Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platform

The Analyst ◽  
2013 ◽  
Vol 138 (6) ◽  
pp. 1844 ◽  
Author(s):  
Fang Li ◽  
Yuqi Yu ◽  
Hua Cui ◽  
Di Yang ◽  
Zhiping Bian
Keyword(s):  
Gold Nanoparticles ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Cardiac Troponin I ◽  
Label Free ◽  
Functionalized Gold Nanoparticles ◽  
Sensing Platform

Gold nanoparticles mediated label-free capacitance detection of cardiac troponin I

2012 ◽  
Vol 161 (1) ◽  
pp. 761-768 ◽  
Author(s):  
Vijayender Bhalla ◽  
Sandro Carrara ◽  
Priyanka Sharma ◽  
Yogesh Nangia ◽  
C. Raman Suri
Keyword(s):  
Gold Nanoparticles ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Cardiac Troponin I ◽  
Label Free ◽  
Capacitance Detection

Simple, low-cost, sensitive and label-free aptasensor for the detection of cardiac troponin I based on a gold nanoparticles modified titanium foil

2019 ◽  
Vol 126 ◽  
pp. 381-388 ◽  
Author(s):  
Nasrin Siraj Lopa ◽  
Md. Mahbubur Rahman ◽  
Faiz Ahmed ◽  
Taewook Ryu ◽  
Sabuj Chandra Sutradhar ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Low Cost ◽  
Titanium Foil ◽  
Cardiac Troponin I ◽  
Label Free

scholarly journals Fluorescence Based on Surface Plasmon Coupled Emission for Ultrahigh Sensitivity Immunoassay of Cardiac Troponin I

Biomedicines ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 448
Author(s):  
Vien Thi Tran ◽  
Heongkyu Ju
Keyword(s):  
Surface Plasmon ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Limit Of Detection ◽  
Delayed Diagnosis ◽  
Cardiac Troponin I ◽  
Quantitative Detection ◽  
Fluorescence Signal ◽  
Label Free ◽  
Ultrahigh Sensitivity

This work demonstrates the quantitative assay of cardiac Troponin I (cTnI), one of the key biomarkers for acute cardiovascular diseases (the leading cause of death worldwide) using the fluorescence-based sandwich immune reaction. Surface plasmon coupled emission (SPCE) produced by non-radiative coupling of dye molecules with surface plasmons being excitable via the reverse Kretschmann format is exploited for fluorescence-based sandwich immunoassay for quantitative detection of cTnI. The SPCE fluorescence chip utilizes the gold (2 nm)-silver (50 nm) bimetallic thin film, with which molecules of the dye Alexa 488 (conjugated with detection antibodies) make a near field coupling with the plasmonic film for SPCE. The experimental results find that the SPCE greatly improves the sensitivity via enhancing the fluorescence signal (up to 50-fold) while suppressing the photo-bleaching, permitting markedly enhanced signal-to-noise ratio. The limit of detection of 21.2 ag mL−1 (atto-gram mL−1) is obtained, the lowest ever reported to date amid those achieved by optical technologies such as luminescence and label-free optical sensing techniques. The features discovered such as ultrahigh sensitivity may prompt the presented technologies to be applied for early diagnosis of cTnI in blood, particularly for emergency medical centers overloaded with patients with acute myocardial infarction who would suffer from time-delayed diagnosis due to insufficient assay device sensitivity.

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