Synthesis of biotin–AMP conjugate for 5′ biotin labeling of RNA through one-step in vitro transcription

2008 ◽  
Vol 3 (12) ◽  
pp. 1848-1861 ◽  
Author(s):  
Faqing Huang ◽  
Jun He ◽  
Yilin Zhang ◽  
Yanlin Guo
Keyword(s):  
In Vitro Transcription ◽  
Biotin Labeling ◽  
One Step

THE HISTOLOGICAL LOCALIZATION OF FasL mRNA IN HUMAN LUMBAR DISC TISSUE

2006 ◽  
Vol 10 (02) ◽  
pp. 89-94
Author(s):  
Fagang Ye ◽  
Chunxiang Si ◽  
Yujin Qiu ◽  
Bohua Chen ◽  
Zhenhua Lu ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Lumbar Disc ◽  
In Vitro Transcription ◽  
Lumbar Intervertebral Disc ◽  
Degenerative Disc ◽  
Disc Cells ◽  
One Step ◽  
Notochord Cells ◽  
Fasl Mrna

Lumbar disc has become a degenerative tissue since teenage. Apoptosis plays an important role in the processes of degeneration. FasL, a ligand of Fas receptor (a trans-membrane protein) promote signal transduction of apoptosis. In this study, fetal disc and degenerative disc from adults were examined for FasL mRNA expression. hPBMC was first treated by PHA-P, a fragment of FasL cDNA was then synthesized through one-step RT-PCR. Verified by sequencing and recombinant plasmids linearizing, the DIG-cRNA probe was synthesized by in vitro transcription. The distribution of FasL mRNA was observed on lumbar intervertebral disc specimens using in situ hybridization. FasL mRNA signals were expressed in both notochord cells and chondrocyte-like cells in the nucleus of fetal discs. In degenerative disc, the quantity of intervertebral disc cells reduced dramatically and the expression of FasL mRNA was not detected. Expression of FasL in fetal disc implied that Fas/FasL triggered cell death signal pathway involved in lumbar disk transition and degeneration.

In vitro transcription and translation of simian rotavirus SA11 gene products.

1980 ◽  
Vol 33 (3) ◽  
pp. 1111-1121 ◽  
Author(s):  
B B Mason ◽  
D Y Graham ◽  
M K Estes
Keyword(s):  
In Vitro Transcription ◽  
Gene Products
Sign in / Sign up

Export Citation Format

Share Document