu ◽  
Huo C Peh ◽  
Shuen E Chen ◽  
...  
Keyword(s):  
Milk Protein ◽  
Peptide Sequence ◽  
Functional Screening ◽  
Post Translational Modification ◽  
Peptide Profile ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Maldi Tof ◽  
Tof Ms

Our previous studies demonstrated prompt elevation of proteinase activity in mammary secretion of drying-off cows and goats. The current study examined the progressive changes in composition of cow mammary secretion following drying-off and, in parallel, characterized the mode of peptide neogenesis using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography-electrospray-ionization (LC-ESI) MS/MS. The results show that the percentage of casein of total milk protein at time of drying-off was 76%, which dropped to 41%, 24%, and 16%, respectively, 1, 2, and 3 weeks after drying-off. Levels of β-lactoglobulin and α-lactoalbumin in mammary secretions of drying-off cows decreased prominently while levels of lactoferrin, BSA, and casein derived-proteins increased concomitantly compared with regular milk. A fractionation procedure was applied to remove molecules larger than 10 kDa before MALDI-TOF MS and LC-ESI MS/MS and the results show that the MALDI-TOF MS peptide profile of mammary secretion ranging from m/z 600 to 4000 was apparently modified after drying-off for 1 week, whereas species 1590 m/z and 2460 m/z were most obviously enriched compared with regular milk. LC-ESI MS/MS results were used to map peptide sequence with Mascot search server and under no post translational modification to reduce database size and 202 novel β-casein-derived peptides were successfully identified in mammary secretion after drying-off for 1 week in contrast to regular milk. Accordingly at least 48 additional cleavage positions were assigned on β-casein for mammary secretion. Among the 202 novel peptides, 5 are homologous with confirmed opioid agonists, angiotensin 1-converting enzyme inhibitors, or immuno-modulators. In conclusion, peptides are released in situ from milk proteins within short intervals following drying-off in cows. They might play roles in the transition of mammary glands from lactating to non-lactating. With specified post-translational modifications and focused functional screening, novel peptides are yet to be discovered in dry cow mammary secretion.

Sign in / Sign up

Export Citation Format

Share Document