scholarly journals Efficient siRNA delivery into primary cells by a peptide transduction domain–dsRNA binding domain fusion protein

2009 ◽  
Vol 27 (6) ◽  
pp. 567-571 ◽  
Author(s):  
Akiko Eguchi ◽  
Bryan R Meade ◽  
Yung-Chi Chang ◽  
Craig T Fredrickson ◽  
Karl Willert ◽  
...  
Author(s):  
Zarel Martinez ◽  
Kristof De Schutter ◽  
Els J.M. Van Damme ◽  
Elise Vogel ◽  
Niels Wynant ◽  
...  

1999 ◽  
Vol 339 (2) ◽  
pp. 429 ◽  
Author(s):  
J. Greg DOHENY ◽  
Eric J. JERVIS ◽  
M. Marta GUARNA ◽  
R. Keith HUMPHRIES ◽  
R. Antony J. WARREN ◽  
...  

1994 ◽  
Vol 16 (6) ◽  
pp. 496-500 ◽  
Author(s):  
Khai D. Le ◽  
Neil R. Gilkes ◽  
Douglas G. Kilburn ◽  
Robert C. Miller ◽  
John N. Saddler ◽  
...  

1998 ◽  
Vol 54 (3) ◽  
pp. 423-426 ◽  
Author(s):  
John M. Lally ◽  
Richard H. Newman ◽  
Philip P. Knowles ◽  
Suhail Islam ◽  
Arnold I. Coffer ◽  
...  

Crystals of an intact GST–estrogen receptor hormone binding domain fusion protein have been grown from solutions of MPD. The crystals grew as clusters of thin plates and needles of maximum dimensions 100 × 20 × 1 µm but were unsuitable for X-ray diffraction analysis. However, examination by electron microscopy shows an ordered lattice in which the protein molecules are clearly visible. Image analysis of electron micrographs of the protein crystals revealed electron stain-excluding density which showed a two-domain trimeric structure in projection, with each molecule of dimensions 12.0 × 5.0 nm diameter. The use of GST-fusion proteins in crystallization are discussed.


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