Technologies for label-free separation of circulating tumor cells: from historical foundations to recent developments

Lab on a Chip ◽  
2014 ◽  
Vol 14 (1) ◽  
pp. 32-44 ◽  
Author(s):  
Chao Jin ◽  
Sarah M. McFaul ◽  
Simon P. Duffy ◽  
Xiaoyan Deng ◽  
Peyman Tavassoli ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Label Free ◽  
Recent Developments

scholarly journals 56P Single circulating tumor cells RNA profiling by label-free enrichment and active single cell selection on an integrated fluidic system

2016 ◽  
Vol 27 ◽  
pp. ix15-ix16
Author(s):  
Y.F. Lee ◽  
N. Ramalingam ◽  
L. Szpankowski ◽  
A. Leyrat ◽  
N.D. Angeles ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cell Selection ◽  
Label Free ◽  
Rna Profiling ◽  
Free Enrichment

scholarly journals Label-free enumeration, collection and downstream cytological and cytogenetic analysis of circulating tumor cells

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Manjima Dhar ◽  
Edward Pao ◽  
Corinne Renier ◽  
Derek E. Go ◽  
James Che ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cytogenetic Analysis ◽  
Label Free

A Chemical Method for Specific Capture of Circulating Tumor Cells Using Label-Free Polyphenol-Functionalized Films

2018 ◽  
Vol 30 (13) ◽  
pp. 4372-4382 ◽  
Author(s):  
Liwei Yang ◽  
He Sun ◽  
Wenning Jiang ◽  
Ting Xu ◽  
Bing Song ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Chemical Method ◽  
Label Free ◽  
Functionalized Films

scholarly journals Perspectives on liquid biopsy for label‐free detection of “circulating tumor cells” through intelligent lab‐on‐chips

View ◽  
2020 ◽  
Vol 1 (3) ◽  
pp. 20200034 ◽  
Author(s):  
Lisa Miccio ◽  
Flora Cimmino ◽  
Ivana Kurelac ◽  
Massimiliano M. Villone ◽  
Vittorio Bianco ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Label Free ◽  
Label Free Detection

scholarly journals Dysmetabolic Circulating Tumor Cells Are Prognostic in Metastatic Breast Cancer

Cancers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1005 ◽  
Author(s):  
Giulia Brisotto ◽  
Eva Biscontin ◽  
Elisabetta Rossi ◽  
Michela Bulfoni ◽  
Aigars Piruska ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Metastatic Breast Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Metastatic Breast ◽  
Therapy Response ◽  
Label Free ◽  
Breast Cancer Patients ◽  
Combining Data ◽  
Definition Of

Circulating tumor cells (CTCs) belong to a heterogeneous pool of rare cells, and a unequivocal phenotypic definition of CTC is lacking. Here, we present a definition of metabolically-altered CTC (MBA-CTCs) as CD45-negative cells with an increased extracellular acidification rate, detected with a single-cell droplet microfluidic technique. We tested the prognostic value of MBA-CTCs in 31 metastatic breast cancer patients before starting a new systemic therapy (T0) and 3–4 weeks after (T1), comparing results with a parallel FDA-approved CellSearch (CS) approach. An increased level of MBA-CTCs was associated with: i) a shorter median PFS pre-therapy (123 days vs. 306; p < 0.0001) and during therapy (139 vs. 266 days; p = 0.0009); ii) a worse OS pre-therapy (p = 0.0003, 82% survival vs. 20%) and during therapy (p = 0.0301, 67% survival vs. 38%); iii) good agreement with therapy response (kappa = 0.685). The trend of MBA-CTCs over time (combining data at T0 and T1) added information with respect to separate evaluation of T0 and T1. The combined results of the two assays (MBA and CS) increased stratification accuracy, while correlation between MBA and CS was not significant, suggesting that the two assays are detecting different CTC subsets. In conclusion, this study suggests that MBA allows detection of both EpCAM-negative and EpCAM-positive, viable and label-free CTCs, which provide clinical information apparently equivalent and complementary to CS. A further validation of proposed method and cut-offs is needed in a larger, separate study.

scholarly journals Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells

Lab on a Chip ◽  
2014 ◽  
Vol 14 (1) ◽  
pp. 128-137 ◽  
Author(s):  
Majid Ebrahimi Warkiani ◽  
Guofeng Guan ◽  
Khoo Bee Luan ◽  
Wong Cheng Lee ◽  
Ali Asgar S. Bhagat ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Label Free

scholarly journals Toward Microfluidic Label‐Free Isolation and Enumeration of Circulating Tumor Cells from Blood Samples

2019 ◽  
Vol 95 (10) ◽  
pp. 1085-1095 ◽  
Author(s):  
Camille Raillon ◽  
James Che ◽  
Sandy Thill ◽  
Margaux Duchamp ◽  
Benoît X. E. Desbiolles ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Label Free ◽  
Blood Samples
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