Photodegradation kinetics of lodenafil carbonate, structure elucidation of two major degradation products using UPLC-MS/MS and in vitro cytotoxicity

2013 ◽  
Vol 5 (22) ◽  
pp. 6511 ◽  
Author(s):  
Cristiane Franco Codevilla ◽  
Alini Dall Cortivo Lange ◽  
Juliana Maria de Mello Andrade ◽  
Jéferson Segalin ◽  
Pedro Eduardo Fröehlich ◽  
...  
Keyword(s):  
Structure Elucidation ◽  
Degradation Products ◽  
In Vitro Cytotoxicity ◽  
Kinetics Of

In Vitro Cytotoxicity of Injectable and Biodegradable Poly(propylene fumarate)-Based Networks:  Unreacted Macromers, Cross-Linked Networks, and Degradation Products

2003 ◽  
Vol 4 (4) ◽  
pp. 1026-1033 ◽  
Author(s):  
Mark D. Timmer ◽  
Heungsoo Shin ◽  
R. Adam Horch ◽  
Catherine G. Ambrose ◽  
Antonios G. Mikos
Keyword(s):  
Degradation Products ◽  
In Vitro Cytotoxicity ◽  
Biodegradable Poly ◽  
Poly Propylene

Métabolisme de l'acide indolyl-3-acétique dans les cultures de cellules d'Acer pseudoplatanus cultivés in vitro

1977 ◽  
Vol 55 (19) ◽  
pp. 2530-2534 ◽  
Author(s):  
F. Maillard ◽  
J.-P. Zrÿd
Keyword(s):  
Cell Wall ◽  
Acetic Acid ◽  
Culture Medium ◽  
Degradation Products ◽  
Cell Suspensions ◽  
The Other ◽  
Acer Pseudoplatanus ◽  
Kinetics Of

Incubation of cell suspensions of sycamore (Acer pseudoplatanus) with β-indoyl-3-acetic acid (IAA) first led to the formation of IAA-glycosides, then to that of IAA-aspartate. Great differences are observed between the kinetics of IAA transformed by two distinct strains: one, auxin dependent (S), the other, auxin independent (MB). Other degradation products are only found in the culture medium. The localization of IAA-degrading systems in the cell wall is postulated. The auxin requirement of the S strain is discussed.

scholarly journals A fluorescent residualizing label for studies on protein uptake and catabolism in vivo and in vitro

1990 ◽  
Vol 267 (1) ◽  
pp. 155-162 ◽  
Author(s):  
J L Maxwell ◽  
L Terracio ◽  
T K Borg ◽  
J W Baynes ◽  
S R Thorpe
Keyword(s):  
Half Life ◽  
Normal Tissue ◽  
Degradation Products ◽  
Rat Serum ◽  
Peripheral Tissues ◽  
Protein Uptake ◽  
Kinetics Of ◽  
Rat Serum Albumin

Residualizing labels are tracers which remain in lysosomes after uptake and catabolism of the carrier protein and have been especially useful for studies on the sites of plasma protein degradation. Thus far these labels have contained radioactive reporters such as 3H or 125I. In the present paper we describe a fluorescent residualizing label, NN-dilactitol-N′-fluoresceinylethylenediamine (DLF). Modification of asialofetuin (ASF) or rat serum albumin (RSA) with DLF affected neither their normal kinetics of clearance from the rat circulation nor their normal tissue sites of uptake and degradation. After injection of DLF-ASF, fluorescent degradation products were recovered nearly quantitatively in liver and retained with a half-life of about 2 days. Fluorescent degradation products from DLF-RSA were recovered in skin and muscle, and were localized in fibroblasts by fluorescence microscopy. These results confirm previous studies with radioactive residualizing labels in which fibroblasts in peripheral tissues were identified as primary sites of albumin degradation. Fluorescent catabolites also accumulated in fibroblasts incubated with DLF-RSA in vitro, and residualized with a half-life of about 2 days. Overall, the data establish that DLF functions efficiently as a fluorescent residualizing label both in vivo and in vitro. The advantages of fluorescent, compared with radioactive, residualizing labels should make them valuable tools for studies on protein uptake and catabolism in biological systems.

scholarly journals Identification, isolation, structural characterization, in silico toxicity prediction and in vitro cytotoxicity assay of simeprevir acidic and oxidative degradation products

RSC Advances ◽  
2020 ◽  
Vol 10 (70) ◽  
pp. 42816-42826
Author(s):  
Rasha M. Ahmed ◽  
Marwa A. A. Fayed ◽  
Mohammed F. El-Behairy ◽  
Inas A. Abdallah
Keyword(s):  
Chronic Hepatitis ◽  
Degradation Products ◽  
Antiviral Drug ◽  
Oxidative Degradation ◽  
In Vitro Cytotoxicity ◽  
Toxicity Prediction ◽  
Direct Acting Antiviral ◽  
In Silico Toxicity ◽  
Direct Acting

Simeprevir is a new direct-acting antiviral drug used for the treatment of chronic hepatitis C.

Isolation, characterization using LC-ESI-QTOF, NMR and in vitro cytotoxicity assay of niclosamide forced degradation products

2017 ◽  
Vol 136 ◽  
pp. 148-155 ◽  
Author(s):  
Johnsirani P. ◽  
Vishnuvardhan Ch. ◽  
Lingesh A. ◽  
Naidu V.G.M. ◽  
Naveen Ch. ◽  
...  
Keyword(s):  
Degradation Products ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Assay ◽  
Forced Degradation

Photodegradation kinetics of lisdexamfetamine dimesylate and structure elucidation of its degradation products by LC-ESI-QTOF

2018 ◽  
Vol 10 (19) ◽  
pp. 2287-2292 ◽  
Author(s):  
Graciela Carlos ◽  
Jéferson Segalin ◽  
Louise Jank ◽  
Eloisa Comiran ◽  
Marcella Herbstrith de Oliveira ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Structure Elucidation ◽  
Degradation Products ◽  
Lisdexamfetamine Dimesylate ◽  
Kinetics Of

Herein, we investigate the photodegradation of lisdexamfetamine (LDX) in an aqueous solution.

Delapril and Manidipine Main Degradation Products: LC-UV and LC-ESI-MS Evaluations, Decay Kinetic, and in vitro Cytotoxicity Studies

2015 ◽  
Vol 38 (13) ◽  
pp. 1333-1342 ◽  
Author(s):  
Vítor Todeschini ◽  
Maximiliano da Silva Sangoi ◽  
Gustavo Krumel Goelzer ◽  
Juliana Maria de Mello Andrade ◽  
Nadia Maria Volpato
Keyword(s):  
Degradation Products ◽  
In Vitro Cytotoxicity ◽  
Decay Kinetic ◽  
Esi Ms ◽  
Cytotoxicity Studies
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