Site-directed antibody immobilization using a protein A–gold binding domain fusion protein for enhanced SPR immunosensing

The Analyst ◽  
2013 ◽  
Vol 138 (7) ◽  
pp. 2023 ◽  
Author(s):  
Elena de Juan-Franco ◽  
Antonio Caruz ◽  
J. R. Pedrajas ◽  
Laura M. Lechuga
Keyword(s):  
Fusion Protein ◽  
Protein A ◽  
Binding Domain ◽  
Antibody Immobilization ◽  
Domain Fusion

scholarly journals Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor‒cellulose-binding domain fusion protein

1999 ◽  
Vol 339 (2) ◽  
pp. 429 ◽  
Author(s):  
J. Greg DOHENY ◽  
Eric J. JERVIS ◽  
M. Marta GUARNA ◽  
R. Keith HUMPHRIES ◽  
R. Antony J. WARREN ◽  
...  
Keyword(s):  
Stem Cell ◽  
Fusion Protein ◽  
Stem Cell Factor ◽  
Binding Domain ◽  
Target Cells ◽  
Cellulose Binding Domain ◽  
Inert Matrix ◽  
Domain Fusion ◽  
Cellulose Binding

Obtaining immunoglobulin-binding sorbent by immobilization of recombinant human proinsulin protein A domain fusion protein

1994 ◽  
Vol 8 (11) ◽  
pp. 805-810
Author(s):  
S. N. Eremenko ◽  
D. M. Koulich ◽  
V. E. Petukhov ◽  
K. V. Maltsev ◽  
A. N. Wulfson ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Protein A ◽  
Human Proinsulin ◽  
Domain Fusion ◽  
Immunoglobulin Binding

A streptavidin-cellulose-binding domain fusion protein that binds biotinylated proteins to cellulose

1994 ◽  
Vol 16 (6) ◽  
pp. 496-500 ◽  
Author(s):  
Khai D. Le ◽  
Neil R. Gilkes ◽  
Douglas G. Kilburn ◽  
Robert C. Miller ◽  
John N. Saddler ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Domain Fusion ◽  
Cellulose Binding

Crystallization of an intact GST-estrogen receptor hormone binding domain fusion protein

1998 ◽  
Vol 54 (3) ◽  
pp. 423-426 ◽  
Author(s):  
John M. Lally ◽  
Richard H. Newman ◽  
Philip P. Knowles ◽  
Suhail Islam ◽  
Arnold I. Coffer ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Fusion Protein ◽  
Binding Domain ◽  
Thin Plates ◽  
Hormone Binding ◽  
X Ray Diffraction ◽  
Visible Image ◽  
Domain Fusion ◽  
Hormone Binding Domain ◽  
Gst Fusion Proteins

Crystals of an intact GST–estrogen receptor hormone binding domain fusion protein have been grown from solutions of MPD. The crystals grew as clusters of thin plates and needles of maximum dimensions 100 × 20 × 1 µm but were unsuitable for X-ray diffraction analysis. However, examination by electron microscopy shows an ordered lattice in which the protein molecules are clearly visible. Image analysis of electron micrographs of the protein crystals revealed electron stain-excluding density which showed a two-domain trimeric structure in projection, with each molecule of dimensions 12.0 × 5.0 nm diameter. The use of GST-fusion proteins in crystallization are discussed.

scholarly journals Efficient siRNA delivery into primary cells by a peptide transduction domain–dsRNA binding domain fusion protein

2009 ◽  
Vol 27 (6) ◽  
pp. 567-571 ◽  
Author(s):  
Akiko Eguchi ◽  
Bryan R Meade ◽  
Yung-Chi Chang ◽  
Craig T Fredrickson ◽  
Karl Willert ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Sirna Delivery ◽  
Binding Domain ◽  
Primary Cells ◽  
Domain Fusion ◽  
Dsrna Binding ◽  
Peptide Transduction
Sign in / Sign up

Export Citation Format

Share Document