Biotin labeling and receptor analysis of huwentoxin-I

2013 ◽  
Vol 5 (16) ◽  
pp. 3888 ◽  
Author(s):  
Yi-Zhong Yan ◽  
Hui Liu ◽  
Rong Lv ◽  
Sha Huang ◽  
Ji-Rong Wang ◽  
...  
Keyword(s):  
Biotin Labeling

scholarly journals Nuclear Run-On Assay Using Biotin Labeling, Magnetic Bead Capture and Analysis by Fluorescence-Based RT-PCR

BioTechniques ◽  
2000 ◽  
Vol 29 (5) ◽  
pp. 1012-1017 ◽  
Author(s):  
G. Patrone ◽  
F. Puppo ◽  
R. Cusano ◽  
M. Scaranari ◽  
I. Ceccherini ◽  
...  
Keyword(s):  
Magnetic Bead ◽  
Rt Pcr ◽  
Biotin Labeling

Availability of NHS-biotin labeling to identify free protein lysine revealed by experiment and MD simulation

2018 ◽  
Vol 557 ◽  
pp. 46-58
Author(s):  
Aiichiro Muraoka ◽  
Yoshinori Matsuura ◽  
Hisashi Naitow ◽  
Makoto Ihara ◽  
Naoki Kunishima
Keyword(s):  
Md Simulation ◽  
Free Protein ◽  
Biotin Labeling

Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing

2011 ◽  
Vol 74 (10) ◽  
pp. 1985-1993 ◽  
Author(s):  
Kohji Nagano ◽  
Takashi Shinkawa ◽  
Kuniyasu Kato ◽  
Noriyuki Inomata ◽  
Nami Yabuki ◽  
...  
Keyword(s):  
Cell Surface ◽  
Proteome Profiling ◽  
Distinct Cell ◽  
Biotin Labeling ◽  
Surface Proteome ◽  
Cell Surface Proteome

Synthesis of biotin–AMP conjugate for 5′ biotin labeling of RNA through one-step in vitro transcription

2008 ◽  
Vol 3 (12) ◽  
pp. 1848-1861 ◽  
Author(s):  
Faqing Huang ◽  
Jun He ◽  
Yilin Zhang ◽  
Yanlin Guo
Keyword(s):  
In Vitro Transcription ◽  
Biotin Labeling ◽  
One Step

scholarly journals An improved smaller biotin ligase for BioID proximity labeling

2016 ◽  
Vol 27 (8) ◽  
pp. 1188-1196 ◽  
Author(s):  
Dae In Kim ◽  
Samuel C. Jensen ◽  
Kyle A. Noble ◽  
Birendra KC ◽  
Kenneth H. Roux ◽  
...  
Keyword(s):  
Fusion Proteins ◽  
Living Cells ◽  
Biotin Ligase ◽  
Selective Targeting ◽  
Biotin Labeling ◽  
Biotin Supplementation ◽  
Application Specific

The BioID method uses a promiscuous biotin ligase to detect protein–protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate proteins. Thus BioID2 improves the efficiency of screening for protein–protein associations. We also demonstrate that the biotinylation range of BioID2 can be considerably modulated using flexible linkers, thus enabling application-specific adjustment of the biotin-labeling radius.

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