A new on-chip whole blood/plasma separator driven by asymmetric capillary forces

Lab on a Chip ◽  
2013 ◽  
Vol 13 (16) ◽  
pp. 3261 ◽  
Author(s):  
Kang Kug Lee ◽  
Chong H. Ahn
Keyword(s):  
Blood Plasma ◽  
Whole Blood ◽  
Capillary Forces ◽  
On Chip

On-chip whole blood plasma separator based on microfiltration, sedimentation and wetting contrast

2015 ◽  
Vol 22 (8) ◽  
pp. 2077-2085 ◽  
Author(s):  
Sanghoon Park ◽  
Roxana Shabani ◽  
Mark Schumacher ◽  
Yoon-Seoung Kim ◽  
Young Min Bae ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Whole Blood ◽  
On Chip

scholarly journals A Novel Microfluidic Device for Blood Plasma Filtration

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 336
Author(s):  
Zaidon T. Al-aqbi ◽  
Salim Albukhaty ◽  
Ameerah M. Zarzoor ◽  
Ghassan M. Sulaiman ◽  
Khalil A. A. Khalil ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Small Molecules ◽  
Microfluidic Device ◽  
Whole Blood ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Seminal Fluid ◽  
New Device ◽  
Plasma Filtration ◽  
On Chip

The use of whole blood and some biological specimens, such as urine, saliva, and seminal fluid are limited in clinical laboratory analysis due to the interference of proteins with other small molecules in the matrix and blood cells with optical detection methods. Previously, we developed a microfluidic device featuring an electrokinetic size and mobility trap (SMT) for on-chip extract, concentrate, and separate small molecules from a biological sample like whole blood. The device was used to on-chip filtrate the whole blood from the blood cells and plasma proteins and then on-chip extract and separate the aminoglycoside antibiotic drugs within 3 min. Herein, a novel microfluidic device featuring a nano-junction similar to those reported in the previous work formed by dielectric breakdown was developed for on-chip filtration and out-chip collection of blood plasma with a high extraction yield of 62% within less than 5 min. The filtered plasma was analyzed using our previous device to show the ability of this new device to remove blood cells and plasma proteins. The filtration device shows a high yield of plasma allowing it to detect a low concentration of analytes from the whole blood.

An on-chip whole blood/plasma separator with bead-packed microchannel on COC polymer

2010 ◽  
Vol 12 (5) ◽  
pp. 949-957 ◽  
Author(s):  
Joon S. Shim ◽  
Andrew W. Browne ◽  
Chong H. Ahn
Keyword(s):  
Blood Plasma ◽  
Whole Blood ◽  
On Chip

Comparison of whole blood, plasma and nails as monitors for the dietary intake of selenium

1998 ◽  
Vol 236 (1-2) ◽  
pp. 29-34 ◽  
Author(s):  
M. M. Mason ◽  
J. S. Morris ◽  
V. L. Spate ◽  
C. K. Baskett ◽  
T. A. Nichols ◽  
...  
Keyword(s):  
Dietary Intake ◽  
Blood Plasma ◽  
Whole Blood

scholarly journals Effect of the cholesterol content of small unilamellar liposomes on their stability in vivo and in vitro

1980 ◽  
Vol 186 (2) ◽  
pp. 591-598 ◽  
Author(s):  
Christopher Kirby ◽  
Jacqui Clarke ◽  
Gregory Gregoriadis
Keyword(s):  
Blood Plasma ◽  
Intravenous Injection ◽  
Whole Blood ◽  
Cholesterol Content ◽  
Molar Ratio ◽  
Phosphatidylcholine Liposomes ◽  
Effective Use ◽  
Positively Charged

Small unilamellar neutral, negatively and positively charged liposomes composed of egg phosphatidylcholine, various amounts of cholesterol and, when appropriate, phosphatidic acid or stearylamine and containing 6-carboxyfluorescein were injected into mice, incubated with mouse whole blood, plasma or serum or stored at 4°C. Liposomal stability, i.e. the extent to which 6-carboxyfluorescein is retained by liposomes, was dependent on their cholesterol content. (1) Cholesterol-rich (egg phosphatidylcholine/cholesterol, 7:7 molar ratio) liposomes, regardless of surface charge, remained stable in the blood of intravenously injected animals for up to at least 400min. In addition, stability of cholesterol-rich liposomes was largely maintained in vitro in the presence of whole blood, plasma or serum for at least 90min. (2) Cholesterol-poor (egg phosphatidylcholine/cholesterol, 7:2 molar ratio) or cholesterol-free (egg phosphatidylcholine) liposomes lost very rapidly (at most within 2min) much of their stability after intravenous injection or upon contact with whole blood, plasma or serum. Whole blood and to some extent plasma were less detrimental to stability than was serum. (3) After intraperitoneal injection, neutral cholesterol-rich liposomes survived in the peritoneal cavity to enter the blood circulation in their intact form. Liposomes injected intramuscularly also entered the circulation, although with somewhat diminished stability. (4) Stability of neutral and negatively charged cholesterol-rich liposomes stored at 4°C was maintained for several days, and by 53 days it had declined only moderately. Stored liposomes retained their unilamellar structure and their ability to remain stable in the blood after intravenous injection. (5) Control of liposomal stability by adjusting their cholesterol content may help in the design of liposomes for effective use in biological systems in vivo and in vitro.

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